Bacteroides ovatus colonization influences the abundance of intestinal short chain fatty acids and neurotransmitters

Horvath, Thomas D.; Ihekweazu, Faith D.; Haidacher, Sigmund J.; Ruan, Wenly; Engevik, Kristen A.; Fultz, Robert; Hoch, Kathleen M.; Luna, Ruth Ann; Oezguen, Numan; Spinler, Jennifer K.; Haag, Anthony M.; Versalovic, James; Engevik, Melinda A.

doi:10.1016/j.isci.2022.104158

Cited by 68 publications

(47 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, recent studies found that colonization by B. fragilis, B. uniformis, and B. caccae, but not B. ovatus, recapitulated the negative effects of "depression microbiota" on behavior and neurogenesis, suggesting differential behavioral impacts of bacteria from the same genus. B. ovatus, a human gut commensal bacterium with anti-inflammatory properties and potential as a next-generation probiotic (57, 58), can reportedly influence the abundance of intestinal short-chain fatty acids (SCFAs) and neurotransmitters such as g-aminobutyric acid (GABA) (59). Bacteroides spp.…”

Section: Discussionmentioning

confidence: 99%

Changes in fecal microbiota composition and the cytokine expression profile in school-aged children with depression: A case-control study

et al. 2022

View full text Add to dashboard Cite

Depression in childhood negatively affects the growth and development, school performance, and peer or family relationships of affected children, and may even lead to suicide. Despite this, its etiology and pathophysiology remain largely unknown. Increasing evidence supports that gut microbiota plays a vital role in the development of childhood depression. However, little is known about the underlying mechanisms, as most clinical studies investigating the link between gut microbiota and depression have been undertaken in adult cohorts. In present study, a total of 140 school-aged children (6–12 years) were enrolled, including 92 with depression (male/female: 42/50) and 48 healthy controls (male/female: 22/26) from Lishui, Zhejiang, China. Illumina sequencing of the V3–V4 region of the 16S rRNA gene was used to investigate gut microbiota profiles while Bio-Plex Pro Human Cytokine 27-plex Panel was employed to explore host immune response. We found that, compared with healthy controls, children with depression had greater bacterial richness and altered β-diversity. Pro-inflammatory genera such as Streptococcus were enriched in the depression group, whereas anti-inflammatory genera such as Faecalibacterium were reduced, as determined by linear discriminant analysis effect size. These changes corresponded to altered bacterial functions, especially the production of immunomodulatory metabolites. We also identified the presence of a complex inflammatory condition in children with depression, characterized by increased levels of pro-inflammatory cytokines such as IL-17 and decreased levels of anti-inflammatory cytokines such as IFN-γ. Correlation analysis demonstrated that the differential cytokine abundance was closely linked to changes in gut microbiota of children with depression. In summary, key functional genera, such as Streptococcus and Faecalibacterium, alone or in combination, could serve as novel and powerful non-invasive biomarkers to distinguish between children with depression from healthy ones. This study was the first to demonstrate that, in Chinese children with depression, gut microbiota homeostasis is disrupted, concomitant with the activation of a complex pro-inflammatory response. These findings suggest that gut microbiota might play an important role in the pathogenesis of depression in school-aged children, while key functional bacteria in gut may serve as novel targets for non-invasive diagnosis and patient-tailored early precise intervention in children with depression.

show abstract

Section: Discussionmentioning

confidence: 99%

Changes in fecal microbiota composition and the cytokine expression profile in school-aged children with depression: A case-control study

