Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells

Duisit, Ghislaine; Saleün, Sylvie; Douthe, S.; Barsoum, James; Chadeuf, Gilliane; Moullier, P.

doi:10.1002/(sici)1521-2254(199903/04)1:2<93::aid-jgm19>3.3.co;2-t

Cited by 36 publications

(58 citation statements)

References 27 publications

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“…Continued analysis has shown that baculovirus can infect and mediate gene transfer to cell types other than hepatic cells (14,36,42,45). However, this system has not proven applicable to all cell types.…”

Section: Discussionmentioning

confidence: 95%

“…Although the binding motif responsible for baculovirus endocytosis in mammalian cells is currently unknown, heparan sulfate is required for efficient gene transfer (14). The data presented in this study demonstrate that at least in some cell types, such as hepatocytes, entry of the baculovirus may require contact with the basolateral surface.…”

Section: Discussionmentioning

confidence: 98%

“…No significant cytopathology was observed at the EGTA concentrations or MOIs affording the maximal gene transfer efficiency. A previous study reported that cytopathology was not observed when HepG2 cells were infected at a multiplicity of 5,000 PFU of baculovirus/cell (14). Therefore, it is possible that the percentage of primary rat hepatocytes positive for baculovirus-mediated gene transfer may be increased to Ͼ75% if the MOI is increased to more than 1,600 PFU/cell.…”

Section: Discussionmentioning

confidence: 99%

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Transient Disruption of Intercellular Junctions Enables Baculovirus Entry into Nondividing Hepatocytes

Bilello¹,

Delaney²,

Boyce

et al. 2001

J Virol

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Baculovirus infection has extended the capabilities for transfection of exogenous genes into a variety of mammalian cell types. Because rat hepatocytes plated on collagen-coated dishes and maintained in dimethyl sulfoxide (DMSO)-supplemented chemically defined medium are an excellent model system for studying liver function in vitro, we investigated the ability of baculoviruses to infect and deliver exogenous genes to cells in this culture system. Efficient delivery to hepatocytes in short-term culture becomes restricted to peripheral cells, or "edge" cells, as the hepatocytes acquire intercellular junctions and form islands with time in culture. This barrier to baculovirus entry can be overcome, and the percentage of internal cells within the hepatocyte islands that are infected with the baculovirus can be increased more than 100-fold, when cells are subjected to transient calcium depletion before and during infection. These findings suggest that at least in some cell types, such as hepatocytes, baculovirus entry may require contact with the basolateral surface. We conclude from this study that recombinant baculovirus infection following transient depletion of extracellular calcium results in delivery of exogenous genes to at least 75% of hepatocytes in long-term DMSO culture, thereby making it possible for the first time to carry out gain-of-function and loss-of-function studies in this cell system.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Transient Disruption of Intercellular Junctions Enables Baculovirus Entry into Nondividing Hepatocytes

Bilello¹,

Delaney²,

Boyce

et al. 2001

J Virol

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“…Different studies have demonstrated that GP64 is essential for baculovirus transduction of mammalian cells (11). In mammalian cells, it has been shown that electrostatic interactions, heparan sulfate, and phospholipids are necessary for baculovirus binding to the mammalian cell surface (12).…”

mentioning

confidence: 99%

A Cholesterol Recognition Amino Acid Consensus Domain in GP64 Fusion Protein Facilitates Anchoring of Baculovirus to Mammalian Cells

Luz-Madrigal

Asanov²,

Camacho‐Zarco

et al. 2013

J Virol

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Baculoviridae is a large family of double-stranded DNA viruses that selectively infect insects. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the best-studied baculovirus from the family. Many studies over the last several years have shown that AcMNPV can enter a wide variety of mammalian cells and deliver genetic material for foreign gene expression. While most animal viruses studied so far have developed sophisticated mechanisms to selectively infect specific cells and tissues in an organism, AcMNPV can penetrate and deliver foreign genes into most cells studied to this date. The details about the mechanisms of internalization have been partially described. In the present study, we have identified a cholesterol recognition amino acid consensus (CRAC) domain present in the AcMNPV envelope fusion protein GP64. We demonstrated the association of a CRAC domain with cholesterol, which is important to facilitate the anchoring of the virus at the mammalian cell membrane. Furthermore, this initial anchoring favors AcMNPV endocytosis via a dynamin-and clathrin-dependent mechanism. Under these conditions, efficient baculovirus-driven gene expression is obtained. In contrast, when cholesterol is reduced from the plasma membrane, AcMNPV enters the cell via a dynamin-and clathrin-independent mechanism. The result of using this alternative internalization pathway is a reduced level of baculovirus-driven gene expression. This study is the first to document the importance of a novel CRAC domain in GP64 and its role in modulating gene delivery in AcMNPV.

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“…Although the specific interactions between baculovirus surface proteins and target cellular receptors are not exactly known, there is a hypothesis of virus binding via heparate sulphate residues 18 and phospholipids 19 supported by a large number of baculovirus-susceptible cell lines in vitro. 13 When considering the possible clinical use of baculoviruses, the transductional kinetics and suitable administration methods of baculoviruses in vivo have to be evaluated.…”

Section: Discussionmentioning

confidence: 99%

SPECT/CT imaging of baculovirus biodistribution in rat

et al. 2007

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Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells

Abstract: Electrostatic charges play a critical role during the first step in mammalian cell transduction mediated by a recombinant baculovirus.

Cited by 36 publications

References 27 publications

Transient Disruption of Intercellular Junctions Enables Baculovirus Entry into Nondividing Hepatocytes

Transient Disruption of Intercellular Junctions Enables Baculovirus Entry into Nondividing Hepatocytes

A Cholesterol Recognition Amino Acid Consensus Domain in GP64 Fusion Protein Facilitates Anchoring of Baculovirus to Mammalian Cells

SPECT/CT imaging of baculovirus biodistribution in rat

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