Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli

Deng, Yu; Ma, Ning; Zhu, Kangjia; Mao, Yin; Wei, Xuetuan; Zhao, Yang

doi:10.1016/j.ymben.2018.02.008

Cited by 58 publications

(53 citation statements)

References 17 publications

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“…Using the natural pathway, no <13 genetic modifications were executed to achieve a production of 52 g/L glycolate from glucose at a yield of 50 % of the Y st (Soucaille, 2007). Further genetic modifications were proposed by Deng et al (2018), by combining Dahms pathway with the glyoxylate shunt, enabling a yield close to 90% of the stoichiometric yield. The problem to reach the thermodynamic Y E lies at two levels.…”

Section: The Glycoptimus Pathway To Convert Sugars Into Glycolic Acidmentioning

confidence: 99%

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

François

Lachaux²,

Morin

2020

Front. Bioeng. Biotechnol.

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The global warming conjugated with our reliance to petrol derived processes and products have raised strong concern about the future of our planet, asking urgently to find sustainable substitute solutions to decrease this reliance and annihilate this climate change mainly due to excess of CO 2 emission. In this regard, the exploitation of microorganisms as microbial cell factories able to convert non-edible but renewable carbon sources into biofuels and commodity chemicals appears as an attractive solution. However, there is still a long way to go to make this solution economically viable and to introduce the use of microorganisms as one of the motor of the forthcoming bio-based economy. In this review, we address a scientific issue that must be challenged in order to improve the value of microbial organisms as cell factories. This issue is related to the capability of microbial systems to optimize carbon conservation during their metabolic processes. This initiative, which can be addressed nowadays using the advances in Synthetic Biology, should lead to an increase in products yield per carbon assimilated which is a key performance indice in biotechnological processes, as well as to indirectly contribute to a reduction of CO 2 emission.

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Section: The Glycoptimus Pathway To Convert Sugars Into Glycolic Acidmentioning

confidence: 99%

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

François

Lachaux²,

Morin

2020

Front. Bioeng. Biotechnol.

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“…However, in bioreactor, low biomass and sensitivity of cells to GA and high concentrations of D-glucose caused problems making it necessary carefully control carbon/nitrogen ratio and D-glucose feeding. However, so far, the highest GA production titers from GS were reported (Table 1 ) (65.5 g/L, 90% of the theoretical yield) (Deng et al 2018 ).…”

Section: Synthetic Pathways For Glycolic Acid and Ethylene Glycol Promentioning

confidence: 99%

“…However, in yeast, this may be hindered by the mitochondrial localisation of these enzymes while GS reactions take place in cytosol. Deng et al 2018 reported recently GA production of 90% of the theoretical yield with E. coli strain with modifications addressing the most of the aforementioned obstacles of GS. However, the GA titer 65 g/L and rate 0.85 g/L/h need to be improved for viable biotechnological process.…”

Section: Theoretical Comparison Of Different Routes For Glycolic Acidmentioning

confidence: 99%

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Biotechnological production of glycolic acid and ethylene glycol: current state and perspectives

Salusjärvi

Havukainen

Koivistoinen

et al. 2019

Appl Microbiol Biotechnol

108

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Glycolic acid (GA) and ethylene glycol (EG) are versatile two-carbon organic chemicals used in multiple daily applications. GA and EG are currently produced by chemical synthesis, but their biotechnological production from renewable resources has received a substantial interest. Several different metabolic pathways by using genetically modified microorganisms, such as Escherichia coli , Corynebacterium glutamicum and yeast have been established for their production. As a result, the yield of GA and EG produced from sugars has been significantly improved. Here, we describe the recent advancement in metabolic engineering efforts focusing on metabolic pathways and engineering strategies used for GA and EG production.

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“…1e). Afterwards, the pyruvate was transported into the cell by its carrier protein [30,31] for the tricarboxylic acid (TCA) cycle [32]. Together, in this round of lactose intake, the engineered bacteria finished the lactose digestion as well as pH rescue.…”

Section: The Tri-stable-switch Circuit Can Switch Between Two Functiomentioning

confidence: 99%

An Engineered Genetic Circuit for Lactose Intolerance Alleviation Coupled with Gut Microbiota Recovery

Cheng

et al. 2020

Preprint

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BackgroundLactose malabsorption occurs in around 68% of the world populations, causing lactose intolerance (LI) symptoms such as abdominal pain, bloating and diarrhoea. To alleviate LI, previous studies mainly focused on strengthening intestinal β-galactosidase activity but neglected the inconspicuous colon pH drop caused by gut microbes’ fermentation on non-hydrolysed lactose. The colon pH drop will reduce intestinal β-galactosidase activity and influence the intestinal homeostasis.ResultsHere, we synthesized a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability. This circuit can switch in functionality between expression of β-galactosidase and expression of l-lactate dehydrogenase in respond to intestinal lactose signal and intestinal pH signal. We confirmed the circuit functionality was efficient using 12-hrs in vitro culture at a range of pH levels, as well as 6-hrs in vivo simulations in mice colon. Moreover, another 21-days mice trial indicated that this circuit can recover lactose-effected gut microbiota of mice to the status (enterotypes) similar to that of mice without lactose intake.ConclusionsTaken together, the tri-stable-switch circuit can serve as a promising prototype for LI symptoms relief, especially by flexibly adapting to environmental variation, stabilizing colon pH and restoring gut microbiota.

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Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli

Cited by 58 publications

References 17 publications

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

Synthetic Biology Applied to Carbon Conservative and Carbon Dioxide Recycling Pathways

Biotechnological production of glycolic acid and ethylene glycol: current state and perspectives

An Engineered Genetic Circuit for Lactose Intolerance Alleviation Coupled with Gut Microbiota Recovery

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