Bacteriophage T1

Drexler, Henry

doi:10.1007/978-1-4684-5424-6_7

Cited by 11 publications

(8 citation statements)

References 85 publications

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“…; Fig 1 and S5 Table ), we included a panel of 10 classical model phages in our genomic and phenotypic characterization and view them as an accessory part of the BASEL collection. Beyond the T phages (except T1 that is a notorious laboratory contaminant [ 30 ]), we included well-studied podovirus N4 and obligately lytic mutants of the 3 most commonly studied temperate phages lambda, P1, and P2 [ 5 , 7 , 31 , 32 ] ( Fig 1 ; see also S5 Table ).…”

Section: Resultsmentioning

confidence: 99%

Systematic exploration of Escherichia coli phage–host interactions with the BASEL phage collection

et al. 2021

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Bacteriophages, the viruses infecting bacteria, hold great potential for the treatment of multidrug-resistant bacterial infections and other applications due to their unparalleled diversity and recent breakthroughs in their genetic engineering. However, fundamental knowledge of the molecular mechanisms underlying phage–host interactions is mostly confined to a few traditional model systems and did not keep pace with the recent massive expansion of the field. The true potential of molecular biology encoded by these viruses has therefore remained largely untapped, and phages for therapy or other applications are often still selected empirically. We therefore sought to promote a systematic exploration of phage–host interactions by composing a well-assorted library of 68 newly isolated phages infecting the model organism Escherichia coli that we share with the community as the BASEL (BActeriophage SElection for your Laboratory) collection. This collection is largely representative of natural E. coli phage diversity and was intensively characterized phenotypically and genomically alongside 10 well-studied traditional model phages. We experimentally determined essential host receptors of all phages, quantified their sensitivity to 11 defense systems across different layers of bacterial immunity, and matched these results to the phages’ host range across a panel of pathogenic enterobacterial strains. Clear patterns in the distribution of phage phenotypes and genomic features highlighted systematic differences in the potency of different immunity systems and suggested the molecular basis of receptor specificity in several phage groups. Our results also indicate strong trade-offs between fitness traits like broad host recognition and resistance to bacterial immunity that might drive the divergent adaptation of different phage groups to specific ecological niches. We envision that the BASEL collection will inspire future work exploring the biology of bacteriophages and their hosts by facilitating the discovery of underlying molecular mechanisms as the basis for an effective translation into biotechnology or therapeutic applications.

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Section: Resultsmentioning

confidence: 99%

Systematic exploration of Escherichia coli phage–host interactions with the BASEL phage collection

et al. 2021

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“…T1-like phages have an icosahedral head of about 60 nm and a slender tail of about 150 nm, placing them in the newly formed family of tailed phages, Drexlerviridae , which consists of T1-like phages from the Siphoviridae family ( German et al, 2006 ; Adriaenssens et al, 2020 ). Phage T1 itself was one of the first phages to be studied in detail as it was one of the original “type” phages selected by Drexler (1988) . Phage T1 was shown to have unique properties and soon became infamous for its ability to invade microbiological laboratories, ruining many experiments by lysing the standard Escherichia coli strain K12.…”

Section: Introductionmentioning

confidence: 99%

“…T1-like phages are virulent and have high adsorption rates, and as such have gained attention as candidate phages in the development of phage therapy ( Kutter et al, 2010 ; Abedon et al, 2011 ; Divya Ganeshan and Hosseinidoust, 2019 ). The adsorption rate of phage T1 is around 3 × 10 –9 mL × min –1 , which is considered to be among the highest rates of phage adsorption, and the time from infection to lysis of the bacterium is short, 15–20 min ( Drexler, 1988 ; German et al, 2006 ). These properties would prove advantageous for phage therapy as they theoretically lead to a fast elimination of an infecting bacteria, provided that the phage dose reaching the bacteria is high enough.…”

Section: Introductionmentioning

confidence: 99%

Infection Kinetics and Phylogenetic Analysis of vB_EcoD_SU57, a Virulent T1-Like Drexlerviridae Coliphage

et al. 2020

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“…Besides these 66 Table), we included a panel of ten classical model phages in our genomic and phenotypic characterization and view them as an accessory part of the BASEL collection. Beyond the T phages (without T1 that is a notorious laboratory contaminant [32]), we included well-studied podovirus N4 and obligately lytic mutants of the three most commonly studied temperate phages lambda, P1, and P2 [5,7,33,34] (Fig 2; see also S5 Table).…”

Section: Composition Of the Basel Collectionmentioning

confidence: 99%

“…In addition to the 66 newly isolated bacteriophages, ten classical model phages were included for genomic and phenotypic characterization, and we view these phages as an accessory part of the BASEL collection. These ten phages were six of the seven T phages (excluding T1 because it is a notorious laboratory contaminant [32]), phage N4, and obligately lytic mutants of the three well-studied temperate phages lambda, P1, and P2 [5,7,33,34] (Fig 2; see also S5 Table ). To generate the T3(K12) chimera, the 3' end of gp17 lateral tail fiber gene of phage T7 was cloned into lowcopy plasmid pUA139 with flanking regions exhibiting high sequence similarity to the phage T3 genome (generating pUA139_T7(gp17), see S4 Table ).…”

Section: And S5 Table For Details) Phagesmentioning

confidence: 99%

Systematic exploration ofEscherichia coliphage-host interactions with the BASEL phage collection

Maffei

Shaidullina

Burkolter

et al. 2021

Preprint

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Bacteriophages, the viruses infecting bacteria, hold great potential for the treatment of multidrug-resistant bacterial infections and other applications due to their unparalleled diversity and recent breakthroughs in their genetic engineering. However, fundamental knowledge of molecular mechanisms underlying phage-host interactions is mostly confined to a few traditional model systems and did not keep pace with the recent massive expansion of the field. The true potential of molecular biology encoded by these viruses has therefore remained largely untapped, and phages for therapy or other applications are often still selected empirically. We therefore sought to promote a systematic exploration of phage-host interactions by composing a well-assorted library of 66 newly isolated phages infecting the model organism Escherichia coli that we share with the community as the BASEL collection (BActeriophage SElection for your Laboratory). This collection is largely representative of natural E. coli phage diversity and was intensively characterized phenotypically and genomically alongside ten well-studied traditional model phages. We experimentally determined essential host receptors of all phages, quantified their sensitivity to eleven defense systems across different layers of bacterial immunity, and matched these results to the phages' host range across a panel of pathogenic enterobacterial strains. Our results reveal clear patterns in the distribution of phage phenotypes and genomic features that highlight systematic differences in the potency of different immunity systems and point towards the molecular basis of receptor specificity in several phage groups. Strong trade-offs were detected between fitness traits like broad host recognition and resistance to bacterial immunity that might drive the divergent adaptation of different phage groups to specific niches. We envision that the BASEL collection will inspire future work exploring the biology of bacteriophages and their hosts by facilitating the discovery of underlying molecular mechanisms as the basis for an effective translation into biotechnology or therapeutic applications.

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Bacteriophage T1

Cited by 11 publications

References 85 publications

Systematic exploration of Escherichia coli phage–host interactions with the BASEL phage collection

Systematic exploration of Escherichia coli phage–host interactions with the BASEL phage collection

Infection Kinetics and Phylogenetic Analysis of vB_EcoD_SU57, a Virulent T1-Like Drexlerviridae Coliphage

Systematic exploration ofEscherichia coliphage-host interactions with the BASEL phage collection

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