Bai Hu Tang, Si Ni Tang, and Xue Bi Tang amplify pro-inflammatory activities and reduce apoptosis in endothelial cells in a cell culture model of sepsis

Brislinger, Dagmar; Daxböck, Christine; Roßmanith, Eva; Stückler, Manuela; Lang, Ingrid; Falkenhagen, D

doi:10.1016/j.jep.2018.07.021

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…HYJJ decoction encompasses ten herbs, and the combination of ACD, ZR and GF has been introduced as Si-Ni decoction carrying on a potential anti-inflammatory effect on the treatment of septic and shock sepsis (Chen et al, 2011;Brislinger et al, 2018;Zeng et al, 2019). However, the therapeutic role of HYJJ in UC has not yet been elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Breit (PB). Among these herbs, the combination of ACD, ZR and GF also termed by Si-Ni decoction has shown proven efficacy for septic shock (Chen et al, 2011), sepsis (Brislinger et al, 2018;Zeng et al, 2019) by interfering with apoptosis and inflammatory responses (Aly et al, 2005;Endo et al, 2017;Yang et al, 2017;Endo et al, 2018;Bai et al, 2020). In the context of SI-NI decoction, the anti-inflammatory function has been emphasized by the addition of PGM (Jin et al, 2020), PB (Tian et al, 2020), SCF (Panossian & Wikman, 2008;Li et al, 2020;Li et al, 2021), PCW as well as CC (Csikos et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Network Pharmacology-Based Validation of the Efficacy of Huiyangjiuji Decoction in the Treatment of Experimental Colitis

Cheng

Zhu

et al. 2021

Front. Pharmacol.

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Ulcerative colitis (UC) is the major type of inflammatory bowel disease (IBD) characterized by an overactive immune responses and destruction of the colorectal epithelium with intricate pathological factors. In China, Huiyangjiuji decoction (HYJJ) has been widely administered against inflammation, but the underlying mechanical mechanisms are not known. A murine model of colitis was established by orally feeding 4% dextran sodium sulfate for 5 days. Intestinal organoids (IOs) were treated with TNFα (Tumor necrosis factor-α) as an ex-vivo UC model. A scratch assay combined with a co-culture system that incubated murine epithelial cell line (IEC-6) with macrophages (Mφs) was utilized to assess epithelial recovery under inflammatory conditions. Network pharmacology analysis was performed to elucidate the mechanism of HYJJ decoction. In the present study, we confirmed that HYJJ considerably alleviated of DSS-induced colitis, as evidenced by the improved intestinal injury and fecal albumin, as well as feces blood. Network pharmacology analysis identified the active components in HYJJ formula, and KEGG enrichment analysis indicated that HYJJ-target genes were enriched in pathogen-induced infections, cancer-related as well as inflammatory pathways. Consistently, RNA-sequencing demonstrated that HYJJ treated inhibited cytokine-cytokine interaction, IBD as well as TNF signaling pathways, confirming the anti-inflammatory and anti-neoplastic role of HYJJ decoction. In-vitro experimental evidence confirmed the suppression of pro-interleukins by HYJJ, including IL-2, IL-10 and IL-12. Moreover, the contribution of HYJJ to mucosal healing was corroborated by ex-vivo experiments, in which HYJJ rescued TNFα-compromised IOs functions, i.e., elevated mitochondrial stress (MOS) and impaired regeneration capacity. IEC-6 cells co-culture with Mφs from HYJJ-treated experimental colitis mice showed an improved migration capacity as compared to those incubated with Mφs from untreated colitis mice. We conclude that HYJJ re-establishes homeostasis of the gut epithelium during colitis by suppressing inflammation and orchestrating cytokines interaction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Network Pharmacology-Based Validation of the Efficacy of Huiyangjiuji Decoction in the Treatment of Experimental Colitis

Cheng

Zhu

et al. 2021

Front. Pharmacol.

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“…According to bibliometrics analysis, we found the top 10 references. Plant immunity to insect herbivores [21] written by Howe GA was the most frequently cited, followed by Hormonal Modulation of Plant Immunity [22], Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack [23], Glucosinolate Metabolites Required for an Arabidopsis Innate Immune Response [24], Central memory self/tumor-reactive CD8(+) T cells confer superior antitumor immunity compared with effector memory T cells [25], Jasmonate Passes Muster: A Receptor and Targets for the Defense Hormone [26] Jasmonate passes muster: a receptor and targets for the defense hormone, et al In recent years, inflammation [27], oxidative stress [28], signal pathway [29], mechanism of action [30], regulatory T cells [31][32][33] have been the focus of research (Table 4). Bibliometrics method was used to analyze the development trend of TCM-Immune, and analyze the countries, institutions, authors, and magazines that have the most frequently published papers in this field and has important guiding significance for subsequent research.…”

