Bacteriorhodopsin of purple membrane reverses anisotropy outside the pH range of proton pumping based on logic gate realization

Abstract

The bacteriorhodopsin of purple membrane is the first discovered light-sensing protein among ion transporting microbial rhodopsins, some of which (e.g. Archaerhodopsin 3) could be broadly used as tools in optogenetics having wide potential of medical applications. Since its discovery as early as in 1971, bacteriorhodopsin has attracted wide interests in nano-biotechnology, particularly in optoelectronics devices. Therefore, the present work has been motivated due to two topics; firstly, anisotropy demand became indispensible in bioelectronics; secondly, the stationary level of electric response in bacteriorhodopsin within the pH range of proton pumping (pH 3 – pH 10) implies, in turn, raising here a question about whether the electric anisotropy is implicated for reducing (or switching off) such level beyond such pH range. Noteworthy is that the purple membrane converts to blue form upon acidification, while to reddish purple form upon alkalization. In the present study, the acidic and alkaline forms of bacteriorhodopsin have exhibited most probable state of reversal for the dielectric anisotropy around pH 2.5 and pH 10.5, respectively. This is underscored by proposing a correlation seemingly found between disassembly of the crystalline structure of bacteriorhodopsin and the reversal of dielectric anisotropy, at such acidic and alkaline reversal pH’s, in terms of the essence of the crystalline lattice. Most importantly, the results have substantiated dual frequency characteristics and logic gate-based dielectric anisotropy reversal to bacteriorhodopsin, which may implicate it for potential applications in bioelectronics.