Baicalin induced colon cancer cells apoptosis through miR-217/DKK1-mediated inhibition of Wnt signaling pathway

Yan-mei, Jia; Chen, Lirong; Guo, Songjia; Li, Yuanhong

doi:10.1007/s11033-019-04618-9

Cited by 45 publications

(24 citation statements)

References 16 publications

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“…However, it is worth noting that in the present study, the apoptotic rate of the NG group was >10%, which may just represent the normal rate of programmed cell death in podocytes or it may be due to the cell culture environment; thus, further confirmation is required. Another interesting result was that in contrast with our study, a previous study reported that BA induced apoptosis in cancer cells (10)(11)(12).…”

Section: Discussioncontrasting

confidence: 99%

“…Baicalin (BA; 5,6,7-trihydroxyflavone), a herbal medicine found in the Chinese Pharmacopoeia, is one of the main flavonoids purified from the roots of Astragalus membranaceus (9). It has been reported that BA exhibits significant anticancer effects in a variety of malignant tumors, including colorectal (10), breast (11) and lung cancer (12), where it served an important role in regulating cell growth. In addition, in a previous study, it was identified that BA treatment improved the renal function of patients with DN and delayed the progression of DN through both anti-inflammatory and antioxidation mechanisms (13).…”

Section: Introductionmentioning

confidence: 99%

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Baicalin serves a protective role in diabetic nephropathy through preventing high glucose‑induced podocyte apoptosis

Ling

Yin³

et al. 2020

Exp Ther Med

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Diabetic nephropathy (DN) is one of the late complications of diabetes, which seriously affects the lives of patients. Baicalin (BA) is a flavone glycoside that has been identified to improve renal function in patients with DN. The present study aimed to investigate the roles and mechanisms of BA in DN. For that purpose, podocytes were cultured for 48 h under conditions of high glucose (HG; 30 mM D-glucose) or normal glucose (NG; 5 mM D-glucose). Then, the cells were treated with different concentrations of BA (6.25, 12.5 and 25 µM) for 24 h. Cell viability and apoptosis were determined using an MTT assay and flow cytometry, respectively. Protein and mRNA expression levels were analyzed using western blotting and reverse transcription-quantitative PCR, respectively. BA treatment was identified to promote the viability of podocytes and suppress cell apoptosis in a dose-dependent manner. Compared with the results in the NG group, HG stimulation significantly decreased the viability of podocytes and increased the apoptotic rate, whereas BA treatment following HG stimulation increased the viability of podocytes and decreased the apoptotic rate. Moreover, the effect of BA was revealed to be associated with the sirtuin 1/NF-κB signaling pathway in DN. In conclusion, the results of the present study suggested that BA treatment may significantly decrease HG-induced podocyte apoptosis, which indicated that BA might be a promising agent for DN treatment.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Baicalin serves a protective role in diabetic nephropathy through preventing high glucose‑induced podocyte apoptosis

Ling

Yin³

et al. 2020

Exp Ther Med

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“…In these miRNAs, miR-217 and miR-144 are upregulated, miR-129, miR-125a, miR-125b, miR-375, miR-328, miR-486, and miR-194 are downregulated. In COAD, miR-217 specifically inhibits DKK1, which is an important antagonist of the Wnt signaling pathway to promote apoptosis of colon cells (Jia et al, 2019). By controlling the expression of SMAD4, miR-144 inhibits invasion FIGURE 3 | The GO analysis (A) and KEGG pathway analysis (B) of common genes.…”

Section: Identification Of Mirna With Prognostic Value In Coadmentioning

confidence: 99%

MicroRNAs Associated With Colon Cancer: New Potential Prognostic Markers and Targets for Therapy

Zhu

Xü

Liu

et al. 2020

Front. Bioeng. Biotechnol.

