Badnaviruses: The Current Global Scenario

Bhat, Alangar Ishwara; Höhn, Thomas; Selvarajan, R.

doi:10.3390/v8060177

Cited by 154 publications

(114 citation statements)

References 162 publications

(257 reference statements)

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“…According to the Tenth International Committee on Taxonomy of Viruses (ICTV) report this family has been divided into six genera namely Caulimovirus, Petuvirus, Tungrovirus, Badnavirus, Soymovirus and Cavemovirus (Bhat et al, 2016;Geering and Hull, 2011). Viruses in this family encapsidate a circular dsDNA of about 7.5-8.0 Kbp which contains site-specific, single-stranded discontinuities generated during replication (Bhat et al, 2016;Geering and Hull, 2011). They express their genome as a greater than full-length RNA transcript following its entry into the cell nucleus.…”

Section: The Former Consists Of Viruses That Infect Legumes and Inclumentioning

confidence: 99%

“…Currently there are 32 recognised species within the genus Badnavirus, with another 20 awaiting formal recognition (Bhat et al, 2016). Badnaviruses are of great economic importance because they cause severe diseases in several important monocot and dicot crops.…”

Section: Badnavirusesmentioning

confidence: 99%

“…Badnaviruses are of great economic importance because they cause severe diseases in several important monocot and dicot crops. (Bhat et al, 2016). Many predicted ORFs have unknown functions, except for the large ORF III which encodes a polyprotein which is processed into functional units involved in replication, movement and encapsidation.…”

Section: Badnavirusesmentioning

confidence: 99%

“…The third cluster consists of the rest of the worldwide BSVs (Bhat et al, 2016). Currently there are nine distinctive species recognised by ICTV (Bhat et al, 2016). The double stranded DNA (dsDNA)…”

Section: Banana Streak Virusesmentioning

confidence: 99%

“…There are currently 70 complete genome sequences of 28 different BSV species and 20 complete genome sequences representing 16 putative species in the NCBI GenBank (Bhat et al, 2016).…”

Section: Banana Streak Virusesmentioning

confidence: 99%

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Assessment of Banana Streak MY virus-based infectious clone vectors in Musa ssp.

Onsarigo¹

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Banana streak disease, caused by a complex of different banana streak viruses (BSVs), occurs in all banana-producing countries. BSVs are genetically very diverse and can exist both as episomal forms or integrated into the host genome and both can be infectious to banana.Several field studies have assessed the infectivity of one or more BSV species on different banana genotypes with significant differences reported in virus incidence and symptom expression between and within genotypes. These studies used a severity index, based on symptom expression, to determine the resistance/susceptibility levels of the genotypes.Because of the great genetic variability amongst BSV species, symptoms of banana streak disease may vary widely and may be influenced by the cultivar and/or environmental conditions. Also this system of screening does not reflect the level of viral DNA accumulation In the current study, symptom variability was considerable with different Musa genotypes expressing distinct symptoms. Quantification of the levels of virus DNA showed that virus accumulation differs between genotypes, but also within plants of the same accession, and that severity scores do not correlate well with the viral load as measured using qPCR.Modified vectors with either ORF1 or ORF2 deleted were not infectious and the addition of a GFP ORF into two sites in the genome did not result in expression of this heterologous sequence from the constructs used in this study. Interestingly, two constructs with small inserts, initially designed for the purpose of gene silencing, were found to retain infectivity and maintain a heterologous sequence up to 110 nt. These studies confirm that BSMYV is infective in a broad range of Musa genotypes and cultivars but that further work is required to develop suitable vectors for either gene expression or gene silencing studies in banana

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Section: The Former Consists Of Viruses That Infect Legumes and Inclumentioning

confidence: 99%

Section: Badnavirusesmentioning

confidence: 99%

Section: Badnavirusesmentioning

confidence: 99%

Section: Banana Streak Virusesmentioning

confidence: 99%

“…There are currently 70 complete genome sequences of 28 different BSV species and 20 complete genome sequences representing 16 putative species in the NCBI GenBank (Bhat et al, 2016).…”

Section: Banana Streak Virusesmentioning

confidence: 99%

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Assessment of Banana Streak MY virus-based infectious clone vectors in Musa ssp.

Onsarigo¹

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Defense strategies against sweet potato chlorotic stunt and pakakuy virus coinfection unraveled

Kiemo,

Salamon,

Tóth

et al. 2024

Crop Science

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This study into the response of two Ipomoea batatas (L.) Lam cultivars, Melinda and Tio Joe, to coinfection with sweet potato chlorotic stunt virus (SPCSV) and sweet potato pakakuy virus (SPPV), employed a comprehensive approach encompassing symptomatology, real‐time quantitative polymerase chain reaction, metagenomics, and transcriptomics. SPCSV is a quarantine virus with synergistic effects, which decimate yields. SPPV is the most prevalent DNA virus in sweet potato germplasm, with a tendency to persist in meristems, posing a significant risk for germplasm transfer between territories. Graft inoculation experiments revealed that while Tio Joe remained asymptomatic for 12 weeks and suppressed virus replication, Melinda displayed symptoms early on and exhibited high virus titers. Metagenomic analyses corroborated these observations and confirmed that SPCSV and SPPV were responsible. Transcriptomic analysis unveiled disparities in gene expression between Melinda and Tio Joe. Differential gene expression was heightened and altered in Melinda as the viruses disrupted its gene expression. Its defense strategies, such as inducing abscisic acid signaling, were insufficient to overcome disruptive viral effects like oxidative stress, rendering it susceptible. In contrast, Tio Joe had relatively stable differential gene expression, indicating resistance to SPPV–SPCSV coinfection. Overexpressed genes such as sirtuin, rapid alkalization factor, and nuclear pore anchor triggered quantitative resistance, supported with enriched organelles such as mitochondrion and pathways such as proteasome and cutin, suberine, and wax biosynthesis. Tio Joe maintained its genome integrity and inhibited viral replication by tightly controlling gene expression and preventing reactive oxygen species accumulation.

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