Bacteriophages LSA2308 and LSA2366 infecting drug-resistant Staphylococcus aureus: Isolation, characterization and potential application for milk safety

Ma, Fei; Yu, Ning; Wan, Qingqing; Zou, Likou; Liu, Yuntao; Chen, Shujuan; Li, Jianlong; Zeng, Zhen; Yang, Yong; Chen, Hong; Zhou, Min; Liu, Shuliang; Li, Cheng; Liu, Aiping

doi:10.1016/j.lwt.2021.112298

Cited by 15 publications

(6 citation statements)

References 41 publications

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“…The activity of VL10 was inactive when the temperature exceeded 50 °C or under extremely basic or acidic pH. These results align with a previous report that demonstrated how LSA2308 exhibited a rapid decrease in its activity when exposed to a temperature of 50 °C 32 . These conditions result in the denaturation of phage proteins and, subsequently, a loss of their viability 41 .…”

Section: Discussionsupporting

confidence: 92%

“…The genome analysis of VL10 revealed its belonging to the Silviavirus genus, known for its effective anti- S. aureus phages with the broadest activity spectrum and display suitable properties for phage therapy application 13 , 14 , 17 . The gene products predicted for VL10 closely resemble those found in other phages within the Silviavirus genus 14 , 17 , 32 and lacks genes for bacterial virulence or antibiotic resistance, making it a promising candidate for therapy. Additionally, within VL10 genome, ORFs58 and 59 encode lysins, which can break down peptidoglycan in bacterial cell walls, causing the bacterial cell to be lysed or destroyed 33 .…”

Section: Discussionmentioning

confidence: 89%

“…However, VL10 demonstrates a comparatively higher burst size than ΦMR003 17 , which typically yields an average burst size of 35 PFU per cell. Another Silviavirus , phage LSA2308 32 , exhibits a shorter latent period (20 min) and a larger burst size (467 PFU) than VL10. These findings underscore the unique growth dynamics of VL10, highlighting both its similarities and distinctions compared to other phages within the Silviavirus genus.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and characterisation of a novel Silviavirus bacteriophage promising antimicrobial agent against methicillin-resistant Staphylococcus aureus infections

Lerdsittikul,

Apiratwarrasakul,

Atithep

et al. 2024

Sci Rep

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The increasing prevalence of methicillin-resistant Staphylococcus aureus (MRSA) emphasises the urgent need for novel antimicrobial agents as alternatives to antibiotics. Bacteriophage therapy is one of the most promising antimicrobial strategies. Here, we isolated and comprehensively characterized a novel Staphylococcus phage, vB_SauM_VL10 (VL10), from urban sewage. The VL10 genome displays 141,746 bp of linear double-stranded DNA, containing 193 open reading frames and lacking tRNA, virulence, or antibiotic resistance genes. Phylogenetic analysis categorizes VL10 as a novel species within the Silviavirus genus, Twortvirinae subfamily. VL10 exhibits lytic behaviour characterized by efficient adsorption, a short latent period, and substantial burst size, with environmental stability. It demonstrates lytic activity against 79.06% of tested S. aureus strains, highlighting its species specificity. Additionally, VL10 effectively targets MRSA biofilms, reducing biomass and viable cells. In MRSA-infected G. mellonella larvae, VL10 enhances survival rates, supporting its potential for phage therapy applications. Moreover, the emergence of VL10-resistant S. aureus strains associated with fitness trade-offs, including reduced growth, biofilm formation, and virulence. Altogether, these findings emphasize VL10 as a promising candidate for developing therapeutic agents against MRSA infections, providing insights into phage biology and resistance dynamics.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Isolation and characterisation of a novel Silviavirus bacteriophage promising antimicrobial agent against methicillin-resistant Staphylococcus aureus infections

Lerdsittikul,

Apiratwarrasakul,

Atithep

et al. 2024

Sci Rep

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“…Various research effects on phage application methods have established phage therapy as a promising alternative treatment. Multiple studies have shown that isolated phages from different environmental sources exhibit high specificity towards specific bacterial strains, particularly against multidrug S. aureus [114][115][116][117][118]. Phage cocktails, composed of lytic bacteriophages individually targeting specific pathogenic strains, have shown significant results, influencing their administration [119][120][121][122][123].…”

Section: Exploring the Potential Of Phage Therapy In Modern Medicinementioning

confidence: 99%

Emerging strategies and therapeutic innovations for combating drug resistance in Staphylococcus aureus strains: A comprehensive review

