Bacteriorhodopsin Analogs from Diphenylpolyene Chromophores¶

Singh, Anil Kumar; Manjula, D.

doi:10.1562/0031-8655(2003)0780503bafdc2.0.co2

Cited by 3 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(57) and (58). As an example, synthesis (59) of a retinal analogue namely 3‐methyl‐5‐{4‐[( E )‐2‐phenylvinyl]phenyl}penta‐2 E ,4 E ‐dienal is illustrated in Fig. 4.…”

Section: Studies Of Stereo‐electronic Features Of Br Binding Site Andmentioning

confidence: 99%

“…Some bR‐analogues prepared by different researchers are given in Table 2. We have prepared bR analogues based on iodophenyl (60), anthryl (61), azobenzene (62), diphenylpolyene (59) and indolyl (63) retinal analogues. These are characterized for their absorption behavior, opsin shift, competitive binding, fluorescence emission, light‐induced pH activity and chromophore photochemistry.…”

Section: Studies Of Stereo‐electronic Features Of Br Binding Site Andmentioning

confidence: 99%

See 1 more Smart Citation

Development of Bacteriorhodopsin Analogues and Studies of Charge Separated Excited States in the Photoprocesses of Linear Polyenes†

Singh¹,

Hota²

2007

Photochemistry and Photobiology

Self Cite

View full text Add to dashboard Cite

Development of bacteriorhodopsin (bR) analogues employing chromophore substitution technique for the purpose of characterizing the binding site of bR and generating bR analogues with novel opto-electronic properties for applications as photoactive element in nanotechnical devices are described. Additionally, the photophysical and photochemical properties of variously substituted diarylpolyenes as models of photobiologically relevant linear polyenes are discussed. The role of charge separated dipolar excited states in the photoprocesses of linear polyenes is highlighted.

show abstract

“…(57) and (58). As an example, synthesis (59) of a retinal analogue namely 3‐methyl‐5‐{4‐[( E )‐2‐phenylvinyl]phenyl}penta‐2 E ,4 E ‐dienal is illustrated in Fig. 4.…”

Section: Studies Of Stereo‐electronic Features Of Br Binding Site Andmentioning

confidence: 99%

Section: Studies Of Stereo‐electronic Features Of Br Binding Site Andmentioning

confidence: 99%

Development of Bacteriorhodopsin Analogues and Studies of Charge Separated Excited States in the Photoprocesses of Linear Polyenes†

Singh¹,

Hota²

2007

Photochemistry and Photobiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among the engineering applications of proteins, energy harvesting is significant due to the ubiquity of biological stimulus and the protein required for energy conversion. The most prominent protein-based photoelectric devices (Lanyi, 1993;Luecke et al, 1998;Ho et al, 2004) use the purple protein bacteriorhodopsin and its analogs (Bayley et al, 1981;Druzhko et al, 1996;Hiraki et al, 2002;Singh and Manjula, 2003) to generate transmembrane proton gradient from incident light. In addition to bacteriorhodopsin and its analogs, plant cell membranes have an abundance of proton transporters that are essential for nutrient transport and growth (Buch-Pedersen and Palmgren, 2003;Carpaneto et al, 2005;Sondergaard et al, 2004;Briskin and Reynolds-Niesman, 1991;Briskin, 2005;Nelson and Taiz, 1989).…”

Section: Introductionmentioning

confidence: 99%

Chemoelectrical Energy Conversion of Adenosine Triphosphate using ATPases

Sundaresan

Leo

2009

Journal of Intelligent Material Systems and Structures

View full text Add to dashboard Cite

Biological ion transport processes in proteins have inspired the development of bio-sensors, actuators, photoelectric, and chemoelectric energy conversion devices. These bio-inspired devices use ion transport through a protein energized by biochemical reactions in the protein’s sub-units to perform their engineering function. In an effort to advance the use of biological processes in synthetic systems, a chemoelectrical energy conversion device is demonstrated in this article that uses hydrolysis of adenosine triphosphate (ATP) in ATPase enzyme to generate electrical power. The ATPase enzyme in the device is reconstituted in a bilayer lipid membrane (BLM) and supported on a porous substrate. ATP is dissolved in pH7 buffer and added to one of the chambers in this bicameral device. The transmembrane gradient established by proton transport, resulting from hydrolysis of ATP in the enzyme, is converted into electron flow in an external circuit via silver-silver chloride electrodes placed in the buffer solution on both the sides of the membrane. The chemoelectrical energy conversion of ATP is demonstrated in this article using electrical impedance spectroscopy and load characterization experiments on BLMs supported in the pore(s) of a 25% porous polycarbonate membrane and single-pore silicon nitride chip. In electrical impedance spectroscopy, the change in conductance states of the membrane quantified by specific resistance is used to demonstrate protein activity. The mean electrical impedance of the BLM with ATPase supported on a single pore silicon nitride chip drops from 7 kΩ cm2 to 250 Ω cm2 on adding ATP to one side of the membrane. This change in ionic conductance of the BLM with ATPase in the presence of ATP demonstrates protein activity in the membrane. Impedance analysis of the membranes (BLM, BLM with ATPase) supported in multi-pore polycarbonate substrate demonstrates similar trend confirming ion transport and energy conversion in the membrane. In load characterization experiments, a resistive load of known magnitude is connected to the device and voltage across the membrane and current through the circuit are measured. The current−voltage characteristics of the device resembles a constant current power source and the slope of the response represents the internal resistance of the device. The current through the membrane supported on the single pore substrate is below the range of our data acquisition equipment and hence the device with the porous polycarbonate membrane is used in load characterization experiments. This polycarbonate membrane-based device has an open-circuit voltage of 87.5(±7.5) mV with a specific power output of 1.85 μW/cm2 and an internal resistance of 30.5k(±8.5k)Ω. The theoretical maximum specific power available from the membrane supported on the multi-pore polycarbonate membrane and the single pore silicon-nitride substrate are estimated from the current−voltage response to be 7.65 and 18 μW/cm2.

show abstract

Synthesis of N‐Heteroaryl Retinals and their Artificial Bacteriorhodopsins

et al. 2005

View full text Add to dashboard Cite

N-Heteroaryl retinals derived from indole, 1-indolizine and 3-indolizine (10 a-c) have been synthesized after their UV/Vis red-shifted absorption properties had been predicted by time-dependent density functional theory (TD-DFT) computations. The three new analogues form artificial pigments upon recombination with bacterioopsin: indolyl retinal 10 a undergoes fast and efficient reconstitution to form a species with a UV/Vis absorbance maximum similar to that of wild-type bacteriorhodopsin, whilst the indolizinyl retinals 10 b and 10 c also reconstitute in significant proportion to give noticeably red-shifted, although unstable, pigments. Significant changes in the pK(a) values of these artificial bacteriorhodopsins are interpreted as arising from nonoptimal binding-site occupancy by the chromophore due to steric constraints.

show abstract

Bacteriorhodopsin Analogs from Diphenylpolyene Chromophores¶

Cited by 3 publications

References 43 publications

Development of Bacteriorhodopsin Analogues and Studies of Charge Separated Excited States in the Photoprocesses of Linear Polyenes†

Development of Bacteriorhodopsin Analogues and Studies of Charge Separated Excited States in the Photoprocesses of Linear Polyenes†

Chemoelectrical Energy Conversion of Adenosine Triphosphate using ATPases

Synthesis of N‐Heteroaryl Retinals and their Artificial Bacteriorhodopsins

Contact Info

Product

Resources

About