Bacteriophages of Ralstonia solanacearum: Their Diversity and Utilization as Biocontrol Agents in Agriculture

Yamada, Takashi

doi:10.5772/33983

Cited by 21 publications

(20 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…No filamentous phages (inoviruses) were detected in this study, in contrast to previous studies on soil samples collected in Japan (Yamada et al 2007;Yamada 2012) and Korea (Murugaiyan et al 2010), in which various types of inovirus were detected. It is unclear whether this is because the soil conditions in Thailand (submerged during a rainy season) differ from those in Japan and Korea, or because the Thai bacterial strains have been specifically adapted to Thai conditions.…”

Section: Discussioncontrasting

confidence: 99%

See 1 more Smart Citation

Isolation of Ralstonia solanacearum -infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents

et al. 2015

View full text Add to dashboard Cite

Aims: To isolate and characterize novel bacteriophages infecting the phytopathogen, Ralstonia solanacearum, and to evaluate them as resources with potential uses in the biocontrol of bacterial wilt. Methods and Results: Fourteen phages infecting R. solanacearum were isolated from soil samples collected in Chiang Mai, Thailand. The phages showed different host ranges when tested against 59 R. solanacearum strains isolated from Thailand and Japan. These phages were characterized as nine podoviruses and five myoviruses based on their morphology. Podovirus J2 in combination with another podovirus (φRSB2) lysed host cells very efficiently in contaminated soil. J2 treatment prevented wilting of tomato plants infected with a highly virulent R. solanacearum strain. Conclusions: Treatment with J2 effectively reduced the amount of the bacterial wilt pathogen in contaminated soil and prevented bacterial wilt of tomato in pot experiments. Myovirus J6 possessed jumbo phage features, giving a unique opportunity to study its utilization as a biocontrol agent. Significance and Impact of the Study: As exemplified by J2, the phages isolated in this study represent valuable resources with potential uses in biocontrol of bacterial wilt. A rare jumbo phage J6 served as a valuable subject to understand and utilize this new group of phages.

show abstract

Section: Discussioncontrasting

confidence: 99%

“…) and resembled those of three podoviruses previously isolated in Japan; φ RSB1, φ RSB2 and φ RSB3 (Kawasaki et al . ; Yamada ). The phages were isolated from independent sampling locations, but the same soil sample sometimes yielded different phages, for example, C3 and C6 were obtained from soil sample CM20.…”

Section: Discussionmentioning

confidence: 98%

Isolation of Ralstonia solanacearum -infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Bacteriophages belonging to four viral families ( Podoviridae , Myoviridae , Siphoviridae and Inoviridae ) have been described infecting RSSC strains, and some have shown promise for the control of bacterial wilt disease (Addy et al , ; Elhalag et al , ; Kawasaki et al , , ; Liao, ; Ozawa v, ; Su et al , ; Toyoda et al , ; Van Truong Thi et al , ; Yamada, ) Nevertheless, more studies are needed for a future implementation of phage therapy in the fight against this destructive plant disease (Álvarez and Biosca, ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of CRISPR‐Cas systems in the Ralstonia solanacearum species complex

Xavier

Almeida

Melo

et al. 2018

Molecular Plant Pathology

View full text Add to dashboard Cite

Summary Clustered regularly interspaced short palindromic repeats (CRISPRs) are composed of an array of short DNA repeat sequences separated by unique spacer sequences that are flanked by associated (Cas) genes. CRISPR‐Cas systems are found in the genomes of several microbes and can act as an adaptive immune mechanism against invading foreign nucleic acids, such as phage genomes. Here, we studied the CRISPR‐Cas systems in plant‐pathogenic bacteria of the Ralstonia solanacearum species complex (RSSC). A CRISPR‐Cas system was found in 31% of RSSC genomes present in public databases. Specifically, CRISPR‐Cas types I‐E and II‐C were found, with I‐E being the most common. The presence of the same CRISPR‐Cas types in distinct Ralstonia phylotypes and species suggests the acquisition of the system by a common ancestor before Ralstonia species segregation. In addition, a Cas1 phylogeny (I‐E type) showed a perfect geographical segregation of phylotypes, supporting an ancient acquisition. Ralstoniasolanacearum strains CFBP2957 and K60T were challenged with a virulent phage, and the CRISPR arrays of bacteriophage‐insensitive mutants (BIMs) were analysed. No new spacer acquisition was detected in the analysed BIMs. The functionality of the CRISPR‐Cas interference step was also tested in R. solanacearum CFBP2957 using a spacer‐protospacer adjacent motif (PAM) delivery system, and no resistance was observed against phage phiAP1. Our results show that the CRISPR‐Cas system in R. solanacearum CFBP2957 is not its primary antiviral strategy.

show abstract

“…, ; Addy et al. ,b; Yamada ). However, to our knowledge, there are no studies looking at the interactive effect of phages and antibiotic‐producing bacteria on bacterial wilt dynamics on ecological and evolutionary timescales.…”

mentioning

confidence: 99%

“…This highly complex pathogen is able to adapt to multiple different environmental niches and isolates vary considerably in their host plant range, geographical distribution, pathogenicity, epidemiological relationships, and physiological properties (Genin and Denny 2012). It has been previously shown that both phages and antibiotic-producing bacteria can control R. solanacearum infection to some extent (Wei et al 2011(Wei et al , 2015aAddy et al 2012a,b;Yamada 2012). However, to our knowledge, there are no studies looking at the interactive effect of phages and antibioticproducing bacteria on bacterial wilt dynamics on ecological and evolutionary timescales.…”

mentioning

confidence: 99%

Parasites and competitors suppress bacterial pathogen synergistically due to evolutionary trade-offs

Wang

Wei

et al. 2016

Evolution

View full text Add to dashboard Cite

Parasites and competitors are important for regulating pathogen densities and subsequent disease dynamics. It is, however, unclear to what extent this is driven by ecological and evolutionary processes. Here, we used experimental evolution to study the eco‐evolutionary feedbacks among Ralstonia solanacearum bacterial pathogen, Ralstonia‐specific phage parasite, and Bacillus amyloliquefaciens competitor bacterium in the laboratory and plant rhizosphere. We found that while the phage had a small effect on pathogen densities on its own, it considerably increased the R. solanacearum sensitivity to antibiotics produced by B. amyloliquefaciens. Instead of density effects, this synergy was due to phage‐driven increase in phage resistance that led to trade‐off with the resistance to B. amyloliquefaciens antibiotics. While no evidence was found for pathogen resistance evolution to B. amyloliquefaciens antibiotics, the fitness cost of adaptation (reduced growth) was highest when the pathogen had evolved in the presence of both parasite and competitor. Qualitatively similar patterns were found between laboratory and greenhouse experiments even though the evolution of phage resistance was considerably attenuated in the tomato rhizosphere. These results suggest that evolutionary trade‐offs can impose strong constraints on disease dynamics and that combining phages and antibiotic‐producing bacteria could be an efficient way to control agricultural pathogens.

show abstract

Bacteriophages of Ralstonia solanacearum: Their Diversity and Utilization as Biocontrol Agents in Agriculture

Cited by 21 publications

References 88 publications

Isolation of Ralstonia solanacearum -infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents

Isolation of Ralstonia solanacearum -infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents

Characterization of CRISPR‐Cas systems in the Ralstonia solanacearum species complex

Parasites and competitors suppress bacterial pathogen synergistically due to evolutionary trade-offs

Contact Info

Product

Resources

About