Baicalin Inhibits Human Cervical Cancer Cells by Suppressing Protein Kinase C/Signal Transducer and Activator of Transcription (PKC/STAT3) Signaling Pathway

Abstract

BackgroundLike other human cancers, the malignancy of cervical cancer is also characterized by abilities of proliferation, migration, and invasion. Protein kinase C-zeta (PKCζ) has been highly correlated with several human cancers. Baicalin was proven to regulate PKC. This study aimed to investigate the anti-cancer effect and involved molecular mechanisms of baicalin on human cervical cancer.Material/MethodsBaicalin at various concentrations was used to treat 2 human cervical cancer cell lines HeLa and SiHa. The proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenylterazolium bromide (MTT) assay. The apoptosis was detected by terminal transferase UTP nick end labeling (TUNEL) assay. Wound healing assay and Transwell assay were used to evaluate the migration and invasion respectively. Western blotting was performed to assess the protein expression levels.ResultsBaicalin administration significantly reduced the viability by facilitating the apoptosis in HeLa and SiHa cells. Baicalin treatment also significantly reduced the wound closure and cell amount invaded as measured by Transwell assay. The expression levels of PKCζ, survivin, matrix metalloproteinase (MMP)2, MMP9 as well as the phosphorylation of signal transducer and activator of transcription (STAT) 3 were reduced in baicalin administrated cervical cancer cells.ConclusionsBaicalin exerted anti-cancer effects on human cervical cancer cells by targeting STAT3 regulated signaling pathways.

In vitro and in vivo activities of flavonoids – apigenin, baicalin, chrysin, scutellarin – in regulation of hypertension – a review for their possible effects in pregnancy-induced hypertension

“…The antiinflammatory activity of baicalin was also found in other cell cultures i.e. human arterial endothelial cells (HAECs) exposed to ischemia reperfusion injury [63], human chondrocyte cell line CHON-001 treated with IL-1β [64], human hepatocyte cell lβe [66], human cervical cancer cell lines HeLa and SiHa [67].…”

“…So far, only one study on the effect of baicalin in preeclampsia (PE) has been carried out. Wang et al [67] pointed to the protective role of baicalin during injury of liver and kidney caused by PE in rat model. Baicalin were treated pregnant rats with PE in tree following doses: 50, 100, and 150 mg/kg/day, which were given by the intraperitoneal injection for 20 days.…”

Surfactin from Bacillus subtilis attenuates ambient air particulate matter-promoted human oral cancer cells metastatic potential

“…Therefore, reducing the expression of MMP-2 and MMP-9 or its upstream regulatory signaling pathways is essential for the treatment of malignant tumors. Up-regulation of MMP-2 and MMP-9 expression was mediated through various signaling pathways [8,9]. Here, we studied the novel pathways involved in PM-mediated MMP-2 and MMP-9 expression in the SCC4 and SCC25 human oral squamous cell carcinoma cell lines.…”

<p>Baicalin Ameliorates Dexamethasone-Induced Osteoporosis by Regulation of the RANK/RANKL/OPG Signaling Pathway</p>

“…21 Baicalin (BA), also known as 7-glucuronic acid-5,6-dihydroxy-flavone, is a flavonoid isolated from Scutellaria baicalensis Georgi (Lamiaceae) and is broadly used as a herbal treatment for various inflammatory diseases in traditional Chinese medicine. 22,23 Previous studies revealed that BA exhibits widely potent biological activities, including anti-inflammatory, 24 antiviral 25 and antitumor effects, 26 etc. BA has been reported to suppress inflammation by inhibiting NF-κB, 27,28 which plays an essential role in the differentiation and function of osteoclasts.…”