Bacteroides fragilis enterotoxin modulates epithelial permeability and bacterial internalization by HT-29 enterocytes

Wells, Carol L.; Westerlo, Elisabeth M.A. van de; Jechorek, Robert P.; Feltis, Brad A.; Wilkins, Tracy D.; Erlandsen, Stanley L.

doi:10.1053/gast.1996.v110.pm8613048

Cited by 94 publications

(85 citation statements)

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“…In a series of experiments, Onderdonk et al (17) demonstrated that B. vulgatus was the dominant bacterial stimulus in carrageenan-induced colitis in guinea pigs. Furthermore, Bacteroides fragilis enterotoxin increases mucosal permeability and epithelial internalization of enteric bacteria (43). Together, these results demonstrate a consistent role for Bacteroides spp., especially B. vulgatus, in the pathogenesis of chronic intestinal inflammation.…”

Section: Discussionsupporting

confidence: 60%

Normal luminal bacteria, especially Bacteroides species, mediate chronic colitis, gastritis, and arthritis in HLA-B27/human beta2 microglobulin transgenic rats.

et al. 1996

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Genetic and environmental factors are important in the pathogenesis of clinical and experimental chronic intestinal inflammation. We investigated the influence of normal luminal bacteria and several groups of selected bacterial strains on spontaneous gastrointestinal and systemic inflammation in HLA-B27 transgenic rats. Rats maintained germfree for 3-9 mo were compared with littermates conventionalized with specific pathogen-free bacteria. Subsequently, germfree transgenic rats were colonized with groups of five to eight bacteria that were either facultative or strictly anaerobic. Transgenic germfree rats had no gastroduodenitis, colitis, or arthritis, but developed epididymitis and dermatitis to the same degree as conventionalized rats. Colonic proinflammatory cytokine expression was increased in transgenic conventionalized rats but was undetectable in germfree and nontransgenic rats.

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Section: Discussionsupporting

confidence: 60%

Normal luminal bacteria, especially Bacteroides species, mediate chronic colitis, gastritis, and arthritis in HLA-B27/human beta2 microglobulin transgenic rats.

et al. 1996

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“…All these factors may lead to sepsis and multiorgan failure [42][43][44][45][46] . The treatment of SIBO must firstly focus on the correction of wrong food and dietary habits that usually underlie the disorder (e.g.…”

Section: Discussionmentioning

confidence: 99%

Breath test for differential diagnosis between small intestinal bacterial overgrowth and irritable bowel disease: An observation on non-absorbable antibiotics

Esposito¹,

Leone²,

Gregorio³

et al. 2007

WJG

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“…Actin filaments are a key in maintaining cell shape and regulating tight junction permeability, and several bacterial enterotoxins previously have been found to alter actin microfilaments (27,42,47). We therefore examined if the viral NSP4 toxin had any effect on the distribution of F-actin in MDCK-1 cells.…”

Section: Monolayers (Day 3)mentioning

confidence: 99%

“…Disruption or interference of intestinal epithelial tight junctions may therefore contribute to microbe-associated diarrhea. Since the distributions of both filamentous actin (F-actin) and Zona Occludens-1 (ZO-1) are altered by certain bacteria and their toxins (17,25,36,48), it is considered that these structural changes are directly or indirectly involved in the pathogenesis of intestinal diseases (17,47,48).…”

mentioning

confidence: 99%

NSP4 Enterotoxin of Rotavirus Induces Paracellular Leakage in Polarized Epithelial Cells

Tafazoli

Zeng

Estes

et al. 2001

J Virol

112

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The nonstructural NSP4 protein of rotavirus has been described as the first viral enterotoxin. In this study we have examined the effect of NSP4 on polarized epithelial cells (MDCK-1) grown on permeable filters. Apical but not basolateral administration of NSP4 was found to cause a reduction in the transepithelial electrical resistance, redistribution of filamentous actin, and an increase in paracellular passage of fluorescein isothiocyanate-dextran. Significant effects on transepithelial electrical resistance were noted after a 20-to 30-h incubation with 1 nmol of NSP4. Most surprisingly, the epithelium recovered its original integrity and electrical resistance upon removal of NSP4. Preincubation of nonconfluent MDCK-1 cells with NSP4 prevented not only development of a permeability barrier but also lateral targeting of the tight-junction-associated Zonula Occludens-1 (ZO-1) protein. Taken together, these data indicate new and specific effects of NSP4 on tight-junction biogenesis and show a novel effect of NSP4 on polarized epithelia.

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Bacteroides fragilis enterotoxin modulates epithelial permeability and bacterial internalization by HT-29 enterocytes

Cited by 94 publications

References 0 publications

Normal luminal bacteria, especially Bacteroides species, mediate chronic colitis, gastritis, and arthritis in HLA-B27/human beta2 microglobulin transgenic rats.

Normal luminal bacteria, especially Bacteroides species, mediate chronic colitis, gastritis, and arthritis in HLA-B27/human beta2 microglobulin transgenic rats.

Breath test for differential diagnosis between small intestinal bacterial overgrowth and irritable bowel disease: An observation on non-absorbable antibiotics

NSP4 Enterotoxin of Rotavirus Induces Paracellular Leakage in Polarized Epithelial Cells

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