Bacteriophage lambda hin function

Court, Donald L.; Gottesman, Max E.; Gallo, Maria

doi:10.1016/0022-2836(80)90061-3

Cited by 11 publications

(1 citation statement)

References 23 publications

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“…However, this filamentation is quite different from that of the converted lysogen as pantoyl lactone could induce division in the former but not in the latter (Section 2.2.2). The hin gene product was shown to inhibit the transport of amino acids and nucleotides into the cell [48], believed to be caused by an alteration of the cytoplasmic membrane. The possibility that kil or hin gene products are expressed in appreciable amounts in the converted polylysogen has not been tested.…”

Section: Summary and Discussionmentioning

confidence: 99%

Altered Growth and Envelope Properties of Polylysogens Containing Bacteriophage Lambda N−cI− Prophages

Barik

Mandal

2020

IJMS

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The bacterial virus lambda (λ) is a temperate bacteriophage that can lysogenize host Escherichia coli (E. coli) cells. Lysogeny requires λ repressor, the cI gene product, which shuts off transcription of the phage genome. The λ N protein, in contrast, is a transcriptional antiterminator, required for expression of the terminator-distal genes, and thus, λ N mutants are growth-defective. When E. coli is infected with a λ double mutant that is defective in both N and cI (i.e., λN -cI -), at high multiplicities of 50 or more, it forms polylysogens that contain 20-30 copies of the λN -cIgenome integrated in the E. coli chromosome. Early studies revealed that the polylysogens underwent "conversion" to long filamentous cells that form tiny colonies on agar. Here, we report a large set of altered biochemical properties associated with this conversion, documenting an overall degeneration of the bacterial envelope. These properties reverted back to those of nonlysogenic E. coli as the metastable polylysogen spontaneously lost the λN -cIgenomes, suggesting that conversion is a direct result of the multiple copies of the prophage. Preliminary attempts to identify lambda genes that may be responsible for conversion ruled out several candidates, implicating a potentially novel lambda function that awaits further studies.

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Section: Summary and Discussionmentioning

confidence: 99%