Baicalin upregulates the genetic expression of antioxidant enzymes in Type-2 diabetic Goto-Kakizaki rats

Waisundara, Viduranga Y.; Siu, Sing Yung; Hsu, Annie; Huang, Dejian; Tan, B. T. G.

doi:10.1016/j.lfs.2011.03.009

Cited by 43 publications

(34 citation statements)

References 45 publications

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“…The extract of S. baicalensis has been reported to possess a variety of biological effects including anti-oxidant, anti-inflammatory, anti-bacterial, anti-tumor, and anti-diabetic activities [4][5][6][7][8]. Baicalin was shown to possess anti-diabetic activities in type 2 diabetic Goto-Kakizaki rats [9], and baicalin, baicalein, and wogonin were found to be α-glucosidase inhibitors, which are considered to be potential leading compounds for development of anti-diabetes drug [10]. It is well documented that the incidence rate of type 2 diabetes is escalating worldwide partly due to the increasing prevalence of obesity [11], suggesting a potential relation between anti-obesity and anti-diabetic activity.…”

Section: Introductionmentioning

confidence: 99%

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

et al. 2016

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Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was used as solid phase extract absorbent for screening of lipase inhibitors from this plant. Three flavonoids were found to bind to LMNPs and were identified as baicalin, wogonin, and oroxylin A by liquid chromatography-mass spectrometry (HPLC-MS). Their IC 50 values were determined to be 229.22 ± 12.67, 153.71 ± 9.21, and 56.07 ± 4.90 μM, respectively. Fluorescence spectroscopy and molecular docking were used to probe the interactions between these flavonoids and lipase. All the flavonoids quenched the fluorescence of lipase statically by forming new complexes, implying their affinities with the enzyme. The thermodynamic analysis suggested that van der Waals force and hydrogen bond were the main forces between wogonin and lipase, while hydrophobic force was the main force for the other two flavonoids. The results from a molecular docking study further revealed that all of them could insert into the pocket of lipase binding to a couple of amino acid residues.

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Section: Introductionmentioning

confidence: 99%

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

et al. 2016

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“…1) in the traditional Chinese medicinal herb 'Huang qin' (Scutellaria baicalensis Georgi) (24,25). The baicalin exhibits many different pharmacological actions such as anti-oxidant (26), photo-protective (27), neural protective (28,29), antidepressant (30), anti-inflammatory (31,32), anti-viral (33,34), anti-hepatotoxicity (35,36) and anticancer effects (37)(38)(39). Baicalin induces CA46 Burkitt lymphoma cell apoptosis through inhibiting the PI3K/Akt kinase activity (40).…”

Section: Introductionmentioning

confidence: 99%

AKT serine/threonine protein kinase modulates baicalin-triggered autophagy in human bladder cancer T24 cells

Lin

Tsai

Tseng

et al. 2013

International Journal of Oncology

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Baicalin is one of the major compounds in the traditional Chinese medicinal herb from Scutellaria baicalensis Georgi. We investigated the molecular mechanisms of cell autophagy induced by baicalin in human bladder cancer T24 cells. Baicalin inhibited cell survival as shown by MTT assay and increased cell death by trypan blue exclusion assay in a concentration-dependent manner. Baicalin did not induce apoptotic cell death in T24 cells by TUNEL and caspase-3 activity assay. Baicalin induced the acidic vesicular organelle cell autophagy marker, manifested by acridine orange (AO) and monodansylcadaverine (MDC) staining and cleavage of microtubule-associated protein 1 light chain 3 (LC3). The protein expression levels of the Atg 5, Atg 7, Atg 12, Beclin-1 and LC3-II were upregulated in T24 cells after baicalin treatment. Inhibition of autophagy by 3-methyl-adenine (an inhibitor of class III phosphatidylinositol-3 kinase; 3-MA) reduced the cleavage of LC3 in T24 cells after baicalin treatment. Furthermore, protein expression levels of phospho-AKT (Ser473) and enzyme activity of AKT were downregulated in T24 cells after baicalin treatment. In conclusion, baicalin triggered cell autophagy through the AKT signaling pathway in T24 cells.

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“…1 A) as its major bioactive component [1]. Baicalin has wideranging pharmacological effects including anti-inflammatory [2], antiviral [3], anxiolytic [4], and antioxidant [5] activities. Moreover, the beneficial effect of baicalin on liver injury has also been demonstrated recently [6,7].…”

Section: Introductionmentioning

confidence: 99%

Ursodeoxycholic Acid Pretreatment Reduces Oral Bioavailability of the Multiple Drug Resistance-Associated Protein 2 Substrate Baicalin in Rats

Yan

et al. 2013

Planta Med

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Baicalin is a major bioactive component of Scutellaria baicalensis and a substrate of multiple drug resistance-associated protein 2. Expression of multiple drug resistance-associated protein 2 is regulated by NF-E2-related factor 2. The aim of this study was to explore whether ursodeoxycholic acid, an NF-E2-related factor 2 activator, could influence the oral bioavailability of baicalin. A single dose of baicalin (200 mg/kg) was given orally to rats pretreated with ursodeoxycholic acid (75 mg/kg and 150 mg/kg, per day, intragastrically) or normal saline (per day, intragastrically) for six consecutive days. The plasma concentration of baicalin was measured with the HPLC method. The result indicated that the oral bioavailability of baicalin was significantly and dose-dependently reduced in rats pretreated with ursodeoxycholic acid. Compared with control rats, the mean area under concentration-time curve of baicalin was reduced from 13.25 ± 0.24 mg/L h to 7.62 ± 0.15 mg/L h and 4.97 ± 0.21 mg/L h, and the C(max) value was decreased from 1.31 ± 0.03 mg/L to 0.62 ± 0.05 mg/L and 0.36 ± 0.04 mg/L in rats pretreated with ursodeoxycholic acid at doses of 75 mg/kg and 150 mg/kg, respectively, for six consecutive days. Hence, ursodeoxycholic acid treatment reduced the oral bioavailability of baicalin in rats, probably due to the enhanced efflux of baicalin from the intestine and liver by multiple drug resistance-associated protein 2.

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Baicalin upregulates the genetic expression of antioxidant enzymes in Type-2 diabetic Goto-Kakizaki rats

Cited by 43 publications

References 45 publications

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

AKT serine/threonine protein kinase modulates baicalin-triggered autophagy in human bladder cancer T24 cells

Ursodeoxycholic Acid Pretreatment Reduces Oral Bioavailability of the Multiple Drug Resistance-Associated Protein 2 Substrate Baicalin in Rats

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