Bacteriophage potential against Vibrio parahaemolyticus biofilms

Yin, Yujie; Ni, Pei’en; Liu, Danlei; Yang, Shiqiang; Almeida, Adelaide; Quan-you, Guo; Zhang, Zilei; Deng, Liuyan; Wang, D.

doi:10.1016/j.foodcont.2018.11.034

Cited by 43 publications

(28 citation statements)

References 28 publications

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“…Although phage FM10 proved to be able to lyse all the STEC strains used in these study, we decided to use a cocktail of different bacteriophages to be more effective and reduce the emergence of phage resistance (Goodridge and Abedon, 2003). The choice of using three phages seemed, in accordance with literature (O'Flynn et al, 2004;Bai et al, 2019;Yin et al, 2019) a good compromise considering the possibility of phage recombination and the generation of new host specificity, and even the high production costs. Besides, according to the "Red Queen hypothesis" ("It takes all the running you can do, to keep in the same place"), as bacteria develop phage defense mechanisms for their survival, phages continuously adapt to these altered host systems in order to avoid a complete destruction (Lythgoe and Read, 1998).…”

Section: Discussionsupporting

confidence: 52%

Evaluation of a Potential Bacteriophage Cocktail for the Control of Shiga-Toxin Producing Escherichia coli in Food

et al. 2020

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Shiga-toxin producing Escherichia coli (STEC) are important foodborne pathogens involved in gastrointestinal diseases. Furthermore, the recurrent use of antibiotics to treat different bacterial infections in animals has increased the spread of antibiotic-resistant bacteria, including E. coli, in foods of animal origin. The use of bacteriophages for the control of these microorganisms is therefore regarded as a valid alternative, especially considering the numerous advantages (high specificity, self-replicating, self-limiting, harmless to humans, animals, and plants). This study aimed to isolate bacteriophages active on STEC strains and to set up a suspension of viral particles that can be potentially used to control STEC food contamination. Thirty-one STEC of different serogroups (O26; O157; O111; O113; O145; O23, O76, O86, O91, O103, O104, O121, O128, and O139) were investigated for their antibiotic resistance profile and sensitivity to phage attack. Ten percent of strains exhibited a high multi-resistance profile, whereas ampicillin was the most effective antibiotic by inhibiting 65% of tested bacteria. On the other side, a total of 20 phages were isolated from feces, sewage, and bedding material of cattle. The viral particles proved not to carry genes codifying Shiga-toxins and intimin. No STEC was resistant to all phages, although some strains revealed weak sensitivity by forming turbid plaques. Three different bacteriophages (forming the "cocktail") were selected considering their different RAPD (Random Amplification of Polymorphic DNA) profiles and the absence of virulence-encoding genes and antibiotic-resistance genes. The lytic ability against STEC strains was investigated at different multiplicity of infection (MOI = 0.1, 1, and 10). Significant differences (p < 0.05) among mean values of optical density were observed by comparing results of experiments at different MOI and controls. An effective reduction of bacterial population was obtained in 81% of cases, with top performance when the highest MOI was applied. The efficacy of the phage cocktail was tested on fresh cucumbers. Results showed a reduction in pathogenic E. coli by 1.97-2.01 log CFU/g at 25 • C and by 1.16-2.01 log CFU/g at 4 • C during 24 h, suggesting that the formulated cocktail could have the potential to be used in bio controlling STEC different serogroups.

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Section: Discussionsupporting

confidence: 52%

Evaluation of a Potential Bacteriophage Cocktail for the Control of Shiga-Toxin Producing Escherichia coli in Food

et al. 2020

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“…This explains the host specificity of the phages isolated in this study. External factors such as pH and temperature may influence the stability and infectivity of the phages (7). These factors may fluctuate, particularly in live animals because of diet and/or ambient temperature.…”

Section: Discussionmentioning

confidence: 99%

“…They are found in various environments related to their host such as in food, soil, sewage water, feces, and farm environments (2). Several bacterial species such as Campylobacter, Escherichia coli, Listeria, Salmonella, Pseudomonas, and Vibrio species are used as hosts to isolate their specific bacteriophages (5)(6)(7). Because of their host specificity and nontoxicity, lytic phages are considered to be an alternative solution to combat antimicrobial-resistant pathogens.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Lytic Bacteriophages Infecting Multidrug-Resistant Shiga Toxigenic Atypical Escherichia coli O177 Strains Isolated From Cattle Feces

