Bacteriophage ФX174 DNA synthesis in Escherichia coli HF4704S (dnaHts) cells

Sugiyama, Hiroshi; Komano, Tohru

doi:10.1016/0005-2787(75)90067-2

Cited by 9 publications

(4 citation statements)

References 29 publications

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“…In our initial experiments with the mutant strain HF4704S we observed temperature-sensitive host DNA synthesis in uninfected cells and temperature-sensitive phage DNA synthesis in OX174-infected cells, consistent with results previously reported (21,22). However, we also observed an unusually high rate of incorporation of [3H]thymidine into bX174 at 30°C in this mutant host when compared with the nondefective parent strain.…”

Section: Resultssupporting

confidence: 91%

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Deoxyribonucleic acid synthesis in a temperature-sensitive Escherichia coli dnaH mutant, strain HF4704S

Derstine¹,

Dumas²

1976

J Bacteriol

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The dnaH mutant strain HF4704S, isolated by Sakai et al. (1974), was examined for its effect on kX174 deoxyribonucleic acid (DNA) synthesis. It was found to carry two mutations affecting DNA synthesis. One mutation had no affect on OX174 DNA synthesis, but did affect the ability of the mutant cells to form colonies on agar medium at 410C, and caused host DNA synthesis to cease after 1 h at 410C. The mutant marker cotransduced with ilvD at a frequency of about 9%. It seems likely that this mutation is in the dnaA gene. The second mutation affected the ability of the mutant cells to form colonies on agar medium supplemented with only 2 ug of thymine per ml, and affected both host and OX174 DNA synthesis in medium supplemented with only 2 ug of thymine per ml. Both effects could be overcome by adding excess exogenous thymine. We were not able to unambiguously determine the map position of this mutant locus. Our data show that the DNA synthesis phenotype of the mutant strain HF4704S is governed by both of these mutations, neither of which directly affects the replication of 4X174 DNA.

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Section: Resultssupporting

confidence: 91%

“…Others have reported (21,22) that E. coli strain HF4704S carries a temperature-sensitive mutation in the dnaH gene which causes temperature-sensitive OX174 DNA synthesis in medium containing 2 pAg of thymine per ml, and causes temperature-sensitive initiation of host DNA synthesis. They also reported that the mutant locus is closely linked to thyA.…”

Section: Cmentioning

confidence: 99%

Deoxyribonucleic acid synthesis in a temperature-sensitive Escherichia coli dnaH mutant, strain HF4704S

Derstine¹,

Dumas²

1976

J Bacteriol

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“…Phage DNA synthesis was estimated by the incorporation of [3H]thymidine into acid-insoluble material. The procedures were essentially the same as those reported by Sakai and Komazo (20).…”

Section: Methodsmentioning

confidence: 96%

“…Media and buffers. TPG-CA medium was described by Sakai and Komano (20), and starvation V ,X174 GROWTH IN A dnaP MUTANT 651 buffer (SB) was described by Denhardt and Sinsheimer (3). Borate buffer was 0.05 M sodium tetraborate solution, pH 9.5.…”

Section: Methodsmentioning

confidence: 99%

Growth and DNA synthesis of bacteriophage phi x174 in a dnaP mutant of Escherichia coli

Mano¹,

Sakai²,

Komano³

1979

J Virol

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4)X174am3trD, a temperature-resistant mutant of bacteriophage 4X174am3, exhibited a reduced ability to grow in a dnaP mutant, Escherichia coli KM107, at the restrictive temperature (430C). Under conditions at which the dnaP gene function was inactivated, the amount and the rate of 4X174am3trD DNA synthesis were reduced. The efficiency of phage attachment to E. coli KM107 at 43°C was the same as to the parental strain, E. coli KD4301, but phage eclipse and phage DNA penetration were inhibited in E. coli KM107 at 430C. It is suggested that the dnaP gene product, which is necessary for the initiation of host DNA replication, participates in the conversion of attached phages to eclipsed particles and in phage DNA penetration in vivo in normal infection.

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Thymidine Metabolism in Bacteria (and “How, or How Not, to Label DNA”)

O’Donovan

1978

DNA Synthesis

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Bacteriophage ФX174 DNA synthesis in Escherichia coli HF4704S (dnaHts) cells

Cited by 9 publications

References 29 publications

Deoxyribonucleic acid synthesis in a temperature-sensitive Escherichia coli dnaH mutant, strain HF4704S

Deoxyribonucleic acid synthesis in a temperature-sensitive Escherichia coli dnaH mutant, strain HF4704S

Growth and DNA synthesis of bacteriophage phi x174 in a dnaP mutant of Escherichia coli

Thymidine Metabolism in Bacteria (and “How, or How Not, to Label DNA”)

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