Bacteroides ovatus GH5 xyloglucanase in complex with a XXXG heptasaccharide

Larsbrink, Johan; Rogers, Theresa; Hemsworth, G.R.; McKee, Lauren S.; Spadiut, Oliver; Klinter, Stefan; Pudlo, Nicholas A.; Urs, Karthik; Kelly, Andrew G.; Cederholm, Stefan Nilsson; Davies, G.J.; Martens, Eric C.; Brumer, Harry

doi:10.2210/pdb3zmr/pdb

2014

DOI: 10.2210/pdb3zmr/pdb

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Bacteroides ovatus GH5 xyloglucanase in complex with a XXXG heptasaccharide

Johan Larsbrink¹,

Theresa Rogers²,

G.R. Hemsworth³

et al.

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A Genetically Adaptable Strategy for Ribose Scavenging in a Human Gut Symbiont Plays a Diet-Dependent Role in Colon Colonization

Glowacki

Pudlo

Tunçil³

et al. 2019

SSRN Journal

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Efficient nutrient acquisition in the competitive human gut is essential for microbial persistence. While polysaccharides have been well-studied nutrients for the gut microbiome, other resources such as co-factors and nucleic acids have been less examined. We describe a series of ribose utilization systems (RUSs) that are broadly represented in Bacteroidetes and appear to have diversified to allow access to ribose from a variety of substrates. One Bacteroides thetaiotaomicron RUS variant is critical for competitive gut colonization in a diet-specific fashion. Using molecular genetics, we probed the nature of the ribose source underlying this dietspecific phenotype, revealing that hydrolytic functions in RUS (e.g., to cleave ribonucleosides) are present but dispensable. Instead, ribokinases that are activated in vivo and participate in cellular ribose-phosphate metabolism are essential. Our results underscore the extensive mechanisms that gut symbionts have evolved to access nutrients and how metabolic context determines the impact of these functions in vivo.

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A Genetically Adaptable Strategy for Ribose Scavenging in a Human Gut Symbiont Plays a Diet-Dependent Role in Colon Colonization

Glowacki

Pudlo

Tunçil³

et al. 2019

SSRN Journal

View full text Add to dashboard Cite

show abstract

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Bacteroides ovatus GH5 xyloglucanase in complex with a XXXG heptasaccharide

Cited by 1 publication

References 0 publications

A Genetically Adaptable Strategy for Ribose Scavenging in a Human Gut Symbiont Plays a Diet-Dependent Role in Colon Colonization

A Genetically Adaptable Strategy for Ribose Scavenging in a Human Gut Symbiont Plays a Diet-Dependent Role in Colon Colonization

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