Bacteriolyses of Bacterial Cell Walls by Cu(II) and Zn(II) Ions Based on Antibacterial Results of Dilution Medium Method and Halo Antibacterial Test

Ishida, Sci. Tsuneo

doi:10.15226/2475-4714/2/2/00120

Cited by 17 publications

(7 citation statements)

References 36 publications

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“…The results of the antibacterial activity of Gram-negative Klebsiella pneumoniae and Gram-positive Staphylococcus aureus after immersing LbL-treated fabric into Cu 2+ solution are summarized in Table 6. Metal ions such as Cu 2+ and Zn 2+ damage the cell membrane acting as a biosynthesis inhibitor, thus killing the bacteria [35]. As expected, all PA/CHurea + Cu 2+ treated samples killed almost 100% of the bacteria.…”

Section: Resultssupporting

confidence: 70%

Environmentally Benign Phytic Acid-Based Nanocoating for Multifunctional Flame-Retardant/Antibacterial Cotton

Magovac

Vončina

Budimir

et al. 2021

Fibers

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Environmentally benign layer-by-layer (LbL) deposition was used to obtain flame-retardant and antimicrobial cotton. Cotton was coated with 8, 10, and 12 phytic acid (PA) and chitosan (CH)-urea bilayers (BL) and then immersed into copper (II) sulfate (CuSO4) solution. Our findings were that 12 BL of PA/CH-urea + Cu2+ were able to stop flame on cotton during vertical flammability testing (VFT) with a limiting oxygen index (LOI) value of 26%. Microscale combustion calorimeter (MCC) data showed a reduction of peak heat release rates (pHRR) of more than 61%, while the reduction of total heat release (THR) was more than 54%, relative to untreated cotton. TG-IR analysis of 12 BL-treated cotton showed the release of water, methane, carbon dioxide, carbon monoxide, and aldehydes, while by adding Cu2+ ions, the treated cotton produces a lower amount of methane. Treated cotton also showed no levoglucosan. The intumescent behavior of the treatment was indicated by the bubbled structure of the post-burn char. Antibacterial testing showed a 100% reduction of Klebsiella pneumoniae and Staphylococcus aureus. In this study, cotton was successfully functionalized with a multifunctional ecologically benign flame-retardant and antibacterial nanocoating, by means of LbL deposition.

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Section: Resultssupporting

confidence: 70%

Environmentally Benign Phytic Acid-Based Nanocoating for Multifunctional Flame-Retardant/Antibacterial Cotton

Magovac

Vončina

Budimir

et al. 2021

Fibers

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“…45 In case of E. coli , bacteriolysis occurs due to destruction of cell wall structure by degrading of lipoprotein at C-, N-terminals, owing to peptidoglycan formation inhibition. 45…”

Section: Resultsmentioning

confidence: 99%

“…32 Zinc ions released from the mats react with the surface of S. aureus forming zinc proteins due to formation Zn-cysteine complex in bacterium which results in the cell wall destruction by the inhibition of peptidoglycan elongation. 45 In case of E. coli, bacteriolysis occurs due to destruction of cell wall structure by degrading of lipoprotein at C-, N-terminals, owing to peptidoglycan formation inhibition. 45 Cellular viability and proliferation.…”

Section: Antibacterial Propertiesmentioning

confidence: 99%

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Enhanced mesenchymal stem cells growth on antibacterial microgrooved electrospun zinc chloride/polycaprolactone conduits for peripheral nerve regeneration

Ekram

El‐Hady

El-Kady

et al. 2021

Journal of Bioactive and Compatible Polymers

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In this study, we have investigated the effect of adding zinc chloride (ZnCl2) on polycaprolactone (PCL) before and after electrospinning. The rheological properties and conductivity of ZnCl2/PCL solutions were measured prior to the electrospinning process. The resultant electrospun mats were characterized by SEM, contact angle, FTIR, XRD, mechanical properties, as well as its antibacterial and stem cell proliferation assessment were tested. It was found that the fibers became finer by increasing the zinc salt content. Moreover, stability increased slightly up to 5% Zn-PCL and also the hydrophilicity has been enhanced by 52%. By adding ZnCl2, the degradation rate and mechanical properties were significantly increased. Also, the resultant mats have shown antibacterial properties against S. aureus than E. coli. From the stem cells proliferation study, it can be observed that by increasing ZnCl2, the stem cells proliferation was significantly increased. Grooved multichannel nerve conduits were successfully fabricated by rolling the electrospun mats produced on corn husks which has shown better cell alignment and attachment. Hence, adding zinc chloride is a facile biocompatible enhancement to polycaprolactone nanofibers to be used in nerve regeneration.

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“…The ion can also disrupt the iron-sulfur (cysteine) clusters that are present in many physiologically essential biosynthetic and catabolic enzymes, releasing the Fenton-active Fe(II) ion, with the latter further reacting with cellular H 2 O 2 to form hydroxyl radical [45]. Cu(II) ion has also been indicated to target cell envelopes, disrupting functional groups in peptidoglycan (peptidoglycan is also present in Gram-negative bacteria, as thin layer in between the outer and inner membranes) and phospholipids, the latter affecting membrane permeability [46,47]. The implied leached soluble Cu-mediated cellular hydroxyl radical generation and cell envelope targeting are consistent with the damaged membrane observation, herein also evident with the CuO150-exposed bacterial samples (Figure 4(b)).…”

Section: Antibacterial Efficacies Of Cu X P Particles and Mechanisticmentioning

confidence: 99%

Hydroxyl Radical Generating Monovalent Copper Particles for Antimicrobial Application

Tan

Zhang

Xie

et al. 2023

Journal of Nanomaterials

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The antimicrobial properties of copper are well-known but maintaining a low oxidation state of Cu in particles is difficult. Herein, antimicrobial CuxP particles were synthesized through phosphorization of Cu(OH)2, to lock copper in its monovalent state (as Cu3P). We found that the phosphorization could be achieved at temperatures as low as 200°C, with stable surface presence of Cu(I) on the resulting CuxP particles. Cu(I) can act as a one-electron reducing agent for molecular oxygen, to generate the highly reactive hydroxyl radical. In this study, CuxP displayed antibacterial activities on the Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, with minimum inhibitory concentrations of 32 mg/L for the highest temperature particles (350°C) on both model bacteria. The evident membrane damage is consistent with the intended hydroxyl radical bacterial targeting mechanism. Low-temperature CuxP, although exhibiting lower antibacterial efficacies than those of the higher temperature variant, still showed competitive growth inhibiting activities when compared to other reported antimicrobial copper-based particles. The present work showcases advancements in particle technology that can lead to the development of a more robust antimicrobial agent, presenting a potent additive for self-disinfection applications.

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Bacteriolyses of Bacterial Cell Walls by Cu(II) and Zn(II) Ions Based on Antibacterial Results of Dilution Medium Method and Halo Antibacterial Test

Abstract: Bacteriolyses of bacterial cell walls by copper (II)

Cited by 17 publications

References 36 publications

Environmentally Benign Phytic Acid-Based Nanocoating for Multifunctional Flame-Retardant/Antibacterial Cotton

Environmentally Benign Phytic Acid-Based Nanocoating for Multifunctional Flame-Retardant/Antibacterial Cotton

Enhanced mesenchymal stem cells growth on antibacterial microgrooved electrospun zinc chloride/polycaprolactone conduits for peripheral nerve regeneration

Hydroxyl Radical Generating Monovalent Copper Particles for Antimicrobial Application

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