Bacteriophage endolysins: A novel anti-infective to control Gram-positive pathogens

Fischetti, Vincent A.

doi:10.1016/j.ijmm.2010.04.002

Cited by 369 publications

(323 citation statements)

References 56 publications

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“…The absence of bacterial resistance against lysin is considered as a major advantage of using phage lysins (Fischetti 2010), as the bacterial cell would have to modify the structure of its cell wall to avoid enzymatic action. It was found that exposing bacteria to a particular lysin for 40 reproductive cycles did not give any resistant strains (Fischetti 2010).…”

Section: Application Of Phage Lysin In Foodmentioning

confidence: 99%

“…It was found that exposing bacteria to a particular lysin for 40 reproductive cycles did not give any resistant strains (Fischetti 2010). However, the production of lysin is expensive and, moreover, they are relatively unstable large proteins that are prone to proteolysis and lose its activity in some foods (Coffey et al 2010).…”

Section: Application Of Phage Lysin In Foodmentioning

confidence: 99%

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Bacteriophage biocontrol of foodborne pathogens

2015

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Bacteriophages are viruses that only infect bacterial cells. Phages are categorized based on the type of their life cycle, the lytic cycle cause lysis of the bacterium with the release of multiple phage particles where as in lysogenic phase the phage DNA is incorporated into the bacterial genome. Lysogeny does not result in lysis of the host. Lytic phages have several potential applications in the food industry as biocontrol agents, biopreservatives and as tools for detecting pathogens. They have also been proposed as alternatives to antibiotics in animal health. Two unique features of phage relevant for food safety are that they are harmless to mammalian cells and high host specificity, keeping the natural microbiota undisturbed. However, the recent approval of bacteriophages as food additives has opened the discussion about 'edible viruses'. This article reviews in detail the application of phages for the control of foodborne pathogens in a process known as Bbiocontrol^.

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Section: Application Of Phage Lysin In Foodmentioning

confidence: 99%

Section: Application Of Phage Lysin In Foodmentioning

confidence: 99%

Bacteriophage biocontrol of foodborne pathogens

2015

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“…Bacteriophages infecting Gram-positive bacteria harbour endolysins that are modular in nature, and contain at least one catalytic and one CBD (Fischetti, 2005(Fischetti, , 2010Sudiarta et al, 2010). However, endolysins of phages infecting Gram-negative bacteria contain only a catalytic domain (Cheng et al, 1994).…”

Section: Regulation By Specificitymentioning

confidence: 99%

“…Bacteriophages are able to infect and lyse bacterial cells, and it is this feature that makes them important antimicrobial agents (Sulakvelidze et al, 2001;Hermoso et al, 2007), especially because of the rapid emergence of multidrug-resistant bacteria (Loeffler et al, 2001;Schuch et al, 2002;O'Flaherty et al, 2009;Fischetti, 2010). Bacteriophages use two independent mechanisms to lyse host cells.…”

Section: Introductionmentioning

confidence: 99%

Insights into the regulation of bacteriophage endolysin: multiple means to the same end

Pohane¹,

Jain²

2015

Microbiology

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Antibiotics are the molecules of choice to treat bacterial infections. However, because of the rapid emergence of drug-resistant bacteria, alternative modes of combating infections are being envisaged. Bacteriophages, which infect and lyse bacterial cells, may function as effective antimicrobial agents. Most bacteriophages produce their own peptidoglycan hydrolase called endolysin or lysin, which breaks down the cell wall of bacteria and aids in the release of newly assembled virions. Here, we discuss several findings that help us in understanding how endolysins are regulated. We observe that there is no common mechanism that is followed in all cases. Many different modes of activity regulation have been observed in endolysins, including regulation of protein expression, translocation across the cell membrane and post-translational modifications. These processes not only demonstrate how endolysins are made dependent on other accessory proteins and non-protein factors for their synthesis, translocation across the cytoplasmic membrane and activity, but also show how autoregulation helps in maintaining the enzyme in an inactive form. Various regulatory mechanisms that are discussed are particularly applicable to endolysins. Nevertheless, a detailed study of these methods opens new avenues of investigation in the area of protein translocation systems and the novel ways of enzyme activation and regulation in bacteria.

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“…23 Unique endolysins that kill bacteria could play a major role in human and animal health and in food safety. 24 This list goes on.…”

Section: Student Discoveries Must Be Significant and Continue To Be Umentioning

confidence: 99%

Phage on the stage

Temple¹,

Lewis

2015

Bacteriophage

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Bacteriophage endolysins: A novel anti-infective to control Gram-positive pathogens

Cited by 369 publications

References 56 publications

Bacteriophage biocontrol of foodborne pathogens

Bacteriophage biocontrol of foodborne pathogens

Insights into the regulation of bacteriophage endolysin: multiple means to the same end

Phage on the stage

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