Bacteroides muris sp. nov. isolated from the cecum of wild-derived house mice

Abstract

Two bacterial strains, KH365_2T and KH569_7, were isolated from the cecum contents of wild-derived house mice. The strains were characterized as Gram-negative, rod-shaped, strictly anaerobic, and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains were most closely related to Bacteroides uniformis ATCC 8492T. Whole genome sequences of KH365_2T and KH569_7 strains have a DNA G + C content of 46.02% and 46.03% mol, respectively. Most morphological and biochemical characteristics did not differ between the newly isolated strains and classified Bacteroides strains. However, the average nucleotide identity (ANI) and dDNA–DNA hybridization (dDDH) values clearly distinguished the two strains from described members of the genus Bacteroides. Here, we present the phylogeny, morphology, and physiology of a novel species of the genus Bacteroides and propose the name Bacteroides muris sp. nov., with KH365_2T (DSM 114231T = CCUG 76277T) as type strain.

Closely relatedBacteroidesof the murine intestinal microbiota affect each other’s growth positively or negatively

“…on the growth of another. A total of 169 strain combinations were tested, including 13 strains each belonging to one of three species, B. acidifaciens (n=5), B. caecimuris (n=6), or B. muris (n=2), the latter of which was recently described (25, 26). We grew the strains as monocultures in spent media of self or nonself and measured the optical density (OD) at the final time point as a read-out of bacterial growth.…”

Bacteroides rhinocerotis sp. nov., isolated from the fresh feces of rhinoceros in Beijing Zoo

“…In addition to human faecal samples (Kim et al 2022;Sun et al 2022), a large number of novel Bacteroides species isolated from animals have been described recently. These include those found in the cecum of wild derived house mice (Fokt et al 2022;Clavel et al 2010), the gut of a subterranean termite (Reticulitermes speratus) (Sakamoto and Ohkuma 2013), caecum of chicken (Irisawa et al 2016;Saputra et al 2015), a methanogenic reactor treating waste from cattle farms (Nishiyama et al 2009; Ueki et al 2008Ueki et al , 2011) and chinchilla feces (Kitahara et al 2011). Bacteroides spp.…”

Validation List no. 209. Valid publication of new names and new combinations effectively published outside the IJSEM

“…huaxiensis, pertaining to Huaxi. 11 The effective publication states that the type strain was also deposited as ATCC 53080, but no documentation was supplied. 12 The CGMCC number is found in the abstract, not in the protologue.…”