Bacteriophages for the control of Klebsiella outbreak in the neonatal intensive care unit

Introduction. Currently, there is insufficient data on the prevalence of Klebsiella pneumoniae strains with virulence factors genes uge and fim among women and newborns. This indicates the need for a study of the prevalence of K. pneumoniae (uge+, fim+) and the degree of heterogeneity of the bacterial population isolated from children and adults.The aim of the study was to perform a phylogenetic analysis of the uge and fim genes of the K. pneumoniae strains.Materials and methods. Total 65 strains of K. pneumoniae isolated from samples of feces, blood, urine, placenta, cervical canal, pharynx, suture of 39 newborns and 24 women were studied. Two blood cultures were obtained from one patient with an interval of two weeks, and two isolates were obtained from the separated cervical canal and suture of one patient. The presence of genes was detected by PCR, nucleotide sequences of the genes were determined by Sanger sequencing.Results. The frequency of detection of the uge gene was 53.8% (35 of 65), fim gene — 23.1% (15 of 65), which indicates a higher prevalence of uge gene strains compared to fim (p < 0.001). The phylogenetic analysis of 18 nucleotide sequences of the uge gene and 4 of the fim gene demonstrated that the strains were distributed in 7 and 4 clusters, respectively. It was established that for, there are No clear clustering by time and place of isolation, patient age, and type of biological material was observed for both uge and fim genes.Discussion. The results of phylogenetic analysis demonstrate the genetic heterogeneity of the studied population of K. pneumoniae, which is confirmed by the wide geography and time variations in detection of the most genetically close bacterial isolates.

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The relatedness of Klebsiella pneumoniae strains based on phylogenetic analysis of uge and fim genes

Ustyuzhanin¹,

Чистякова²,

Ремизова³

2021

Journal of microbiology, epidemiology and immunobiology

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Bacteriophage Therapy Against Antimicrobial Resistant Crisis

Ullah,

Ahmad,

Khan

et al. 2023

J. Health. Science. Med. Therapy.

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The most common virus on the earth is bacteriophage (or phages) that are present in all organisms. Their classification is currently being evaluated based on the phage's unique and antibacterial properties. The phage replicates within the host through a lytic or lysogenic process following infection and use of a bacterial cell machine. Phage has become an effective therapeutic drug against pathogens after twort and Filex d'Herelle discovery of bacteriophage in the 1900s, and subsequent research has been conducted. Nevertheless, bacteriophage therapy has become an unavoidable option for research due to the recent occurrence of bacterial antibiotics resistance. Around fifty years after antibiotic were found, antibiotics resistance is key risk for health care. Antimicrobial resistance is a rising big issue in global healthcare. The WHO, 1st report on antimicrobial resistances globally, has emphasized the threat of a forthcoming post antibiotics age, where little infection could be not treatable and once again will be fatal. Considering the present condition, producing therapeutic agent that are complementary to antibiotics play great role to fight against antibiotic resistance. The crisis requires development and implementation of new therapeutic agents against infections and phage therapy is suitable to control infectious diseases because safety of phage therapy. There is a perception with regards to phage therapy that phages are usually safe, on the bases of fact that they are ubiquitous in nature and our continued contact to phages in the environment and furthermore that they are widely used without adverse effects in many of the world. with this positive interpretation, the application of phage therapy must be verified by current research studies. bacteriophage preparations contain detrimental substances, such as toxins of gram-negative bacteria, during the formulation process of bacteriophage and that can be remove by different purification methods. Phages effects normal flora GIT negligibly due to specificity in nature and they infect only a small number of bacterial species.

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Bacteriophages for the control of Klebsiella outbreak in the neonatal intensive care unit

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The relatedness of <i>Klebsiella pneumoniae</i> strains based on phylogenetic analysis of <i>uge</i> and <i>fim</i> genes

The relatedness of <i>Klebsiella pneumoniae</i> strains based on phylogenetic analysis of <i>uge</i> and <i>fim</i> genes

Bacteriophage Therapy Against Antimicrobial Resistant Crisis

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