Baicalin attenuates angiotensin II-induced blood pressure elevation and modulates MLCK/p-MLC signaling pathway

Liu, Huixin; Cheng, Ying; Chu, Jianfeng; Yan, Mengchao; Wang, Di; Xie, Qiurong; Ali, Farman; Fang, Yi; Wei, Lihui; Yang, Yanyan; Shen, Aling; Peng, Jun

doi:10.1016/j.biopha.2021.112124

Cited by 18 publications

(11 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Special neurons in the retina react to light and transmit it to the brain to produce vision ( Pozarickij et al, 2020 ; Creeden et al, 2021 ; Liu H. et al, 2021 ; Yu et al, 2021 ; Elkhalifa et al, 2022 ). When neurons in the retina stop working properly, the eye can’t work properly.…”

Section: Nervous System-based Clinical Researchmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

View full text Add to dashboard Cite

Optogenetic is a technique that combines optics and genetics to control specific neurons. This technique usually uses adenoviruses that encode photosensitive protein. The adenovirus may concentrate in a specific neural region. By shining light on the target nerve region, the photosensitive protein encoded by the adenovirus is controlled. Photosensitive proteins controlled by light can selectively allow ions inside and outside the cell membrane to pass through, resulting in inhibition or activation effects. Due to the high precision and minimally invasive, optogenetics has achieved good results in many fields, especially in the field of neuron functions and neural circuits. Significant advances have also been made in the study of many clinical diseases. This review focuses on the research of optogenetics in the field of neurobiology. These include how to use optogenetics to control nerve cells, study neural circuits, and treat diseases by changing the state of neurons. We hoped that this review will give a comprehensive understanding of the progress of optogenetics in the field of neurobiology.

show abstract

Section: Nervous System-based Clinical Researchmentioning

confidence: 99%

The Roles of Optogenetics and Technology in Neurobiology: A Review

Chen

Liang

et al. 2022

Front. Aging Neurosci.

View full text Add to dashboard Cite

show abstract

“…45 As studied previously, pharmacological molecules could intervene in restricting the cell cycle events and proliferation of VSMCs. 16,44,46 Thus, inhibition of cell cycle progression has been considered a promising approach for controlling VSMCs proliferation induced by Ang II. Flow cytometry was employed to measure the proportion of cells with the respective cell cycle phase, that is, G0/G1, S, and G2/M phases.…”

Section: Discussionmentioning

confidence: 99%

“…The required lengths of fragmented mRNA were obtained from purified mRNA as given in our previous studies. 16 Next, cDNA was purified with AMPure XP beads (144 μL), followed by end repair/ dAtail, adaptor ligation, and PCR amplification. Furthermore, output libraries were evaluated utilizing a KAPA library quantification kit on an Agilent 2100 Bioanalyzer.…”

Section: Rna Sequencing and Enrichment Analysismentioning

confidence: 99%

See 1 more Smart Citation

Quercetin attenuates angiotensin II‐induced proliferation of vascular smooth muscle cells and p53 pathway activation in vitro and in vivo

et al. 2023

Self Cite

View full text Add to dashboard Cite

Quercetin is an essential flavonoid mostly found in herbal plants, fruits, and vegetables, which exhibits anti‐hypertension properties. However, its pharmacological impact on angiotensin II (Ang II) induced the increase of blood pressure along with in‐depth mechanism needs further exploration. The present study pointed out the anti‐hypertensive role of quercetin and its comprehensive fundamental mechanisms. Our data showed that quercetin treatment substantially reduced the increase in blood pressure, pulse wave velocity, and aortic thickness of abdominal aorta in Ang II‐infused C57BL/6 mice. RNA sequencing revealed that quercetin treatment reversed 464 differentially expressed transcripts in the abdominal aorta of Ang II‐infused mice. Moreover, overlapping KEGG‐enriched signaling pathways identified multiple common pathways between the comparison of Ang II versus control and Ang II + quercetin versus Ang II. Likewise, these pathways included cell cycle as well as p53 pathways. Transcriptome was further validated by immunohistochemistry, indicating that quercetin treatment significantly decreased the Ang II‐induced expression of proliferating cell nuclear antigen (PCNA), cyclin‐dependent kinase‐4 (CDK4), and cyclin D1, while increased protein expression of p53, and p21 in abdominal aortic tissues of mice. In vitro, quercetin treatment meaningfully decreased the cell viability, arrested cell cycle at G0/G1 phase, and up‐regulated the p53 and p21 proteins expression, as well as down‐regulated the protein expression of cell cycle‐related markers, for example, CDK4, cyclin D1 in Ang II stimulated vascular smooth muscle cells (VSMCs). This study addresses pharmacologic and mechanistic perspectives of quercetin against Ang‐II‐induced vascular injury and the increase of blood pressure.

show abstract

“…For the extraction of total proteins, Western cell lysis buffer (Beyotime Biotechnology, Shanghai, China) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and other protease inhibitors including cocktail (MedChemExpress, Monmouth Junction, NJ, United States), and phosStop (Roche; Basel, Switzerland) was used. Briefly, cells were lysed with the lysis buffer for 20 min in ice and centrifuged at 14,000 g at 4°C for 20 min as described previously ( Liu et al, 2021 ). Followed by the collection of supernatants, total concentration of protein was assessed by BCA protein kit assay and equal amount of each sample were subjected for separation through 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).…”

Section: Methodsmentioning

confidence: 99%

Quercetin inhibits angiotensin II-induced vascular smooth muscle cell proliferation and activation of JAK2/STAT3 pathway: A target based networking pharmacology approach

et al. 2022

View full text Add to dashboard Cite

The rapid growth of vascular smooth muscle cells (VSMCs) represents crucial pathological changes during the development of hypertensive vascular remodeling. Although quercetin exhibits significantly therapeutic effects on antihypertension, the systematic role of quercetin and its exact mode of action in relation to the VSMCs growth and its hypertension-related networking pharmacology is not well-documented. Therefore, the effect of quercetin was investigated using networking pharmacology followed by in vitro strategies to explore its efficacy against angiotensin II (Ang II)-induced cell proliferation. Putative genes of hypertension and quercetin were collected using database mining, and their correlation was investigated. Subsequently, a network of protein-protein interactions was constructed and gene ontology (GO) analysis was performed to identify the role of important genes (including CCND1) and key signaling pathways [including cell proliferation and Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway]. We therefore further investigated the effects of quercetin in Ang II-stimulated VSMCs. This current research revealed that quercetin significantly reduced the cell confluency, cell number, and cell viability, as well as expression of proliferating cell nuclear antigen (PCNA) in Ang II-stimulated VSMCs. Mechanistic study by western blotting confirmed that quercetin treatment attenuated the activation of JAK2 and STAT3 by reducing its phosphorylation in Ang II stimulated VSMCs. Collectively, the current study revealed the inhibitory effects of quercetin on proliferation of Ang II stimulated VSMCs, by inhibiting the activation of JAK2/STAT3 signaling might be one of underlying mechanisms.

show abstract

Baicalin attenuates angiotensin II-induced blood pressure elevation and modulates MLCK/p-MLC signaling pathway

Cited by 18 publications

References 48 publications

The Roles of Optogenetics and Technology in Neurobiology: A Review

The Roles of Optogenetics and Technology in Neurobiology: A Review

Quercetin attenuates angiotensin II‐induced proliferation of vascular smooth muscle cells and p53 pathway activation in vitro and in vivo

Quercetin inhibits angiotensin II-induced vascular smooth muscle cell proliferation and activation of JAK2/STAT3 pathway: A target based networking pharmacology approach

Contact Info

Product

Resources

About