Badanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy — porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym

Kaczka, Krzysztof; Fendler, Wojciech; Borowiec, Maciej; Młynarski, Wojciech; Pomorski, Lech

doi:10.5603/ep.2014.0059

Cited by 10 publications

(3 citation statements)

References 42 publications

(48 reference statements)

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“…The OSNA technique was evaluated in two studies on thyroid cancer patients. Their results for the detection of sentinel lymph nodes were similar to those obtained using standard histopathology or molecular techniques [ 22 , 23 ]. The aim of the study is to determine the efficacy of OSNA and SLN biopsy in the intraoperative detection of nodal involvement.…”

Section: Introductionsupporting

confidence: 65%

One-step nucleic acid amplification analysis of sentinel lymph nodes in papillary thyroid cancer patients

Kaczka¹,

Pomorski²

2017

aoms

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IntroductionIt is essential to look for methods to define the need for central lymphadenectomy for papillary thyroid cancer patients. The aim is to determine the efficacy of one-step nucleic acid amplification (OSNA) and sentinel lymph node (SLN) biopsy in the intraoperative detection of nodal involvement.Material and methodsThis prospective, experimental study enrolled 49 patients with clinically negative lymph nodes. Intraoperatively, 1% Patent Blue dye was injected intratumorally. Lymph nodes that stained blue were defined as SLNs. They were directly cut into blocks at 2-mm intervals. Nonadjacent blocks were subjected to either the OSNA assay or histological examination.ResultsSixty-five SLNs were found in 43 (87.8%) patients. There were 20 (30.8%) histopathologically positive SLNs. According to the OSNA, 22 (33.8%) SLNs were positive. The OSNA results were different from histopathology in 8 (12.3%) SLNs. The OSNA gave a positive result in 5 (7.7%) SLNs, while they were not involved according to the histopathology. However, OSNA upstaged N status from N0 to N1 only in 2 (3.1%) patients. Inverse results (histopathology +, OSNA–) were obtained in 3 (4.6%) SLNs. Positive and negative predictive values (PPV and NPV) for OSNA were 0.77 and 0.93, respectively. The concordance rate between examinations was 85.5%.ConclusionsIn some patients with clinically negative lymph nodes, OSNA and SLN biopsy may prevent unnecessary central lymphadenectomy. On the other hand, the sentinel lymph node biopsy may reveal the presence of potentially involved sentinel lymph nodes outside the central compartment. These SLNs can also be assessed by means of OSNA.

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Section: Introductionsupporting

confidence: 65%

One-step nucleic acid amplification analysis of sentinel lymph nodes in papillary thyroid cancer patients

Kaczka¹,

Pomorski²

2017

aoms

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“…This is therefore the first study to examine the usefulness of SLNB-OSNA in this type of tumor. In a previous collaborative study, we found a good correlation between the conventional cytological and histological data and the OSNA technique in the analysis of lymph (24). The present study analyzes the results obtained with OSNA in application to SLNB in a group of 35 patients with PTC.…”

Section: Discussionmentioning

confidence: 69%

One-step nucleic acid amplification for intraoperative analysis of sentinel lymph node in papillary thyroid carcinoma