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Univariate analysis (Mann–Whitney) on plasma metabolites for UV resistant subjects ( n = 9) at Day 15 (D15; following a restricted diet) vs. Day 30 (D30; following two weeks of grape consumption. Reference cited in Supplementary File S5 [ 22 , 23 , 27 , 28 , 38 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 ].…”

mentioning

confidence: 99%

Short-Term Grape Consumption Diminishes UV-Induced Skin Erythema

et al. 2022

View full text Add to dashboard Cite

Over three million Americans are affected by skin cancer each year, largely as a result of exposure to sunlight. The purpose of this study was to determine the potential of grape consumption to modulate UV-induced skin erythema. With 29 human volunteers, we report that nine demonstrated greater resistance to UV irradiation of the skin after consuming the equivalent of three servings of grapes per day for two weeks. We further explored any potential relationship to the gut–skin axis. Alpha- and beta-diversity of the gut microbiome were not altered, but grape consumption modulated microbiota abundance, enzyme levels, and KEGG pathways. Striking differences in the microbiome and metabolome were discerned when comparing the nine individuals showing greater UV resistance with the 20 non-responders. Notably, three urinary metabolites, 2′-deoxyribonic acid, 3-hydroxyphenyl acetic and scyllo-inositol, were depressed in the UV-resistant group. A ROC curve revealed a 71.8% probability that measurement of urinary 2′-deoxyribonic acid identifies a UV skin non-responder. 2′-Deoxyribonic acid is cleaved from the DNA backbone by reactive oxygen species. Three of the nine subjects acquiring UV resistance following grape consumption showed a durable response, and these three demonstrated unique microbiomic and metabolomic profiles. Variable UV skin sensitivity was likely due to glutathione S-transferase polymorphisms. We conclude that a segment of the population is capable of demonstrating greater resistance to a dermal response elicited by UV irradiation as a result of grape consumption. It is uncertain if modulation of the gut-skin axis leads to enhanced UV resistance, but there is correlation. More broadly, it is reasonable to expect that these mechanisms relate to other health outcomes anticipated to result from grape consumption.

show abstract

“…The advantage of using a chemically defined medium is that the supernatant is amenable to downstream LC-MS/MS analysis since it does not contain components such as peptone and yeast extract. We have used this defined medium to examine the growth of diverse microbes and measure bacterial metabolites and proteins by LC-MS/MS [ 19 , 20 , 21 , 22 , 23 ]. However, this medium has not been validated for antibiotic studies, and the current gold standard medium is Mueller Hinton broth.…”

Section: Discussionmentioning

confidence: 99%

Profiling Antibiotic Resistance in Acinetobacter calcoaceticus

2022

Self Cite

View full text Add to dashboard Cite

Background: Acinetobacter spp. have emerged as troublesome pathogens due to their multi-drug resistance. The majority of the work to date has focused on the antibiotic resistance profile of Acinetobacter baumannii. Although A. calcoaceticus strains are isolated in the hospital setting, limited information is available on these closely related species. Methods & Results: The computational analysis of antibiotic resistance genes in 1441 Acinetobacter genomes revealed that A. calcoaceticus harbored a similar repertoire of multi-drug efflux pump and beta-lactam resistance genes as A. baumannii, leading us to speculate that A. calcoaceticus would have a similar antibiotic resistance profile to A. baumannii. To profile the resistance patterns of A. calcoaceticus, strains were examined by Kirby–Bauer disk diffusion and phenotypic microarrays. We found that Acinetobacter strains were moderately to highly resistant to certain antibiotics within fluoroquinolones, aminoglycosides, tetracyclines, and other antibiotic classes. These data indicate that A. calcoaceticus has a similar antibiotic resistance profile as A. baumannii ATCC 19606. We also identified that all Acinetobacter species were sensitive to 5-fluoroorotic acid, novobiocin, and benzethonium chloride. Conclusion: Collectively, these data provide new insights into the antibiotic resistance in A. calcoaceticus and identify several antibiotics that could be beneficial in treating Acinetobacter infections.

show abstract

Bacteroides ovatus colonization influences the abundance of intestinal short chain fatty acids and neurotransmitters

Cited by 68 publications

References 84 publications

Changes in fecal microbiota composition and the cytokine expression profile in school-aged children with depression: A case-control study

Changes in fecal microbiota composition and the cytokine expression profile in school-aged children with depression: A case-control study

Short-Term Grape Consumption Diminishes UV-Induced Skin Erythema

Profiling Antibiotic Resistance in Acinetobacter calcoaceticus

Contact Info

Product

Resources

About