Balancing pH and Yield: Exploring Itaconic Acid Production in Ustilago cynodontis from an Economic Perspective

Abstract

Background: Itaconic acid is a promising bio-based building block for the synthesis of polymers, plastics, fibers and other materials. In recent years, Ustilago cynodontis has emerged as an additional itaconate producing non-conventional yeast, mainly due to its high acid tolerance, which significantly reduces saline waste coproduction during fermentation and downstream processing. As a result, this could likely improve the economic viability of the itaconic acid production process with Ustilaginaceae. Results: In this study, we characterized a previously engineered itaconate hyper-producing Ustilago cynodontis strain in controlled fed-batch fermentations to determine the minimal and optimal pH for itaconate production. Under optimal fermentation conditions, the hyper-producing strain can achieve the theoretical maximal itaconate yield during the production phase in a low-density fermentation at pH 3.6, but at the expense of considerable base addition. Base consumption is strongly reduced at the pH of 2.8, but at cost of production yield, titer, and rate. A techno-economic analysis based on the entire process demonstrated that savings due to an additional decrease in pH control reagents and saline waste costs cannot compensate the yield loss observed at the highly acidic pH value 2.8. Conclusions: Overall, this work provides novel data regarding the individual strain properties and production capabilities, contributing to a better understanding of the itaconic acid production process with Ustilago cynodontis, especially from an economic perspective.

DoE-based medium optimization for improved biosurfactant production with Aureobasidium pullulans

“…The wide pH range reflects the robustness of A. pullulans and can help avoid the need for sterilization. Furthermore, extreme pH values reduce the risk of contamination during continuous operations and enables semi-sterile feeding of industrial feedstocks ( Li et al, 2014 ; Ernst et al, 2024 ). Besides advantages in energy consumption, the pH value can also positively affect production.…”