Bacteroides graminisolvens sp. nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste

Nishiyama, Tomomi; Ueki, Atsuko; Kaku, Nobuo; Watanabe, Kazuya; Ueki, Katsuji

doi:10.1099/ijs.0.008268-0

Cited by 105 publications

(57 citation statements)

References 34 publications

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“…Possibly, the disruption of floc structure or other effects on substrate characteristics by the MW pre-treatment could have induced this change in Bacteroidetes community structure. On species level, Bacteroides cellulosolvens and Bacteroides graminisolvens that have been shown to degrade cellulose and xylan, respectively (Murray 1986;Nishiyama et al 2009), were detected in all three digesters.…”

Section: Bacterial Taxonomic Distributionmentioning

confidence: 97%

Microwave and ultrasound pre-treatments influence microbial community structure and digester performance in anaerobic digestion of waste activated sludge

Westerholm

Crauwels

Geel

et al. 2016

Appl Microbiol Biotechnol

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Comparative analyses of bacterial and archaeal community structures and dynamics in three biogas digesters during start-up and subsequent operation using microwaved, ultrasonicated or untreated waste activated sludge were performed based on 454 pyrosequencing datasets of part of 16S ribosomal RNA sequences and quantitative PCR. The pre-treatment increased the solubility, and thus the availability of the substrate for microbial degradation and significantly affected the succession of the anaerobic community structure over the course of the digestion. Bacteroidetes, Proteobacteria and Firmicutes were the dominant phyla in all digesters throughout operation. Proteobacteria decreased in relative abundance from 23-26 % to 11-13 % in association with enhanced substrate availability. Negative correlations between relative abundance of Alpha-, Beta- and Gammaproteobacteria and the substrate availability and/or biogas production were disclosed in statistical analyses. Clostridiales was the dominant order in Firmicutes, and Clostridiales, Clostridia and Firmicutes relative abundance and richness were shown to positively correlate with substrate availability and biogas generation. Methanogenic communities had a fairly restricted structure, highly dominated by Methanosaeta and Methanobrevibacter phylotypes. A gradual decline in Methanobrevibacter and increased representation of Methanosaeta concilii over time were particularly apparent in the digester receiving untreated waste activated sludge, whereas more diversified archaeal communities were maintained in the pre-treatment digesters. The quantitative PCR analyses revealed a methanogenic community distribution that coincided with the 454 pyrosequencing data.

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Section: Bacterial Taxonomic Distributionmentioning

confidence: 97%

Microwave and ultrasound pre-treatments influence microbial community structure and digester performance in anaerobic digestion of waste activated sludge

Westerholm

Crauwels

Geel

et al. 2016

Appl Microbiol Biotechnol

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“…58 ABE et al mal specimens, and they are the most dominant members in the bacterial community of human feces (Holdeman et al, 1984;Paster et al, 1994). Only a few species within the four families of anaerobes have been isolated and described from habitats other than human or animal specimens (Nishiyama et al, 2009;Ueki et al, 2006Ueki et al, , 2008Ueki et al, , 2011Whitehead et al, 2005). Out of the four families, Rikenellaceae currently includes two genera (Rikenella and Alistipes) (Ludwig et al, 2008).…”

mentioning

confidence: 99%

Anaerocella delicata gen. nov., sp. nov., a strictly anaerobic bacterium in the phylum Bacteroidetes isolated from a methanogenic reactor of cattle farms

Abe

Ueki

Ohtaki

et al. 2012

J. Gen. Appl. Microbiol.

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IntroductionThe phylum Bacteroidetes is currently divided into four classes (Ludwig et al., 2008), and contains both strictly anaerobic (mainly in the order Bacteroidales in the class Bacteroidia ) and aerobic (in the classes Flavobacteria, Sphingobacteria and Cytophagia ) members. Most of strictly anaerobic species in the four families (Bacteroidaceae, Rikenellaceae , Porphyromonadaceae and Prevotellaceae ) other than Marinilabiliaceae (Suzuki et al., 1999) in the order Bacteroidales have been isolated from human or ani-J. Gen. Appl. Microbiol., 58, 405 412 (2012) A strictly anaerobic bacterial strain (WN081 T ) was isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms in Japan. Cells were Gram-staining negative, nonmotile, non-spore-forming straight rods. The strain grew rather well on PY agar slants supplemented with a B-vitamin mixture as well as sugars (PYV4S medium) and made translucent and glossy colonies. Growth in liquid medium with the same composition, however, was scanty, and growth was not improved in spite of various additives to the medium. Strain WN081 T produced small amounts of acetate, propionate, isobutyrate, butyrate, isovalerate and H 2 from PYV liquid medium. The strain did not use carbohydrates or organic acids. The pH range for growth was narrow (pH 6.8 8.2), having a pH optimum at 6.8 7.5. The temperature range for growth was 10 37°C, the optimum being 25 30°C. The strain was sensitive to bile, and did not have catalase or oxidase activities. Hydrogen sulfi de was produced from L-cysteine and L-methionine as well as peptone. Indole was produced from L-tryptophan and peptone. The strain had iso-C 15:0 as the exclusively predominant cellular fatty acid (70%) together with some branched chain components (such as iso-C 15:0 DMA, iso-C 17:0 3-OH and iso-C 15 : 0 aldehyde) as minor components. The genomic DNA G+C content was 32.3 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain WN081 T in the phylum Bacteroidetes with rather low sequence similarities with the related species such as Rikenella microfusus (85.7% sequence similarity), Alistipes putredinis (85.5%) and Alistipes fi negoldii (85.5%) in the family Rikenellaceae. Based on the phylogenetic, physiological and chemotaxonomic analyses, the novel genus and species Anaerocella delicata gen. nov., sp. nov. is proposed to accommodate the strain. The type strain is WN081 T (= JCM 17049 T = DSM 23595 T ).

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“…After the adaptation, the major groups were Spirochaetes, Porphyromonadaceae, Bacteroidaceae and Bacilli. Looking at the taxa most closely related to the OTUs found indicated that the community was dominated by microorganisms that anaerobically hydrolyze complex and simple sugars to acids (Table 5 and Table S6) (Dröge et al, 2005;Nishiyama et al, 2009;Song et al, 2005). It is also interesting to note that Synergistetes was not detected in the rumen, was present in only low numbers in the mixed inoculum, but increased to approximately 1% of the population in the final enrichments.…”

Section: Analysis Of the Domain Bacteriamentioning

confidence: 91%

Transition of microbial communities during the adaption to anaerobic digestion of carrot waste

Garcia

Jangid

Whitman

et al. 2011

Bioresource Technology

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Bacteroides graminisolvens sp. nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste

Cited by 105 publications

References 34 publications

Microwave and ultrasound pre-treatments influence microbial community structure and digester performance in anaerobic digestion of waste activated sludge

Microwave and ultrasound pre-treatments influence microbial community structure and digester performance in anaerobic digestion of waste activated sludge

Anaerocella delicata gen. nov., sp. nov., a strictly anaerobic bacterium in the phylum Bacteroidetes isolated from a methanogenic reactor of cattle farms

Transition of microbial communities during the adaption to anaerobic digestion of carrot waste

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