Baicalin promotes antiviral IFNs production and alleviates type I IFN-induced neutrophil inflammation

Li, Li; Dong, Jie; Ye, H.; Jiang, Man-Jing; Yang, Haisong; Liang, Linda; Ning, Lei; Wu, Ying

doi:10.1007/s11418-023-01702-0

Cited by 7 publications

(2 citation statements)

References 23 publications

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“…According to our findings, cisplatin treatment significantly increased the TNF-α, IL-6, IL-1β, and NO contents in the MDCK cells, indicating the occurrence of cellular inflammatory injury. Baicalin effectively repaired the inflammatory damage caused through cisplatin by reducing inflammatory factor levels, and these results were similar to those of previous research [48,49]. This suggests that baicalin, in ameliorating cisplatin-induced MDCK cell injury, not only improves oxidative stress but also exerts immunomodulatory effects.…”

Section: Discussionsupporting

confidence: 89%

Baicalin Exhibits a Protective Effect against Cisplatin-Induced Cytotoxic Damage in Canine Renal Tubular Epithelial Cells

Wang,

Li,

Yan

et al. 2023

Metabolites

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Renal failure is a common chronic disease in dogs that substantially affects both their quality of life and longevity. The objective of this study was to assess the protective mechanisms of baicalin in cisplatin-induced Madin–Darby canine kidney (MDCK) epithelial cells’ apoptosis model and explore the impacts of baicalin at varying doses on various indexes, such as cisplatin-induced MDCK cell apoptosis, oxidation and antioxidation, and inflammatory factors. (Methods) MDCK cells in the logarithmic growth phase were randomly divided into a control group, a model group (20 μmol/L cisplatin), and a baicalin-protection group (20 μmol/L cisplatin + 50, 25 μmol/L baicalin) and received the corresponding treatments for 24 h. The effects of cisplatin on MDCK cell apoptosis, oxidation and antioxidation, inflammatory factors, and other indicators were studied, and the relieving effect of baicalin on cisplatin-induced MDCK cell damage was explored. Calcein/PI staining and Annexin V-FITC/PI staining showed that cisplatin induced the apoptosis of MDCK cells, while baicalin effectively reduced the damage caused by cisplatin. The ELISA results demonstrated a significant elevation in the nitric oxide (NO) and malondialdehyde (MDA) levels within the MDCK cells following treatment with cisplatin (p < 0.01). In addition, superoxide dismutase (SOD), glutathione peroxidase (GSH), and catalase (CAT) activities remarkably declined (p < 0.01), while tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) expression within the MDCK cells were apparently elevated (p < 0.01). However, baicalin treatment resulted in opposite changes in these factors. The findings suggested that baicalin exhibits potential in mitigating cisplatin-induced oxidative stress and inflammation in MDCK cells. As revealed with the Western blot results, cisplatin promoted P62, P53, and BAX protein levels, increased mTOR phosphorylation, inhibited AMPK phosphorylation, and reduced Beclin1 and BCL-2 protein levels. However, a contrasting trend was observed following baicalin treatment. Cisplatin can inhibit the activity of MDCK cells, lead to abnormalities in oxidation and antioxidation functions and cell inflammatory factors, and accelerate cell apoptosis. Moreover, baicalin can significantly alleviate the damage of cisplatin to MDCK cells.

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Section: Discussionsupporting

confidence: 89%

Baicalin Exhibits a Protective Effect against Cisplatin-Induced Cytotoxic Damage in Canine Renal Tubular Epithelial Cells

Wang,

Li,

Yan

et al. 2023

Metabolites

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“…The metabolism of amino acids in the body is intricately linked to the extent of inflammation damage. Bai can regulate arginine biosynthesis and alleviate inflammation damage induced by MDR Pseudomonas aeruginosa in acute pneumonia in rats [41]. The pathway enrichment analysis in our study revealed that Bai treatment has specific effects on the amino acid metabolism (Tyrosine, Phenylalanine, Tyrosine and Tryptophan) and steroid hormone biosynthesis.…”

Section: Discussionmentioning

confidence: 59%

Baicalin Protects Broilers against Avian Coronavirus Infection via Regulating Respiratory Tract Microbiota and Amino Acid Metabolism

Feng,

Zhang,

Wang

et al. 2024

IJMS

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An increasing amount of evidence indicates that Baicalin (Bai, a natural glycosyloxyflavone compound) exhibits an antiviral effect against avian viruses. However, it remains unclear if the antiviral effect of Bai against infectious bronchitis virus (IBV) is exerted indirectly by modulating respiratory tract microbiota and/or their metabolites. In this study, we investigated the protection efficacy of Bai in protecting cell cultures and broilers from IBV infection and assessed modulation of respiratory tract microbiota and metabolites during infection. Bai was administered orally to broilers by being mixed in with drinking water for seven days. Ultimately, broilers were challenged with live IBV. The results showed that Bai treatment reduced respiratory tract symptoms, improved weight gain, slowed histopathological damage, reduced virus loads and decreased pro-inflammation cytokines production. Western blot analysis demonstrated that Bai treatment significantly inhibited Toll-like receptor 7 (TLR7), myeloid differentiation factor 88 (MyD88) and nuclear factor kappa-B (NF-κB) expression both in cell culture and cells of the trachea. Bai treatment reversed respiratory tract microbiota dysbiosis, as shown by 16S rDNA sequencing in the group of broilers inoculated with IBV. Indeed, we observed a decrease in Proteobacteria abundance and an increase in Firmicutes abundance. Metabolomics results suggest that the pentose phosphate pathway, amino acid and nicotinamide metabolism are linked to the protection conferred by Bai against IBV infection. In conclusion, these results indicated that further assessment of anti-IBV strategies based on Bai would likely result in the development of antiviral molecule(s) which can be administered by being mixed with feed or water.

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Interferon-I modulation and natural products: Unraveling mechanisms and therapeutic potential in severe COVID-19

Song,

Lu,

Qin

et al. 2024

Cytokine & Growth Factor Reviews

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Baicalin promotes antiviral IFNs production and alleviates type I IFN-induced neutrophil inflammation

Cited by 7 publications

References 23 publications

Baicalin Exhibits a Protective Effect against Cisplatin-Induced Cytotoxic Damage in Canine Renal Tubular Epithelial Cells

Baicalin Exhibits a Protective Effect against Cisplatin-Induced Cytotoxic Damage in Canine Renal Tubular Epithelial Cells

Baicalin Protects Broilers against Avian Coronavirus Infection via Regulating Respiratory Tract Microbiota and Amino Acid Metabolism

Interferon-I modulation and natural products: Unraveling mechanisms and therapeutic potential in severe COVID-19

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