Section: Discussionmentioning

confidence: 99%

Bibliometrics Research on Immune of Traditional Chinese Medicine

Bai¹,

Dong²,

Li³

et al. 2019

IJCM

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Bibliometrics analysis is increasingly used for situation analysis in a certain field. We aimed to analyze the immunological aspects of traditional Chinese medicine (TCM) from 1995 to the present, such as study countries, institutions, journals, and hotspots. The web of science core collection was used to retrieve the articles related to TCM-Immune from 1995 to 2018, and the statistical analysis of those projects by the Excel, VOS viewer software. A total of 4005 kinds of literature on TCM-Immune were retrieved. It was found that the total number of papers published in recent years had increased rapidly, with China being the country with the largest number of publications, followed by the United States, South Korea, India, and Japan. Top 10 institutions except Kyung-hee University are based in China, and so are the authors of the top three. VOS viewer software divided the keywords into four clusters, the first cluster is immune in vitro, the second cluster is immune in vivo, the third cluster is TCM-Immune resistance, the fourth cluster is the mechanism of immune, the analysis shows that inflammation, oxidative stress, signal pathway, the mechanism of action is new hotspots in the future.

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“…When looking for anti-inflammatory drugs to treat sepsis, mild natural compounds caught our attention. For example, Dagmar et al studied the effect and mechanism of Sini decoction, Baihu decoction, and Xuebi decoction in the treatment of sepsis endothelial cell model and found that the above traditional Chinese medicine has anti-inflammatory and antiapoptotic effects in sepsis [5]. It is well known that natural compounds containing β-glucan can be used to improve human health.…”

Section: Introductionmentioning

confidence: 99%

Effects of Lentinan on Endothelial Cell Activity, Inflammatory Response, Endoplasmic Reticulum Stress, and Apoptosis in Sepsis

2020

Advances in Polymer Technology

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The aim of this study is to explore the protective effects of lentinan on endoplasmic reticulum stress, inflammation, and apoptosis in sepsis endothelial cells. Firstly, lentinan was extracted, purified, and analyzed. When the concentration of lentinan was in the range of 0.04–4 μM, there was no obvious effect on the morphology of HUVECs. When the concentration reached 10 M, the cells were obviously contracted and necrotic. CCK-8 cell activity experiment showed that when the concentration of lentinan reached 4 μM, the cell activity decreased significantly (P<0.001), and it was in a dose-dependent manner. Then, the cells were divided into the control group (0 μM lentinan), sepsis group, sepsis + lentinan 1.2 μM group, and sepsis + lentinan 2 μM group. Enzyme-linked immunosorbent assay showed that lentinan could significantly reduce the expression of TNF-α, IL-1β, and IL-6 in sepsis endothelial cells (P<0.001). In addition, flow cytometry and TUNEL staining showed that compared with the control group, the apoptosis of cells in the sepsis group increased significantly (P<0.001), and lentinan could inhibit apoptosis (P<0.001). In terms of mechanism research, the mRNA and protein expression of endoplasmic reticulum stress-related protein in endothelial cells were detected by real-time fluorescent quantitative PCR (qPCR) and Western blotting, respectively. It was found that the expression of SIRT1, the upstream factors of endoplasmic reticulum stress in sepsis cells, was obviously inhibited (P<0.001), and the expression of CHOP, GRP78, IRE1α, and ATF6 was significantly increased (P<0.001), However, the pretreatment of lentinan could significantly reverse the above changes (P<0.001). Besides, lentinan could also reduce the expression of phosphorylated p65 protein (the activation marker of NF-κb) and iNOS. Conclusion. When sepsis occurs, lentinan can protect endothelial cells from ERS inflammation and apoptosis induced by sepsis. Thus, lentinan is expected to be a new target for the treatment of sepsis-induced endothelial damage.

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Bai Hu Tang, Si Ni Tang, and Xue Bi Tang amplify pro-inflammatory activities and reduce apoptosis in endothelial cells in a cell culture model of sepsis

Cited by 8 publications

References 32 publications

Network Pharmacology-Based Validation of the Efficacy of Huiyangjiuji Decoction in the Treatment of Experimental Colitis

Network Pharmacology-Based Validation of the Efficacy of Huiyangjiuji Decoction in the Treatment of Experimental Colitis

Bibliometrics Research on Immune of Traditional Chinese Medicine

Effects of Lentinan on Endothelial Cell Activity, Inflammatory Response, Endoplasmic Reticulum Stress, and Apoptosis in Sepsis

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