Montso

Mlambo

Ateba

2019

Front. Public Health

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The increasing incidence of antibiotic resistance and emergence of virulent bacterial pathogens, coupled with a lack of new effective antibiotics, has reignited interest in the use of lytic bacteriophage therapy. The aim of this study was to characterize lytic Escherichia coli O177-specific bacteriophages isolated from cattle feces to determine their potential application as biocontrol agents. A total of 31 lytic E. coli O177-specific bacteriophages were isolated. A large proportion (71%) of these phage isolates produced large plaques while 29% produced small plaques on 0.3% soft agar. Based on different plaque morphologies and clarity and size of plaques, eight phages were selected for further analyses. Spot test and efficiency of plating (EOP) analyses were performed to determine the host range for selected phages. Phage morphotype and growth were analyzed using transmission electron microscopy and the one-step growth curve method. Phages were also assessed for thermal and pH stability. The spot test revealed that all selected phages were capable of infecting different environmental E. coli strains. However, none of the phages infected American Type Culture Collection (ATCC) and environmental Salmonella strains. Furthermore, EOP analysis (range: 0.1-1.0) showed that phages were capable of infecting a wide range of E. coli isolates. Selected phage isolates had a similar morphotype (an icosahedral head and a contractile tail) and were classified under the order Caudovirales, Myoviridae family. The icosahedral heads ranged from 81.2 to 110.77 nm, while the contractile tails ranged from 115.55 to 132.57 nm in size. The phages were found to be still active after 60 min of incubation at 37 and 40 • C. Incremental levels of pH induced a quadratic response on stability of all phages. The pH optima for all eight phages ranged between 7.6 and 8.0, while at pH 3.0 all phages were inactive. Phage latent period ranged between 15 and 25 min while burst size ranged from 91 to 522 virion particles [plaque-forming unit (PFU)] per infected cell. These results demonstrate that lytic E. coli O177-specific bacteriophages isolated from cattle feces are highly stable and have the capacity to infect different E. coli strains, traits that make them potential biocontrol agents.

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“…Conventional Psa control agents are being faced with the emergence of resistant Psa strains, and cause environmental contamination. The application of phages in agriculture has received great attention in recent years, because phages exhibit specific infectivity toward the targeted bacterium, and have no direct negative effects on animals or plants [30][31][32]. Bae et al used a lytic phage to control bacterial wilt in tomato [33] and Czajkowski et al isolated phages from soil to control Dickeya spp., which causes potato blackleg and soft rot in potato tubers [34], resulting in the efficient inactivation of the pathogenic bacteria without affecting the plants.…”

Section: Discussionmentioning

confidence: 99%

Combined Application of Bacteriophages and Carvacrol in the Control of Pseudomonas syringae pv. actinidiae Planktonic and Biofilm Forms

Wang

Deng

et al. 2020

Microorganisms

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Pseudomonas syringae pv. actinidiae (Psa) is the causative agent of the bacterial canker of kiwifruit (Actinidia spp.). Phage therapy has been suggested as a viable alternative approach to controlling this disease, but its efficacy is limited by the emergence of phage-resistant mutants. Carvacrol is an essential oil that may be useful for the control of Psa. Combination therapies can be used to overcome resistance development. Here, the combination of phages (single phage suspensions of phages PN05 and PN09, and a cocktail of both phages) and carvacrol was investigated in controlling Psa planktonic and biofilm forms in vitro. The phage therapy alone (with phages PN05 and PN09), and the carvacrol alone (minimum inhibitory concentration 2.0 mg/mL), inhibited Psa growth, but the combined effect of both therapies was more effective. The phages alone effectively inhibited Psa growth for 24 h, but Psa regrowth was observed after this time. The carvacrol (2.0 mg/mL) alone prevented the biofilm formation for 48 h, but did not destroy the pre-formed biofilms. The combined treatment, phages and carvacrol (2.0 mg/mL), showed a higher efficacy, preventing Psa regrowth for more than 40 h. In conclusion, the combined treatment with phages and carvacrol may be a promising, environment-friendly and cost-effective approach to controlling Psa in the kiwifruit industry.

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Bacteriophage potential against Vibrio parahaemolyticus biofilms

Cited by 43 publications

References 28 publications

Evaluation of a Potential Bacteriophage Cocktail for the Control of Shiga-Toxin Producing Escherichia coli in Food

Evaluation of a Potential Bacteriophage Cocktail for the Control of Shiga-Toxin Producing Escherichia coli in Food

Characterization of Lytic Bacteriophages Infecting Multidrug-Resistant Shiga Toxigenic Atypical Escherichia coli O177 Strains Isolated From Cattle Feces

Combined Application of Bacteriophages and Carvacrol in the Control of Pseudomonas syringae pv. actinidiae Planktonic and Biofilm Forms

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