Bacteriophage cocktail for biocontrol of soft rot disease caused by Pectobacterium species in Chinese cabbage

Vu, Nguyen Trung; Kim, Hyeongsoon; Lee, Soohong; Hwang, In Sun; Kwon, Choon-Tak; Oh, Chang-Sik

doi:10.1007/s00253-023-12881-x

Cited by 6 publications

(3 citation statements)

References 54 publications

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“…Traditional antimicrobial agents, such as copper-based bactericides and antibiotics, have been used for controlling soft rot-bacteria. However, their use has been limited due to microbial resistance, accumulation on plants, and potential hazards to human health ( Abd-EI-Khair et al, 2021 ; Vu et al, 2024 ). In recent years, there has been growing interest in biological control methods to manage bacterial soft rot.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, there has been growing interest in biological control methods to manage bacterial soft rot. These approaches involve the application of beneficial microorganisms such as Trichoderma spp., Bacillus spp., Pseudomonas spp., Lactobacillus spp., and bacteriophages ( Tsuda et al, 2016 ; Abd-EI-Khair et al, 2021 ; Vu et al, 2024 ). These biological control agents can help combat soft rot by competing with the pathogens, producing antimicrobial compounds, or inducing plant defenses.…”

Section: Discussionmentioning

confidence: 99%

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Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone

Li,

Ren,

Ibrahim

et al. 2024

Front. Microbiol.

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Chinese cabbage, scientifically known as Brassica rapa subsp. pekinensis, is a highly popular vegetable in China for its delectable taste. However, the occurrence of bacterial soft rot disease poses a significant threat to its growth and overall development. Consequently, this study aimed to explore the defense mechanisms employed by Chinese cabbage against bacterial soft rot disease. Specifically, the investigation focused on understanding the relationship between the disease and the microbial communities present in the soil surrounding the roots of Chinese cabbage. Significant disparities were observed in the composition of microbial communities present in the root-zone soil of healthy Chinese cabbage plants compared to those affected by Pectobacterium brasiliense-caused soft rot disease. The analysis of 16S rRNA gene high-throughput sequencing results revealed a lower abundance of Proteobacteria (8.39%), Acidobacteriot (0.85), Sphingomonas (3.51%), and Vicinamibacteraceae (1.48%), whereas Firmicutes (113.76%), Bacteroidota (8.71%), Chloroflexi (4.89%), Actinobacteriota (1.71%), A4b (15.52%), Vicinamibacterales (1.62%), and Gemmatimonadaceae (1.35%) were more prevalent in healthy plant soils. Similarly, the analysis of ITS gene high-throughput sequencing results indicated a reduced occurrence of Chytridiomycota (23.58%), Basidiomycota (21.80%), Plectosphaerella (86.22%), and Agaricomycetes (22.57%) in healthy soils. In comparison, Mortierellomycota (50.72%), Ascomycota (31.22%), Podospora (485.08%), and Mortierella (51.59%) were more abundant in healthy plant soils. In addition, a total of 15 bacterial strains were isolated from the root-zone soil of diseased Chinese cabbage plants. These isolated strains demonstrated the ability to fix nitrogen (with the exception of ZT20, ZT26, ZT41, ZT45, and ZT61), produce siderophores and indole acetic acid (IAA), and solubilize phosphate. Notably, ZT14 (Citrobacter freundii), ZT33 (Enterobacter cloacae), ZT41 (Myroides odoratimimus), ZT52 (Bacillus paramycoides), ZT58 (Klebsiella pasteurii), ZT45 (Klebsiella aerogenes), and ZT32 (Pseudomonas putida) exhibited significant growth-promoting effects as determined by the plant growth promotion (PGP) tests. Consequently, this investigation not only confirmed the presence of the soft rot pathogen in Chinese cabbage plants in Hangzhou, China, but also advanced our understanding of the defense mechanisms employed by Chinese cabbage to combat soft rot-induced stress. Additionally, it identified promising plant-growth-promoting microbes (PGPMs) that could be utilized in the future to enhance the Chinese cabbage industry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone

Li,

Ren,

Ibrahim

et al. 2024

Front. Microbiol.

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“…actinidiae : [9]; Pectobacterium spp. [10]). The use of phages against bacterial diseases in agriculture was proposed very early on after their discovery [11–14].…”

Section: Introductionmentioning

confidence: 99%

Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes

Feltin,

Garneau,

Morris

et al. 2024

Journal of General Virology

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Relatively few phages that infect plant pathogens have been isolated and investigated. The Pseudomonas syringae species complex is present in various environments, including plants. It can cause major crop diseases, such as bacterial canker on apricot trees. This study presents a collection of 25 unique phage genomes that infect P. syringae. These phages were isolated from apricot orchards with bacterial canker symptoms after enrichment with 21 strains of P. syringae. This collection comprises mostly virulent phages, with only three being temperate. They belong to 14 genera, 11 of which are newly discovered, and 18 new species, revealing great genetic diversity within this collection. Novel DNA packaging systems have been identified bioinformatically in one of the new phage species, but experimental confirmation is required to define the precise mechanism. Additionally, many phage genomes contain numerous potential auxiliary metabolic genes with diversified putative functions. At least three phages encode genes involved in bacterial tellurite resistance, a toxic metalloid. This suggests that viruses could play a role in bacterial stress tolerance. This research emphasizes the significance of continuing the search for new phages in the agricultural ecosystem to unravel novel ecological diversity and new gene functions. This work contributes to the foundation for future fundamental and applied research on phages infecting phytopathogenic bacteria.

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Microbial community structure of an anaerobic side-stream coupled anoxic–aerobic membrane bioreactor (AOMBR–ASSR) for an in-situ sludge reduction process

Ning,

Hu,

Yue

et al. 2024

Bioprocess Biosyst Eng

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Bacteriophage cocktail for biocontrol of soft rot disease caused by Pectobacterium species in Chinese cabbage

Cited by 6 publications

References 54 publications

Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone

Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone

Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes

Microbial community structure of an anaerobic side-stream coupled anoxic–aerobic membrane bioreactor (AOMBR–ASSR) for an in-situ sludge reduction process

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