Gopikrishnan,

Haryini,

2024

J Basic Microbiol

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In recent years, antibiotic therapy has encountered significant challenges due to the rapid emergence of multidrug resistance among bacteria responsible for life‐threatening illnesses, creating uncertainty about the future management of infectious diseases. The escalation of antimicrobial resistance in the post‐COVID era compared to the pre‐COVID era has raised global concern. The prevalence of nosocomial‐related infections, especially outbreaks of drug‐resistant strains of Staphylococcus aureus, have been reported worldwide, with India being a notable hotspot for such occurrences. Various virulence factors and mutations characterize nosocomial infections involving S. aureus. The lack of proper alternative treatments leading to increased drug resistance emphasizes the need to investigate and examine recent research to combat future pandemics. In the current genomics era, the application of advanced technologies such as next‐generation sequencing (NGS), machine learning (ML), and quantum computing (QC) for genomic analysis and resistance prediction has significantly increased the pace of diagnosing drug‐resistant pathogens and insights into genetic intricacies. Despite prompt diagnosis, the elimination of drug‐resistant infections remains unattainable in the absence of effective alternative therapies. Researchers are exploring various alternative therapeutic approaches, including phage therapy, antimicrobial peptides, photodynamic therapy, vaccines, host‐directed therapies, and more. The proposed review mainly focuses on the resistance journey of S. aureus over the past decade, detailing its resistance mechanisms, prevalence in the subcontinent, innovations in rapid diagnosis of the drug‐resistant strains, including the applicants of NGS and ML application along with QC, it helps to design alternative novel therapeutics approaches against S. aureus infection.

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“…However, these methods suffer from severe major weaknesses (Minh et al, 2020). Use of antibiotics in foods is not permitted, whereas chemical agents may have adverse effects due to remaining chemical residues (Bacanli and Başaran, 2019;Ma et al, 2021). Importantly, antibiotics used for agricultural food products such as beef and milk can transmit to humans through the consumption of these foods (Zhan et al, 2018;Bacanli and Başaran, 2019).…”

Section: Introductionmentioning

confidence: 99%

Application of a novel lytic phage vB_EcoM_SQ17 for the biocontrol of Enterohemorrhagic Escherichia coli O157:H7 and Enterotoxigenic E. coli in food matrices

et al. 2022

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Enterohemorrhagic Escherichia coli (EHEC) O157:H7 and Enterotoxigenic E. coli (ETEC) are important foodborne pathogens, causing serious food poisoning outbreaks worldwide. Bacteriophages, as novel antibacterial agents, have been increasingly exploited to control foodborne pathogens. In this study, a novel broad-host range lytic phage vB_EcoM_SQ17 (SQ17), was isolated, characterized, and evaluated for its potential to control bacterial counts in vitro and in three different food matrices (milk, raw beef, and fresh lettuce). Phage SQ17 was capable of infecting EHEC O157:H7, ETEC, and other E. coli strains. Morphology, one-step growth, and stability assay showed that phage SQ17 belongs to the Caudovirales order, Myoviridae family, and Mosigvirus genus. It has a short latent period of 10 min, a burst size of 71 PFU/infected cell, high stability between pH 4 to 12 as well as thermostability between 30°C and 60°C for 60 min. Genome sequencing analysis revealed that the genome of SQ17 does not contain any genes associated with antibiotic resistance, toxins, lysogeny, or virulence factors, indicating the potential safe application of phage SQ17 in the food industry. In Luria-Bertani (LB) medium, phage SQ17 significantly decreased the viable counts of EHEC O157:H7 by more than 2.40 log CFU/ml (p < 0.05) after 6 h of incubation at 37°C. Phage SQ17 showed great potential to be applied for biocontrol of EHEC O157:H7 in milk and raw beef. In fresh lettuce, treatment with SQ17 also resulted in significant reduction of viable cell counts of EHEC O157:H7 and ETEC at both 4°C and 25°C. Our results demonstrate that SQ17 is a good candidate for application as an EHEC O157:H7 and ETEC biocontrol agent in the processing stages of food production and food preservation.

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Bacteriophages LSA2308 and LSA2366 infecting drug-resistant Staphylococcus aureus: Isolation, characterization and potential application for milk safety

Cited by 15 publications

References 41 publications

Isolation and characterisation of a novel Silviavirus bacteriophage promising antimicrobial agent against methicillin-resistant Staphylococcus aureus infections

Isolation and characterisation of a novel Silviavirus bacteriophage promising antimicrobial agent against methicillin-resistant Staphylococcus aureus infections

Emerging strategies and therapeutic innovations for combating drug resistance in Staphylococcus aureus strains: A comprehensive review

Application of a novel lytic phage vB_EcoM_SQ17 for the biocontrol of Enterohemorrhagic Escherichia coli O157:H7 and Enterotoxigenic E. coli in food matrices

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