Bamboo shoot dietary fiber alleviates gut microbiota dysbiosis and modulates liver fatty acid metabolism in mice with high-fat diet-induced obesity

Abstract

IntroductionObesity is a common nutritional disorder characterized by an excessive fat accumulation. In view of the critical role of gut microbiota in the development of obesity and metabolic diseases, novel dietary therapies have been developed to manage obesity by targeting the gut microbiome. In this study, we investigated anti-obesity effects of bamboo shoot dietary fiber (BSDF) and the potential mechanisms.MethodsAfter 12 weeks of intervention with BSDF in high-fat mice, we detected obesity-related phenotypic indicators, and made transcriptomic analysis of liver tissue. Then we analyzed the changes of gut microbiota using 16S rRNA gene sequencing, explored the effect of BSDF on gut microbiota metabolites, and finally verified the importance of gut microbiota through antibiotic animal model.Results and discussionWe found that BSDF was effective in reducing lipid accumulation in liver and adipose tissue and alleviating dyslipidemia and insulin resistance. Liver transcriptome analysis results showed that BSDF could improve lipid metabolism and liver injury by modulating peroxisome proliferator-activated receptor (PPAR) and fatty acid metabolic pathways. The 16S rRNA gene sequencing analysis of gut microbiota composition showed that BSDF significantly enriched beneficial bacteria such as Bifidobacterium, Akkermansia, Dubosiella, and Alloprevotella. Analysis of fecal metabolomics and gut microbiota metabolites revealed that BSDF increased the levels of several short-chain fatty acids and enriched bile acids, which may be important for improving lipid metabolism. Notably, the obesity-related metabolic disorders were abrogated after the abrogation of gut microbiota, suggesting that gut microbiota is a key factor in the beneficial effects of BSDF.ConclusionOur study suggests that BSDF as a prebiotic supplement has the potential to improve obesity by improving gut microbiota and modulating host PPAR and fatty acid metabolic pathways.

Blueberry and Blackberry Anthocyanins Ameliorate Metabolic Syndrome by Modulating Gut Microbiota and Short-Chain Fatty Acids Metabolism in High-Fat Diet-Fed C57BL/6J Mice

“…57,58 Generally, the intervention of HFD can disrupt the balance of the gut microbiota, especially by increasing the abundance of Firmicutes, decreasing the abundance of Bacteroidetes, and elevating the ratio of F/B, which was significantly associated with obesity and diabetes. 59 In our study, it was discovered that Bacteroidetes and Firmicutes were the dominant phyla in the four groups, similar to previous studies. 60 Furthermore, significant variations in the relative abundance of Bacteroidetes and Firmicutes were detected between the HFD and Chow groups.…”

Evaluation of Prebiotic Activity of Stellariae Radix Polysaccharides and Its Effects on Gut Microbiota

“…Additionally, Dubosiella can increase the abundance of beneficial microorganisms (Bifidobacterium and Lactobacillus) in obese mice, improve the disorder of intestinal flora structure caused by a high-fat diet, improve intestinal metabolism and immunity, and reduce the occurrence of bacterial infections and other diseases. Numerous studies have also found that the content of Dubosiella increases during the treatment of obesity [26][27][28][29][30][31][32]. It has been proven to improve the metabolism of the body, normalize blood lipid metabolism, and increase the content of short-chain fatty acids (SCFAs) such as butyrate.…”

The Role of Dietary Fibre in Enteral Nutrition in Sepsis Prevention and Therapy: A Narrative Review

“…Other papers support the fact that the effect of DFs highly depends on the type of DF by showing that inulin DF diets can trigger eosinophilia and Th2 immune cell response not only in the intestine but also in the lungs, which in excess are hallmarks for allergic asthma [123]. Further, Zhou and colleagues showed that some insoluble DFs such as bamboo shoot DF increased SCFA and BA levels influencing lipid metabolism in mice fed a high-fat diet [124]. Furthermore, a low-DF westernised diet was associated with BA profile dysregulations contributing to the development of chronic inflammatory diseases such as diabetes type 2 and colon cancer, and this might be alleviated by DF supplementation since soluble DFs increase the SCFA levels [50,125,126].…”