Baicalin protects PC-12 cells from oxidative stress induced by hydrogen peroxide via anti-apoptotic effects

Zheng, Wenxia; Wang, Feng; Cao, Xiaolu; Pan, Hong-yan; Li, Xiaoying; Hu, Xingjian; Sun, Yuanyuan

doi:10.3109/02699052.2013.860469

Cited by 61 publications

(29 citation statements)

References 25 publications

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“…Previous in vitro and in vivo studies clearly demonstrated the beneficial effects of BCN in different models of oxidative/nitrosative stress and inflammation. BCN reduced the generation of ROS, RNS and inflammatory cytokines, inhibited membrane lipid peroxidation and attenuated tissue necrosis and apoptosis (Xu et al ., ; Lin et al ., ; Zheng et al ., ; Wei et al ., ). A recent in vitro study showed that BCN significantly decreased sertoli cells apoptosis induced by heat stress (Guo et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Dose-dependent protective effect of baicalin against testicular torsion-detorsion in rats

2016

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Testicular torsion/detorsion induces oxidative/nitrosative stress, inflammation and apoptosis of testicular tissues. Baicalin exerts antioxidant and anti-inflammatory properties. This study investigated the possible protective effect of baicalin against testicular torsion-detorsion injury in rats. Surgical testicular torsion was induced for 2 h, followed by detorsion which was continued for 24 h. Baicalin was administered in three different doses (25, 50 and 100 mg kg , by intraperitoneal injection). Each dose was given twice, the first 30 min before and the second 12 h after testicular detorsion. Baicalin, in a dose-dependent manner, decreased the torsion/detorsion-induced elevations of testicular malondialdehyde, nitric oxide, tumour necrosis factor-α, BCL2-associated X protein (Bax), cytosolic cytochrome c and caspase-3 and caspase-9 activities. Baicalin, dose dependently, attenuated the reductions of B-cell leucemia/lymphoma 2 (Bcl-2), and glutathione peroxidase and superoxide dismutase activities in testicular tissues resulted from torsion/detorsion. In addition, baicalin ameliorated the histopathological testicular tissue damage and reduced the expression of Fas ligand in rat testes exposed to torsion/detorsion in a dose-dependent manner. It was concluded that baicalin, dose dependently, ameliorated testicular injury induced by torsion/detorsion via its antioxidant, antinitrosative, anti-inflammatory and anti-apoptotic effects.

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Section: Introductionmentioning

confidence: 99%

Dose-dependent protective effect of baicalin against testicular torsion-detorsion in rats

2016

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“…H 2 O 2 is produced at nearly every stage of enzymatic oxidase action and applied widely as an inducer of neuronal oxidative stress in vitro (Zheng et al 2014;Zhang et al 2012). Since NADPH oxidase is a very important enzyme that contributes to ROS generation in CNS, propofol is reported to inhibit Ca 2?…”

Section: Introductionmentioning

confidence: 99%

Propofol Protects Against H2O2-Induced Oxidative Injury in Differentiated PC12 Cells via Inhibition of Ca2+-Dependent NADPH Oxidase

et al. 2015

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Propofol (2,6-diisopropylphenol) is a widely used general anesthetic with anti-oxidant activities. This study aims to investigate protective capacity of propofol against hydrogen peroxide (H2O2)-induced oxidative injury in neural cells and whether the anti-oxidative effects of propofol occur through a mechanism involving the modulation of NADPH oxidase (NOX) in a manner of calcium-dependent. The rat differentiated PC12 cell was subjected to H2O2 exposure for 24 h to mimic a neuronal in vitro model of oxidative injury. Our data demonstrated that pretreatment of PC12 cells with propofol significantly reversed the H2O2-induced decrease in cell viability, prevented H2O2-induced morphological changes, and reduced the ratio of apoptotic cells. We further found that propofol attenuated the accumulation of malondialdehyde (biomarker of oxidative stress), counteracted the overexpression of NOX core subunit gp91(phox) (NOX2) as well as the NOX activity following H2O2 exposure in PC12 cells. In addition, blocking of L-type Ca(2+) channels with nimodipine reduced H2O2-induced overexpression of NOX2 and caspase-3 activation in PC12 cells. Moreover, NOX inhibitor apocynin alone or plus propofol neither induces a significant downregulation of NOX activity nor increases cell viability compared with propofol alone in the PC12 cells exposed to H2O2. These results demonstrate that the protective effects of propofol against oxidative injury in PC12 cells are mediated, at least in part, through inhibition of Ca(2+)-dependent NADPH oxidase.

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“…It could be considered possible that martynoside might be one of the toxic components of fraction 5. Chlorogenic acid [26], martynoside [25], acteoside and isoacteoside [27], baicalin [28], 20(S)-ginsenoside Rg 2 [29] were reported to have anti-oxidative activity in other cell models. The pre-protective activity against H 2 O 2 injury of jionoside A1 and jionoside B1 in vitro were reported the first time.…”

Section: Neuroprotective Effects Of Fes Against H 2 O 2 Induced Injurmentioning

confidence: 99%

Identification of bioactive ingredients with immuno-enhancement and anti-oxidative effects from Fufang-Ejiao-Syrup by LC–MS n combined with bioassays

Shen

Chen

Zhu

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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Baicalin protects PC-12 cells from oxidative stress induced by hydrogen peroxide via anti-apoptotic effects

Abstract: The results point to the possibility of the neuroprotective effects of baicalin on neuronal apoptosis induced by oxidative stress and indicate that activation of the JAK/STAT signalling pathway might involve the anti-apoptotic effect of baicalin.

Cited by 61 publications

References 25 publications

Dose-dependent protective effect of baicalin against testicular torsion-detorsion in rats

Dose-dependent protective effect of baicalin against testicular torsion-detorsion in rats

Propofol Protects Against H2O2-Induced Oxidative Injury in Differentiated PC12 Cells via Inhibition of Ca2+-Dependent NADPH Oxidase

Identification of bioactive ingredients with immuno-enhancement and anti-oxidative effects from Fufang-Ejiao-Syrup by LC–MS n combined with bioassays

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