BAMBI promotes macrophage proliferation and differentiation in gliomas

Wang, Dan; Chen, Xiaolai; Zhang, Ran

doi:10.3892/mmr.2017.8320

Cited by 7 publications

(6 citation statements)

References 29 publications

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“…In order to validate whether MAN2B1 expression may influence the macrophage subtypes in glioma, we analyzed the correlation between MAN2B1 and TAMs biomarker genes. As expected, we found that M1 macrophage marker genes, such as NOS2 and TNF, were weakly correlated with MAN2B1 expression (45,46), whereas M2 macrophage marker genes CD163 and TGFBI were strongly correlated (47,48). Moreover, TAMs marker genes, including CCL2 and IL10, had moderate correlations with MAN2B1 (49,50).…”

Section: Discussionsupporting

confidence: 81%

Immune Infiltration Associated MAN2B1 Is a Novel Prognostic Biomarker for Glioma

et al. 2022

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Mannosidase Alpha Class 2B Member 1 (MAN2B1) gene encodes lysosomal alpha-d-mannosidase involved in the ordered degradation of N-linked glycoproteins. Alteration in MAN2B1 has been proved to be accountable for several diseases. However, the relationship between MAN2B1 and glioma malignancy remains unclear. In this study, RNA-seq data from The Cancer Genome Atlas and the Chinese Glioma Genome Atlas datasets were analyzed to explore the correlation between MAN2B1 and clinicopathological features, prognosis, and somatic mutations in gliomas. We found that MAN2B1 was elevated in glioma and was correlated with malignant clinical and molecular features. Upregulated expression of MAN2B1 is prognostic for poor outcomes in glioma patients. Different frequencies of somatic mutations were found in gliomas between high and low MAN2B1 expression. Real-time quantitative polymerase chain reaction, western blot, and immunohistochemistry staining from glioma patient samples and cell lines were used to validate bioinformatic findings. Functional enrichment analysis showed that MAN2B1 was involved in immune and inflammation processes. Moreover, MAN2B1 expression was strongly correlated with M2 macrophages and weakly correlated with M1 macrophages. Further analysis confirmed that MAN2B1 was closely associated with the markers of M2 macrophages and tumor-associated macrophages. Taken together, MAN2B1 is a potential prognostic biomarker in glioma and associates with immune infiltration.

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Section: Discussionsupporting

confidence: 81%

Immune Infiltration Associated MAN2B1 Is a Novel Prognostic Biomarker for Glioma

et al. 2022

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“…WDR1 took part in inducing the disassembly of actin filaments, and dysfunction of WDR1 might cause autoinflammatory disease, which in turn activated macrophage [ 43 ]. BAMBI, a pseudoreceptor of the TGF signaling pathway, played a key role in regulating macrophage polarization [ 44 ]. A former study indicated that highly expressed BAMBI could contribute to colon cancer metastasis through Wnt/beta-catenin in mice models [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Construction and Validation of a Macrophage-Associated Risk Model for Predicting the Prognosis of Osteosarcoma

