Bacteriostatic effect of orally administered bovine lactoferrin on proliferation of Clostridium species in the gut of mice fed bovine milk

Teraguchi, Susumu; Shin, Kouichirou; Ozawa, Kazuhiro; Nakamura, S.-I.; Fukuwatari, Yasuo; Tsuyuki, Shigeo; Namihira, H; Shimamura, Seiichi

doi:10.1128/aem.61.2.501-506.1995

Cited by 62 publications

(19 citation statements)

References 14 publications

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“…A decreased number of Clostridium spp. were detected in the faeces of mice fed milk supplemented with either bovine lactoferrin (bLF) or pepsin‐derived bLF hydrolysate, in an experimental model of colonization of Clostridium [26]. Thus, antimicrobial activities of hLF or peptides could have contributed to the reduced symptoms in the hLF‐ and peptide‐treated, DX‐exposed animals in our study.…”

Section: Discussionmentioning

confidence: 73%

Anti‐Inflammatory Activities of Human Lactoferrin in Acute Dextran Sulphate‐Induced Colitis in Mice

et al. 2003

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In this study, we investigated the anti-inflammatory effects of orally administered human lactoferrin (hLF) and two peptides, based on the bactericidal region of hLF (HLD1 and HLD2), on the course of experimental colitis. Acute colitis was induced in C57Bl/6 mice by giving 5% dextran sulphate (DX) in the drinking water. The mice were killed after 2 or 7 days of DX exposure. The animals were given hLF or the peptides orally twice a day (2 mg/dose/mouse) during the DX exposure. In the control animals, the hLF or the peptides were replaced by bovine serum albumin or water.The appearance of occult blood in the faeces and macroscopic rectal bleeding were significantly delayed and partly reduced in the hLF-treated animals compared with the control animals. The shortening of the colon, a pathological effect of DX exposure, was significantly less pronounced in the hLF-treated group compared with the control group. Also, the interleukin-1b (IL-1b) levels in the blood were significantly diminished in this group after 2 days of DX exposure. A significantly lower crypt score was observed in the distal part of the colon in the hLF-treated group compared with the control group. Also, significantly reduced numbers of CD4 cells, F4/80-positive macrophages and tumour necrosis factor-a-producing cells were detected by immunohistochemistry in the distal colon of the hLF-treated animals compared with the control animals after 7 days of DX exposure. A reduction was also observed concerning the IL-10-producing cells in the middle colonic submucosa. The HLD1 and HLD2 treatment, which was carried out for 2 days, only gave results almost identical to those of hLF, concerning clinical parameters after the 2 days of DX exposure. An even stronger effect was observed for HLD2, regarding decreased occult blood in the faeces and colon length.Our results show that perorally given hLF mediates anti-inflammatory effects on the DX-induced acute colitis, and further suggest that the bactericidal region of the hLF molecule may be involved in these activities.

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Section: Discussionmentioning

confidence: 73%

Anti‐Inflammatory Activities of Human Lactoferrin in Acute Dextran Sulphate‐Induced Colitis in Mice

et al. 2003

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“…Recent studies have pointed towards the bacteriostatic effects of ingested Lf against Enterobacteriaceae and intestinal Clostridia and the inhibition of bacterial translocation from the intestines to the mesentric lymph nodes (Teraguchi et al . 1994, 1995a,b), but no systematic studies of these effects have been published yet.…”

mentioning

confidence: 99%

Influence of lactoferrin feeding and injection against systemic staphylococcal infections in mice

1999

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R .S . B H IM AN I , Y . V E ND RO V AN D P . FU RM A NS KI . 1999. Human and bovine lactoferrins (Lfs) and bovine lactoferrin hydrolysate (LH) were assessed in vitro and in vivo for their antibacterial effects on Staphylococcus aureus. Lactoferrins showed weak in vitro antibacterial activity while Fe-saturated Lfs and LH showed no activity. Lactoferrin-treated mice (1 mg, i.v.) when injected i.v. with 10 6 staphylococci, showed 30-50% reduction in kidney infections, and viable bacterial counts in the kidneys decreased 5-12-fold. The inhibitory effect was dosedependent up to 1 mg Lf. Lactoferrins were effective when given 1 day prior to the bacterial challenge, after which there was no significant effect even at doses up to 5 mg. Apo-and Fesaturated forms of human and bovine Lfs were all equally effective, while LH was not protective. Human and bovine Lfs with different degrees of iron saturation (9-97%) were found to be equipotent. Feeding mice with 2% bLf in drinking water also reduced the kidney infections by 40-60%, and viable bacterial counts, 5-12-fold. The results suggest a potential for the use of Lfs as natural antibacterial proteins for preventing bacterial infections.

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“…The occurrence of lactoferrin in biological fluids like milks, tears, saliva and seminal fluids suggests that it could have a role in the nonspecific defence against invading pathogens. Its broad antimicrobial spectrum (including Gram‐positive and Gram‐negative bacteria, yeasts and fungi) and the recently discovered antiviral activity (including cytomegalovirus, herpes, influenza, HIV, rotavirus, hepatitis C) support this envisaged role 53–61 …”

Section: Minor Bioactive Proteins From Milk: Immunoglobulins and Lactmentioning

confidence: 94%

Milk ingredients as nutraceuticals

Steijns¹

2001

Int J of Dairy Tech

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Cow’s milk is considered as a basic food in many diets, and is rich in a variety of essential nutrients. With today’s sophisticated analytical, biochemical and cell‐biological research tools, the presence of many other (minor) compounds with biological activity has been demonstrated. Achievements in separation techniques in the dairy industry and enzyme technology offer opportunities to isolate, concentrate or modify these compounds, so that their application in functional foods, dietary supplements, nutraceuticals and medical foods has become possible. Within the sequence of amino acids of a dietary protein, specific peptides may be located with specific biological activity. Peptides from casein may be used to enhance the solubility of minerals such as calcium and zinc—and hence increase the bioavailability of these minerals—or to reduce the activity of the angiotensin‐converting enzyme, which is involved in vasoconstriction and (hence) blood pressure. Immunoglobulins from vaccinated cows may be considered as natural antimicrobials with certain advantages over synthetic antibiotics. Lactoferrin is an example of a minor milk protein that has been studied in great detail: it is becoming increasingly clear that it is important for the nonspecific defence against bacteria, fungi and viruses. Oligosaccharides, glycolipids and glycoproteins containing sialic acid residues may have a role as anti‐infectives. Components such as growth factors may also be considered for future product development, because of the economies of scale used in the dairy industry.

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Bacteriostatic effect of orally administered bovine lactoferrin on proliferation of Clostridium species in the gut of mice fed bovine milk

Cited by 62 publications

References 14 publications

Anti‐Inflammatory Activities of Human Lactoferrin in Acute Dextran Sulphate‐Induced Colitis in Mice

Anti‐Inflammatory Activities of Human Lactoferrin in Acute Dextran Sulphate‐Induced Colitis in Mice

Influence of lactoferrin feeding and injection against systemic staphylococcal infections in mice

Milk ingredients as nutraceuticals

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