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MicroRNAs (miRNAs) are a kind of non-coding RNA (ncRNA) that regulate the expression of target genes and play a role in the occurrence and development of cancers. Colon cancer (COAD) is the second most common cause of cancer-related mortality. However, the prognostic value of miRNAs in COAD is still confusing. In this study, we obtain miRNAs and messenger RNAs (mRNAs) expression profiles of COAD from the Cancer Genome Atlas (TCGA) database. After preliminary data screening and preprocessing, we acquire the expression data of 894 miRNAs and 17,019 mRNAs. Then, compared with the normal samples, 39 upregulated miRNAs and 54 downregulated miRNAs are identified by differential expression analysis. Furthermore, we obtain 1,487 upregulated mRNAs and 2,847 downregulated mRNAs. We confirm nine key miRNAs related to the survival rate of COAD patients. Moreover, by using bioinformatics methods, we get 461 common genes from both the target genes of these nine key miRNAs and differentially expressed mRNAs. Through analyzing the protein-protein interaction (PPI) network of these 461 common genes and survival analysis, we confirm five hub genes as promising biomarkers for COAD prognosis. It is worth mentioning that no previous reports have found that PGR and KCNB1 are related to COAD. We expect these key miRNAs and hub genes will provide a new way for the study of COAD.

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“…Apoptotic cell death is induced by baicalin treatments, and further evidence showed that various oncogenic miRNAs, such as miR-23 and miR-217, can be depleted by baicalin in colorectal cancer [ 36 , 99 ] ( Figure 2 and Table 3 ). Baicalin impedes the growth of colorectal cancer in vivo via repressing c-Myc expression in conjunction with a reduction of miR-23, which is one of the c-Myc-regulated miRNAs [ 36 ].…”

Section: Oncogenic Mirnas Inhibited By Phytochemicals Currently Evmentioning

confidence: 99%

“…Baicalin impedes the growth of colorectal cancer in vivo via repressing c-Myc expression in conjunction with a reduction of miR-23, which is one of the c-Myc-regulated miRNAs [ 36 ]. Additionally, baicalin can induce apoptosis through subduing Wnt/β-catenin signaling, accompanied by the upregulated levels of Dickkopf-related protein 1 (DKK1), an endogenous inhibitor of Wnt signaling [ 99 ]. In this study, it was noted that baicalin downregulates the expression of miR-217, which undeviatingly targets DKK1.…”

Section: Oncogenic Mirnas Inhibited By Phytochemicals Currently Evmentioning

confidence: 99%

Participation of MicroRNAs in the Treatment of Cancer with Phytochemicals

et al. 2020

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Cancer is a global health concern and one of the main causes of disease-related death. Even with considerable progress in investigations on cancer therapy, effective anti-cancer agents and regimens have thus far been insufficient. There has been compelling evidence that natural phytochemicals and their derivatives have potent anti-cancer activities. Plant-based anti-cancer agents, such as etoposide, irinotecan, paclitaxel, and vincristine, are currently being applied in medical treatments for patients with cancer. Further, the efficacy of plenty of phytochemicals has been evaluated to discover a promising candidate for cancer therapy. For developing more effective cancer therapy, it is required to apprehend the molecular mechanism deployed by natural compounds. MicroRNAs (miRNAs) have been realized to play a pivotal role in regulating cellular signaling pathways, affecting the efficacy of therapeutic agents in cancer. This review presents a feature of phytochemicals with anti-cancer activity, focusing mainly on the relationship between phytochemicals and miRNAs, with insights into the role of miRNAs as the mediators and the regulators of anti-cancer effects of phytochemicals.

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Baicalin induced colon cancer cells apoptosis through miR-217/DKK1-mediated inhibition of Wnt signaling pathway

Cited by 45 publications

References 16 publications

Baicalin serves a protective role in diabetic nephropathy through preventing high glucose‑induced podocyte apoptosis

Baicalin serves a protective role in diabetic nephropathy through preventing high glucose‑induced podocyte apoptosis

MicroRNAs Associated With Colon Cancer: New Potential Prognostic Markers and Targets for Therapy

Participation of MicroRNAs in the Treatment of Cancer with Phytochemicals

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