Xiao

Liu

Zeng

et al. 2021

Journal of Oncology

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Background. Osteosarcoma is one of the most common bone tumors among children. Tumor-associated macrophages have been found to interact with tumor cells, secreting a variety of cytokines about tumor growth, metastasis, and prognosis. This study aimed to identify macrophage-associated genes (MAGs) signatures to predict the prognosis of osteosarcoma. Methods. Totally 384 MAGs were collected from GSEA software C7: immunologic signature gene sets. Differential gene expression (DGE) analysis was performed between normal bone samples and osteosarcoma samples in GSE99671. Kaplan–Meier survival analysis was performed to identify prognostic MAGs in TARGET-OS. Decision curve analysis (DCA), nomogram, receiver operating characteristic (ROC), and survival curve analysis were further used to assess our risk model. All genes from TARGET-OS were used for gene set enrichment analysis (GSEA). Immune infiltration of osteosarcoma sample was calculated using CIBERSORT and ESTIMATE packages. The independent test data set GSE21257 from gene expression omnibus (GEO) was used to validate our risk model. Results. 5 MAGs (MAP3K5, PML, WDR1, BAMBI, and GNPDA2) were screened based on protein-protein interaction (PPI), DGE, and survival analysis. A novel macrophage-associated risk model was constructed to predict a risk score based on multivariate Cox regression analysis. The high-risk group showed a worse prognosis of osteosarcoma ( p < 0.001) while the low-risk group had higher immune and stromal scores. The risk score was identified as an independent prognostic factor for osteosarcoma. MAGs model for diagnosis of osteosarcoma had a better net clinical benefit based on DCA. The nomogram and ROC curve also effectively predicted the prognosis of osteosarcoma. Besides, the validation result was consistent with the result of TARGET-OS. Conclusions. A novel macrophage-associated risk score to differentiate low- and high-risk groups of osteosarcoma was constructed based on integrative bioinformatics analysis. Macrophages might affect the prognosis of osteosarcoma through macrophage differentiation pathways and bring novel sights for the progression and prognosis of osteosarcoma.

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“…10 Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) is a transmembrane glycoprotein and upregulated in gastric cancer, 11 gliomas. 12 However, the mechanisms of miR-17-5p and BAMBI have scarcely reported in NSCLC. In this study, we mainly explored the effects of PVT1 in NSCLC.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Non-Coding RNA PVT1 Regulates BAMBI To Promote Tumor Progression In Non-Small Cell Lung Cancer By Sponging miR-17-5p</p>

Wang¹,

Zhang²,

Sun³

et al. 2020

OTT

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Background: Non-small cell lung cancer (NSCLC) is a common malignancy over the world. Previous report indicated that the plasmacytoma variant translocation 1 (PVT1) has been documented to function as an oncogene in various types of human cancers. However, the biological mechanism of PVT1 was still rarely reported in NSCLC. Methods: The levels of PVT1, miR-17-5p, and bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) in NSCLC tissues (n=64) and cells (H1299 and A549) were detected by qRT-PCR and immunohistochemistry (IHC). The protein level of BAMBI was measured by Western blot assay. Cell viability and apoptotic rate were evaluated by MTT assay and flow cytometry, respectively. The migrated and invaded abilities were assessed by Transwell assay and Wound healing assay. The interactions between miR-17-5p and PVT1 or BAMBI were predicted by starBase v2.0 and TargetScan, respectively, and then dual-luciferase reporter assay and RNA pull-down assay were performed to verify these interactions. The mice model experiments were constructed to further validate the roles of PVT1 in vivo. Results: The levels of PVT1 and BAMBI were both apparently increased, and miR-17-5p was declined in NSCLC tissues and cells. The depletion of PVT1 or BAMBI blocked cell viability, migrated and invaded abilities but impelled apoptotic rate in A549 and H1299 cells. PVT1 was validated as a sponge to miR-17-5p and BAMBI was a direct target of miR-17-5p. PVT1 promoted cell viability, migrated and invaded abilities but repressed apoptotic rate by targeting BAMBI. MiR-17-5p regulated cell behaviors mediated by PVT1. PVT1 silencing decreased BAMBI expression by sponging miR-17-5p. In addition, PVT1 knockdown blocked the xenograft tumor growth in vivo. Conclusion: These results manifested that PVT1 modulated BAMBI to promote tumor progression in NSCLC by sponging miR-17-5p. Thus, the novel regulatory pathway may provide a new therapeutic target for NSCLC patients.

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BAMBI promotes macrophage proliferation and differentiation in gliomas

Cited by 7 publications

References 29 publications

Immune Infiltration Associated MAN2B1 Is a Novel Prognostic Biomarker for Glioma

Immune Infiltration Associated MAN2B1 Is a Novel Prognostic Biomarker for Glioma

Construction and Validation of a Macrophage-Associated Risk Model for Predicting the Prognosis of Osteosarcoma

<p>Long Non-Coding RNA PVT1 Regulates BAMBI To Promote Tumor Progression In Non-Small Cell Lung Cancer By Sponging miR-17-5p</p>

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