Bacteriophage T5 and Related Phages

McCorquodale, D.J.; Warner, Huber R.

doi:10.1007/978-1-4684-5424-6_10

Cited by 41 publications

(31 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of these genes encode structural proteins and appear to be clustered within two regions. The left region contains genes encoding tail proteins and the right region contains genes encoding head protein (41). The oad gene, encoding the host receptor binding protein, makes an exception, since it is located to the right of genes N4, N5, and D20-21, encoding head proteins (16,19,23,65).…”

Section: Resultsmentioning

confidence: 99%

“…T5 uses the E. coli polymerase in conjunction with the c70 factor for all different classes of transcription; pre-early, early, and late (41). This complex becomes successively modified during pre-early phase by gene products A3 (56) and Al (40) to allow transcription of early genes and during early phase by gene product C2 and a 15K protein (57) to allow transcription of late genes.…”

Section: Resultsmentioning

confidence: 99%

“…This complex becomes successively modified during pre-early phase by gene products A3 (56) and Al (40) to allow transcription of early genes and during early phase by gene product C2 and a 15K protein (57) to allow transcription of late genes. Transcription of late genes, in addition, requires the activity of host DNA gyrase (8) and T5 gene products D5 and D15 (41).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Cloning, sequencing, and recombinational analysis with bacteriophage BF23 of the bacteriophage T5 oad gene encoding the receptor-binding protein

Krauel

Heller

1991

J Bacteriol

View full text Add to dashboard Cite

Binding of bacteriophage T5 to its receptor, the Escherichia coli FhuA protein, is mediated by tail protein pb5. In this article we confirm that pb5 is encoded by the T5 oad gene and describe the isolation, expression, and sequencing of this gene. In order to locate oad precisely, we analyzed recombinants between BF23, a T5-related phage with a different host range, and plasmid clones containing segments of the T5 chromosome. This analysis also showed that oad has little or no homology with hrs, the analogous BF23 gene. We were able to overproduce a protein that comigrates with pb5 after fusing a 2-kb segment containing oad to a phage T7

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Cloning, sequencing, and recombinational analysis with bacteriophage BF23 of the bacteriophage T5 oad gene encoding the receptor-binding protein

Krauel

Heller

1991

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Phage T5 can inhibit the activity of EcoRI (Davison & Brunel, 1979) and a variety of host enzymes that modify DNA (see McCorquodale & Warner, 1988), but most of the anti-restriction functions currently identified are directed against type I systems. It seems unlikely that this bias towards functions that protect against type I systems simply reflects the fact that most work has been done with E. coli K-12 and E. coli B; E. coli strains specifying EcoRI have been in common laboratory use for 30 years.…”

Section: Is Restriction An Effective Barrier To the Acquisition Of Fomentioning

confidence: 99%

Immigration control of DNA in bacteria: self versus non-self

Murray¹

2002

Microbiology

153

143

View full text Add to dashboard Cite

“…2 The capsid contains an approximately 120 kbp double-stranded (ds)DNA genome, the largest of the T-odd group (T1, T3, T5, and T7). 3 Other distinguishing features of T5 are the single-stranded interruptions (nicks) that occur at well-defined positions in its genome 3 and a unique two-step genome transfer mechanism that leads to transcription of pre-early genes before the remaining phage DNA has entered the host cell. 4 Although the T5-like bacteriophages have been classified as a separate genus, recent electron microscopy work has revealed similarities to T4 as well as Lambda-like phages, suggesting unexpected evolutionary relationships.…”

Section: Introductionmentioning

confidence: 99%