Felip

Llopis

Temprana-Salvador

et al. 2019

European Journal of Endocrinology

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Objective: Lymphadenectomy in papillary thyroid carcinoma (PTC) is controversial. It is indicated whenever metastases have been proven before or during surgery and as a prophylactic treatment in high-risk patients. However, 30-50% of cN0 patients become pN1 postoperatively. In PTC, selective-sentinel-lymph-node-biopsy (SLNB) with conventional intraoperative analysis is 8% false negative. One-step nucleic acid amplification (OSNA) is a molecular technique which allows real-time detection of mRNA encoding for cytokeratin 19. OSNA has been introduced in intraoperative analysis of several tumors to reduce false-negative rates and distinguish micrometastasis from macrometastasis. Our objective was to evaluate the impact of the introduction of OSNA in the intraoperative evaluation of the sentinel node (SN) in PTC. Design: We analyzed a series of 35 patients subjected to SLNB. Methods: All the dissected nodes, SN and non-SN, were evaluated with OSNA and cytology. Results: We obtained a total of 110 SN. SLNB proved positive in 14 patients (40%) with cytology and in 23 (65.7%) with OSNA (P < 0.001). In the 29 patients with subsequent lymphadenectomy we obtained 360 lymph nodes ((52 positive in cytology (14.4%) and 107 in OSNA (29.7%)). Lymphadenectomy proved positive in 16 patients according to cytology (55%) and in 24 according to OSNA (83%) (P = 0002). The majority of patients with micrometastasis in SN showed only micrometastasis in lymphadenectomy.Conclusions: The present study shows selective-sentinel-lymph-node-biopsy with one-step nucleic acid amplification technique to be feasible in papillary thyroid carcinoma. The quantitative nature of one-step nucleic acid amplification paves the way toward a more personalized surgical approach, limiting lymphadenectomy to patients with intraoperative evidence of macrometastasis in the sentinel node.

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“…54 articles did not have sufficient data for diagnostic testing. In summary, 61 articles involving 7 cancer types were included in the meta-analysis ( 9 , 10 , 13 – 71 ). Four cancer types, including bladder cancer, head and neck squamous cell carcinoma, ovarian cancer and pancreatic cancer, were not included for meta-analysis, due to limited literature.…”

Section: Resultsmentioning

confidence: 99%

Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis

Li,

Meng,

Zhang

et al. 2024

Front. Oncol.

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BackgroundThe status of the sentinel lymph nodes (SLNs) was an important prognostic factor in varies cancers. A one-step nucleic acid amplification (OSNA) assay, a molecular-based whole-node analysis method based on CK19 mRNA copy number, was developed to diagnose lymph node metastases. We aimed to evaluate the value of OSNA for the diagnosis of sentinel lymph node metastasis in CK19 positive cancers. CK19 mRNA and protein expression for pan-caner analysis were obtained from TCGA and the Human protein atlas database.MethodsTwo researchers independently searched the PubMed, Cochrane Library and Web of Science databases for qualified articles published before December 1, 2023. A meta-analysis was performed using MetaDisc and STATA. Risk bias and quality assessments of the included studies were evaluated, and a subgroup analysis was performed. Ten cancer types were found to be CK19 positively expressed and 7 of 10 had been reported to use OSNA for SLN detection.ResultsAfter literature review, there were 61 articles included in the meta-analysis, which consisted of 7115 patients with 18007 sentinel lymph nodes. The pooled sensitivity and specificity of OSNA were 0.87 and 0.95 in overall patients. Moreover, we found the background CK19 expression in normal tissue affected the diagnostic accuracy of OSNA. In breast cancer, we performed subgroup analysis. OSNA exhibited to be a stable method across different population groups and various medical centers. In addition, when 250 copies/μl was chosen as the cutoff point of CK19 mRNA, there were a relatively higher sensitivity and AUC in detecting SLN micro-metastasis than 5000 copies/μl.DiscussionOSNA can predict the occurrence of SLN metastasis accurately in CK19 positive cancers, especially in breast cancer, colorectal cancer, lung cancer, gastric cancer and endometrial cancer. Our study warrants future studies investigating the clinical application of OSNA in pancreatic, ovarian and bladder cancers.

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Badanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy — porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym

Cited by 10 publications

References 42 publications

One-step nucleic acid amplification analysis of sentinel lymph nodes in papillary thyroid cancer patients

One-step nucleic acid amplification analysis of sentinel lymph nodes in papillary thyroid cancer patients

One-step nucleic acid amplification for intraoperative analysis of sentinel lymph node in papillary thyroid carcinoma

Diagnostic value of one-step nucleic acid amplification for sentinel lymph node metastasis in cytokeratin 19-positive tumors: evidence from bioinformatics and meta-analysis

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