Baicalein inhibits the migration and invasive properties of human hepatoma cells

Chiu, Yung Wei; Lin, Taichen; Huang, Weiya; Teng, Chun Yuh; Liou, Yi Sheng; Kuo, Wu Hsien; Lin, Wea Lung; Huang, Hai I.; Tung, Jai Nien; Huang, Chih Yang; Liu, Jer Yuh; Wang, Wen Hung; Hwang, Jin Ming; Kuo, Hsing Chun

doi:10.1016/j.taap.2011.07.008

Cited by 100 publications

(98 citation statements)

References 42 publications

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“…In the present study, we demonstrated that baicalein effectively suppressed the overexpression of MMP-1, MMP-3 and MMP-13 in IL-1β-stimulated chondrocytes. Previous studies showed that baicalein reduced MMP-2 and MMP-9 expression in glioma and hepatocellular carcinoma cells [18,19]. In addition, MMP-1 was inhibited by baicalein in human keratinocytes [11].…”

Section: Discussionmentioning

confidence: 91%

Baicalein Inhibits MMPs Expression via a MAPK-Dependent Mechanism in Chondrocytes

Chen

Xiong

et al. 2015

Cell Physiol Biochem

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Background: Baicalein is a flavonoid isolated from Scutellaria baicalensis Georgi. Here, we investigated the anti-osteoarthritic effect of baicalein in vitro and in vivo. Methods: Interleukin-1 beta (IL-1β)-induced chondrocytes were treated with different concentrations of baicalein, real-time PCR and ELISA were performed to detect the matrix metalloproteinases (MMPs) expression. Western blot was used to evaluate the mitogen-activated protein kinase (MAPK) expression. In experimental osteoarthritis (OA), rabbits were treated with baicalein, gross morphological and histological assessment was performed to evaluate the cartilage damage. Results: Baicalein significantly reduced the expression of MMPs in vitro and in vivo. Moreover, baicalein significantly reduced the phosphorylation of p38 and extracellular signal regulated kinase (ERK), but not of c-Jun N-terminal kinase (JNK). In addition, intra-articular injection of baicalein ameliorated the cartilage damage in a rabbit model of OA induced by anterior cruciate ligament transection (ACLT). Conclusions: The results indicate that baicalein may be considered as a potential agent for OA treatment.

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Section: Discussionmentioning

confidence: 91%

Baicalein Inhibits MMPs Expression via a MAPK-Dependent Mechanism in Chondrocytes

Chen

Xiong

et al. 2015

Cell Physiol Biochem

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“…1), a purified flavonoid compound with defined chemical structure extracted from the dry roots of Scutellaria radix that is a broadly used herb in China, has been demonstrated that it has potential anti-tumor effects on many types of cancer cells, including HCC [4]. Previous studies have demonstrated that baicalein can inhibit the growth, invasion and metastasis of HCC through inducing cell cycle arrest and apoptosis, autophagy via several cancer-related signaling or molecules including MEK/ERK, JNK, Bcl-2, mTOR, CD24, 12-LOX, NF-κB [5-11]. MicroRNAs (miRNAs) are a large class of short non-coding RNAs, which paly critical roles in human carcinogenesis by mediating the post-transcriptional regulation of protein-coding genes, including HCC [12, 13], and accumulating evidence indicates that miRNAs may act as oncogenes or tumor suppressor genes and appear to be markedly diagnostic biomarkers and prognostic factors that exhibits strong potential as therapeutic molecules and targets for HCC treatment [14].…”

