Bacterization of potato micro-plants in vitro by Streptomyces rhizobacteria increases the efficiency of cultivation

Abstract

In order to grow high-quality seed potatoes more efficiently, it is necessary constantly to improve cultivation technologies and accelerated reproduction of in vitro material under aseptic conditions. During the research it has been established that inoculation with a local strain of Streptomyces minoensis KR-10, which was isolated from the rhizosphere of Urtica dioica L. with the help of selective reception, allows to optimize the conditions of in vitro cultivation of the material at the cloning stages. The strain is characterized by phytostimulating activity, the ability to synthesize auxins (18.7±1.0 µg/ml), high radial growth rate (65.7±8.8 µm/h) and colonizing ability (105–108 CFU/g depending on the type of plant tissue). The optimal inoculation method was identified, which provides for sequential dipping of micro gears into a bacterial suspension, and the titer of the bacterial suspension (105 CFU/ml) was determined, contributing to an increase in the reproduction coefficient of the promising breeding number 172-13 and the ‘Pransa’ cultivar. The severity of bacterization effects depended on the genotypic characteristics of potatoes. Inoculation with S. minoensis strain KR-10 made it possible to reduce the proportion of morphoses among in vitro material number 172-13 by 2.5 times. The developed techniques are able to increase the efficiency of growing potato biomaterial in the process of accelerated clonal reproduction.