Bacteriophages to control Vibrio alginolyticus in live feeds prior to their administration in larviculture

Abstract

Aims This study aimed to evaluate the efficiency of two phages [VB_VaC_TDDLMA (phage TDD) and VB_VaC_SRILMA (phage SRI)] alone and in a cocktail to control Vibrio alginolyticus in brine shrimp before their administration in larviculture. Methods and results Phages were isolated from seawater samples and characterized by host spectrum, growth parameters, adsorption rate, genomic analysis and inactivation efficiency. Both phages belong to the Caudoviricetes class and lack known virulence or antibiotic-resistance genes. They exhibit specificity, infecting only their host, V. alginolyticus CECT 521. Preliminary experiments in a culture medium showed that phage TDD (reduction of 5.8 log CFUml−1 after 10 h) outperformed phage SRI (reduction of 4.6 log CFUml−1 after 6 h) and the cocktail TDD/SRI (reduction of 5.2 log CFUml−1 after 8 h). In artificial marine water experiments with Artemia franciscana, both single phage suspensions and the phage cocktail, effectively inactivated V. alginolyticus in culture water (reduction of 4.3, 2.1 and 1.9 log CFUml−1 for phages TDD, SRI and the phage cocktail, respectively, after 12 h) and in A. franciscana (reduction of 51.6, 87.3 and 85.3% for phages TDD, SRI and the phage cocktail, respectively, after 24 h). The two phages and the phage cocktail did not affect A. franciscana natural microbiota or other Vibrio species in the brine shrimp. Conclusions The results suggest that phages can safely and effectively control V. alginolyticus in A. franciscana prior to its administration in larviculture.