Section: Introductionmentioning

confidence: 99%

Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells

Bie

Sun

et al. 2017

Cell Physiol Biochem

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Background/Aims: Baicalein has been shown to possess significant anti-hepatoma activity by inhibiting cell proliferation. Whether the anti-proliferative effect of baicalein is related to its modulation of miRNA expression in hepatocellular carcinoma (HCC) is still unknown. Methods: The anti-proliferative effects of baicalein on HCC cell line Bel-7402 was assessed by detecting the proliferation activity, cell cycle distribution, expression changes of p21/CDKN1A, P27/CDKN1B, total Akt and phosphoryted AKT. Microarray analysis was conducted to determine the miRNA expression profiles in baicalein-treated or untreated Bel-7402 cells and then validated by qRT-PCR in two HCC cell lines (Bel-7402 and Hep3B). The gain-of-function of miR-3127-5p was performed by detecting anti-proliferative effects after transfecting miRNA mimics in cells. Finally, the expression level of miR-3127-5p in different HCC cell lines was determined by qRT-PCR. Results: Baicalein was able to inhibit the proliferation of Bel-7402 cells by inducing cell cycle arrest at the S and G2/M phase via up-regulating the expression of p21/CDKN1A and P27/CDKN1B and suppressing the PI3K/Akt pathway. Baicalein could alter the miRNA expression profiles in Bel-7402 cells. Putative target genes for differentially expressed miRNAs could be enriched in terms of cell proliferation regulation, cell cycle arrest and were mainly involved in MAPK, PI3K-Akt, Wnt, Hippo and mTOR signaling pathways. MiR- 3127-5p, one of up-regulated miRNAs, exhibits low expression level in several HCC cell lines and its overexpression could inhibit cell growth of Bel-7402 and Hep3B cell lines by inducing S phase arrest by up-regulating the expression of p21and P27 and repressing the PI3K/Akt pathway. Conclusions: Modulation of miRNA expression may be an important mechanism underlying the anti-hepatoma effects of baicalein.

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“…Baicalein and its glycoside baicalin, the major compounds in RS, were intensively reported for their antimigration and antiinvasion properties. Previous studies show that baicalein and baicalin are capable of inhibiting MMP-2 in human hepatoma cells, 28 breast cancer cells, 29 and endothelial cells. 30 Suppression of MMP-2 contributes to the antiinvasiveness of baicalein and baicalin.…”

Section: Discussionmentioning

confidence: 97%

A Chinese Medicine Formula Gegen Qinlian Decoction Suppresses Expansion of Human Renal Carcinoma With Inhibition of Matrix Metalloproteinase-2

et al. 2014

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Aim of Study. Gegen Qinlian decoction (GQLD) is an ancient Chinese medicine formula for treating diseases with inner heat. The aim of this study is to investigate the antitumor effect of GQLD in human renal carcinoma cell (RCC) and its possible mechanism. Method. High-performance liquid chromatography was used to identify and quantify active compounds in GQLD. Inhibition of tumor growth was determined by xenograft model. Cell viability on treatment with the decoction was determined by MTT assay; quantitative real-time polymerase chain reaction and immunoblotting were used to determine gene and protein expression; matrix metalloproteinase-2 (MMP-2) activity was detected by gelatin zymography and in vitro enzymatic reaction assay. Results. Thirteen major peaks were detected in the decoction, 8 of which were identified as berberine, baicalin and baicalein, pueranin, daizidin, liquiritin, wogonoside, and wogonin. GQLD exhibited potent inhibition on xenografted expansion of RCC cells. Interestingly, GQLD treatment did not induce cell death to RCC cells, but blocked the neoangiogenesis in xenografted RCC tumor. Particularly, we found that GQLD significantly inhibited MMP-2 in RCC cells, which was involved as a critical factor in avascular growth of RCC. GQLD directly suppressed the enzyme activity of MMP-2. Radix Scutellariae was the major herbal component that contributed to the potent inhibition of MMP-2. Conclusion. The findings of this study provide experimental evidence of the inhibition of expansion and neoangiogenesis of renal carcinoma by Chinese medicine formula GQLD with involvement of MMP-2 suppression.

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Baicalein inhibits the migration and invasive properties of human hepatoma cells

Cited by 100 publications

References 42 publications

Baicalein Inhibits MMPs Expression via a MAPK-Dependent Mechanism in Chondrocytes

Baicalein Inhibits MMPs Expression via a MAPK-Dependent Mechanism in Chondrocytes

Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells

A Chinese Medicine Formula Gegen Qinlian Decoction Suppresses Expansion of Human Renal Carcinoma With Inhibition of Matrix Metalloproteinase-2

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