Bacteriophage T3 and bacteriophage T7 virus-host cell interactions.

Krüger, Detlev H.; Schroeder, Cornelia

doi:10.1128/mmbr.45.1.9-51.1981

Cited by 77 publications

(32 citation statements)

References 312 publications

(524 reference statements)

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“…An interesting parallel is found in coliphage T7, in which selection has removed recognition sequences for many E. coli-based restriction systems, with the exception of EcoPI. The EcoPI cut site overlaps the T7 primase binding sequence, illustrating preservation of function outweighing selection pressure (22,33). An alternative explanation for the presence of AT-rich palindromes in HF2 is that there may be few halobacterial restriction systems that target AT-rich DNA, a reflection of the GC-rich nature of halobacterial DNA.…”

Section: Discussionmentioning

confidence: 99%

Halophage HF2: genome organization and replication strategy

Nuttall

Dyall‐Smith

1995

J Virol

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Halophage HF2 is a lytic, broad-host-range bacteriophage of the extremely halophilic domain Archaea. It has a 79.7-kb double-stranded DNA genome which is linear, contains no modified nucleotides, and is not susceptible to cleavage by many type II restriction endonucleases. This insensitivity is attributed to selection against palindromic restriction sites, a commonly observed feature of broad-host-range phages. Interestingly, enzymes that did cut the genome recognized AT-rich sites, and five such enzymes, DraI, AseI, HpaI, HindIII, and SspI, were used to construct a physical map of the genome. Southern hybridization experiments used to order fragments on the map indicated homologies between the phage termini, and subsequent sequence analysis showed that HF2 possessed 306-bp direct terminal repeats. The presence of such repeats suggested replication through concatameric intermediates, and this was confirmed by analysis of the state of the phage genome in infected cells. This is a replication strategy adopted by many well-studied bacterial phages, for example T3 and T7. Other similarities between the terminal repeats of T3 or T7 and HF2 include a putative nick site at the repeat border and a series of short imperfect repeats. These observations suggest a long evolutionary history for concatamer-based strategies of phage replication, possibly predating the divergence of Archaea/Eucarya and Bacteria, or alternatively, indicate possible lateral transfer of phage genes or modules between the domains Archaea and Bacteria.

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Section: Discussionmentioning

confidence: 99%

Halophage HF2: genome organization and replication strategy

Nuttall

Dyall‐Smith

1995

J Virol

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“…Since the original description of phage-coded activities that prevent T7 and T4 restriction (10,26), this phenomenon has been reported for T3, T5, and bacillus phage NR2 (for review, see reference 17). More recently, the direct interaction of the T7 antirestriction protein with the host-restricting enzyme has been nicely demonstrated (19).…”

Section: Discussionmentioning

confidence: 89%

Physical mapping and cloning of bacteriophage T4 anti-restriction endonuclease gene

Dharmalingam¹,

Revel²,

Goldberg³

1982

J Bacteriol

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“…Bacteriophage T7 is an attractive system for the study of DNA metabolism and genetic regulation. This phage has been extensively characterized genetically (4,22,23), and its genome consists of a double-stranded DNA molecule which codes for about 50 proteins. The entire genomic DNA molecule has recently been sequenced (J. Dunn and F. W. Studier, J. Mol.…”

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confidence: 99%

“…Genetic recombination of T7 has been studied in vivo (3, 5, 7-9, 19, 20, 24, 25). From these studies, it was concluded that T7 recombination in vivo requires the products of T7 gene 3 (endonuclease), gene 4 (DNA primase), gene 5 (DNA polymerase), and gene 6 (exonuclease). Recombination is independent of the host and phage ligase, DNA packaging, and maturation functions.…”

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confidence: 99%

In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay

Lee¹,

Sadowski²

1983

J Virol

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We developed a simple, direct, physical assay to detect genetic recombination of bacteriophage T7 DNA in vitro. In this assay two mature T7 DNA molecules, each having a unique restriction enzyme site, are incubated in the presence of a cell-free extract from T7-infected Escherichia coli cells. After extraction of the DNA, restriction enzyme digestion, and agarose gel electrophoresis, genetic recombination is detected by the appearance of a novel recombinant DNA band. Recombination frequencies as high as 13% have been observed. We used this assay to determine the genetic requirements for in vitro recombination. In agreement with results obtained previously with a biological assay, T7 recombination in vitro appears to proceed via two distinct pathways.

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Bacteriophage T3 and bacteriophage T7 virus-host cell interactions.

Abstract: Restriction Analysis of Phage Deoxyribonucleic Acid (DNA) The utility of sequence-specific restriction endonucleases for physical mapping and sequence analysis of genomes and for gene cloning (272, 327, 328, 394, 395, 466) has had its impact also MICROBIOL. REV.

Cited by 77 publications

References 312 publications

Halophage HF2: genome organization and replication strategy

Halophage HF2: genome organization and replication strategy

Physical mapping and cloning of bacteriophage T4 anti-restriction endonuclease gene

In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay

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