Bacteriophage: The Viruses Infecting Bacteria and Their Multiple Applications

Húngaro, Humberto Moreira; Lopez, Maryoris Elisa Soto; Albino, Luiz Augusto Aguiar; Mendonça, Regina Célia Santos

doi:10.1016/b978-0-12-409548-9.09039-4

Cited by 4 publications

(5 citation statements)

References 145 publications

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“…There are basically four types of genetic material comprising bacteriophages genome, single-stranded and double-stranded DNA and RNA (ssDNA, dsDNA, ssRNA, dsRNA) [10,11]. Genetic material varies widely among phages: genome length ranges from 3405 bp to 497513 bp, gene density ranges from 0.29 to 1.36 and number of encoded proteins ranges from 1 to 675.…”

Section: Phage Genome Sequencingmentioning

confidence: 99%

Bacteriophage Genome Sequencing: A New Alternative to Understand Biochemical Interactions between Prokaryotic Cells and Phages

G¹,

B²,

A³

et al. 2017

J Microb Biochem Technol

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Section: Phage Genome Sequencingmentioning

confidence: 99%

Bacteriophage Genome Sequencing: A New Alternative to Understand Biochemical Interactions between Prokaryotic Cells and Phages

G¹,

B²,

A³

et al. 2017

J Microb Biochem Technol

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“…These phages are characterized by icosahedral heads, dsDNA and non-contractile tail. 10 On the other hand, Pa11 phage with specificity for P. fluorescens was included into the Myoviridae family, presented contractile neck 10 and dsDNA. Although having similar morphology, the genetic profile of phages is not obligatorily similar, because the environment of isolation is different and faraway.…”

Section: Phage Morphological Characterizationmentioning

confidence: 99%

“…It is estimated that the number of phages on the planet are higher than 10 30 viral particles. 9,10 Phages are considered ubiquitous agents. 8,10 Like all virus, phages do not have intrinsic metabolism and depend on metabolic machinery of host bacteria for replication.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of Lytic Bacteriophages as an Alternative to Prevent Pseudomonas spp in Poultry Industry

Lopez¹,

Carvalho²,

Boggione³

et al. 2015

MOJFPT

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The Pseudomonas genus is a big problem mainly for the poultry food industry. The shelf life of chicken carcasses stored under refrigeration is limited by the appearance of undesirable odors and sliminess surface generated primarily by Gram negative bacteria. Due to the subsequent emergence of resistant bacteria, is necessary proving new alternatives as guaranty the microbiological quality of foods and human health. Bacteriophages or phages are viruses of bacteria that use resources of bacteria for their replication and killing bacteria "naturally", showing them as a potential tool for bacteria biocontrol in food industry. In this study, 11 bacteriophages were isolated from the exudate product of defrost of chicken carcasses using as host strains Pseudomonas aeruginosa (ATCC 25619) and Pseudomonas fluorescens (ATCC 13525). This study also aimed at the purification, quantification and morphological and molecular characterization of phages (RFLP). Bacteriophage can be found in all types of environments, it was possible to perform isolation and purification and achieve concentrations of 1011PFU/mL, which demonstrated a broad spectrum of action on different bacteria of the family Enterobacteriaceae. Bacteriophages were classified as belonging to the order of Caudovirales and families Podoviridae and Myoviridae. Bacteriophages showed similar morphology, but some showed different genetic profiles.

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“…In this cycle, bacteriophages cause cellular lysis by injecting their genetic material into the cell to create new virus particles (Muñoz and Koskella, 2014) The International Committee on Taxonomy of Viruses (ICTV) identi es the existence of ten families of bacteriophages based on conserved genomic synteny, homology in amino acid sequences of phage genetic material encoded proteins, and capsid morphology (Tolstoy et al, 2018). The bacteriophage genome exists as one of the four possible forms of nucleic acids (ssDNA, dsDNA, ssRNA, and dsRNA) (Nguyen et al, 2023;Hungaro et al, 2014). Phages differ greatly in terms of their genetic makeup: their genome lengths range from 3405 bp to 497513 bp, their gene densities from 0.29 to 1.36, and the number of encoded proteins they contain from 1 to 675.…”

Section: Introductionmentioning

confidence: 99%

Whole genome sequence analysis and characterization of lytic bacteriophages against antimicrobial-resistant diarrheagenic Escherichia coli strains isolated from various sources in Addis Ababa, Ethiopia

Sada,

Alemayehu,

Tafese

et al. 2024

Preprint

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The emergence of antibiotic resistance in E. coli strains has sparked a fervent investigation of alternative therapies such as the use of lytic bacteriophages. Phage whole genome sequencing is a novel method for learning more about proteins and other biomolecules encoded by phages, particularly phage lytic enzymes that are crucial to the lysis of bacterial cells. Seven potential lytic E. coli phages; EH-B-A (A1), EP-M-A, EP-B-K (E2), EI-SP-GF, ET-SD-TH, and ST-TK isolated from activated dairy farm sludges, Rivers, and hospital liquid waste were described. For sequencing, an Illumina NextSeq 550 sequencer was used. The virus nucleotide collection (nr/nt) (taxid:10239) was used to evaluate the whole genome sequences. Phylogenetic analysis was done using MEGA11 software. Genome sequencing revealed that each bacteriophage contains a linear double-stranded DNA genome. Phage isolates were taxonomically identified as 4 (57%) Myoviridae and 3 (43%) Siphoviridae phages. Phage genome length varied from 24264 to 143,710 bp, and their GC contents ranged from 43 to 54%. 33–218 CDSs (coding sequences) in total were predicted, with 19–77% of CDSs encoding functional proteins. All phages lacked tRNA in their genomes, except for EI-SP-GF, which possessed five tRNAs. Based on phylogenetic tree analysis, the phage isolates were related to Enterobacteria and E. coli phage sequences in the database. Screening did not show any genes encoding for a CRISPR-like system, virulence, antibiotic resistance, or lysogeny. Because of their stringent lytic nature, these phage isolates may be applied in the future to treat E. coli infections. This study may provide some primary data for the development of phage control techniques and advance our understanding of the genetic composition of E. coli phages.

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Bacteriophage: The Viruses Infecting Bacteria and Their Multiple Applications

Cited by 4 publications

References 145 publications

Bacteriophage Genome Sequencing: A New Alternative to Understand Biochemical Interactions between Prokaryotic Cells and Phages

Bacteriophage Genome Sequencing: A New Alternative to Understand Biochemical Interactions between Prokaryotic Cells and Phages

Isolation and Characterization of Lytic Bacteriophages as an Alternative to Prevent Pseudomonas spp in Poultry Industry

Whole genome sequence analysis and characterization of lytic bacteriophages against antimicrobial-resistant diarrheagenic Escherichia coli strains isolated from various sources in Addis Ababa, Ethiopia

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