Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

AcMNPV-BmK IT improves the progeny virus production via baculovirus GP64 envelope fusion protein

“…AcMNPV-BmK ITs (IE1, P10, PH) were constructed in our laboratory (Fan et al 2008). Sf9 cells were cultured in our laboratory.…”

“…In our previous work, we engineered BmK IT gene into the genome of AcMNPV under the regulation of IE1, P10 protein (P10) and polyhedrosis (PH) promoter, respectively. The bioassay data indicated that the recombinant baculovirus AcMNPV-BmK IT significantly enhanced the anti-insect efficacy of the virus (Fan et al 2008).…”

Regulation analysis of AcMNPV-mediated expression of a Chinese scorpion neurotoxin under the IE1, P10 and PH promoter in vivo and its use as a potential bio-insecticide

“…Three recombinant AcMNPV-BmK IT (IE1), AcMNPV-BmK IT(P10) and AcMNPV-BmK IT(PH) were previously constructed in our laboratory (Fan 2008;Fan et al 2008). The larvae of Heliothis armigera were cultured in laboratory conditions (30°C, 70 % of relative humidity) and individually reared on an artificial diet.…”

Expression of Ac-PK2 protein from AcMNPV improved the progeny virus productionviaregulation of energy metabolism and protein synthesis

“…In our previous work, we constructed recombinant virus AcMNPV-BmK IT, and found that it obviously improved the anti-insect activity of baculovirus. 9 In the present work, AcMNPV-PK2-EGFP was constructed by Bac-to-Bac system to study the tness and insecticidal activity of AcMNPV aer the overexpression of Ac-PK2 protein. Our results showed that Ac-PK2 protein could inhibit eIF2a kinase phosphorylation in Sf9 cell, compared with AcMNPV treatment group.…”