Banana Ripening: Implications of Changes in Glycolytic Intermediate Concentrations, Glycolytic and Gluconeogenic Carbon Flux, and Fructose 2,6-Bisphosphate Concentration

Beaudry, Randolph M.; Severson, Ray F.; Black, Clanton C.; Kays, Stanley J.

doi:10.1104/pp.91.4.1436

Cited by 84 publications

(85 citation statements)

References 25 publications

(39 reference statements)

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“…The response of the rin, behaving like nonclimacteric fruit (8), is also significant; although in this tissue, the magnitude of the 14C recovery in the sugar fraction is equivalent to that of the green Rutgers fruit, the AA-treated rin mimicked a metabolic activity found in climacteric fruit. In ripening banana, starch is degraded to triose or hexose phosphates which diffuse from the amyloplast to the cytosol and are metabolically apportioned for glycolysis and in addition for gluconeogenesis (1). The present work is an additional example of ripening-related gluconeogenesis suggesting that the process may be common in ripening fruit.…”

Section: Resultsmentioning

confidence: 70%

“…In AA-held fruit there was in addition further and substantial diminution in the Fru 2,6-P2 concentration amounting to one-half to one-third of the level found in air-held fruit. In mature banana fruit, (1). Figure 6 shows that in the tomato pericarp tissue the AA-induced reduction in Fru 2,6-P2 by similar ratios is associated with a comparable change, namely, stimulation of gluconeogenesis-driven carbon movement (Fig.…”

Section: Resultsmentioning

confidence: 89%

“…The composition of the sugar fraction (b) was verified by converting the sugars to their trimethyl silyl derivatives and analysis by gas chromatography as outlined previously (21) and essentially as described by Beaudry et al (1). This fraction was found to contain fructose and glucose as major components in addition to sucrose (Fig.…”

mentioning

confidence: 94%

“…In banana, gluconeogenesis from starch degradation products was shown to be stimulated by ethylene (1). By comparison, AA stimulation ofsugar accumulation is not restricted to fruit with pronounced ethylene synthesis and action (13,19) (19).…”

mentioning

confidence: 96%

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Acetaldehyde Stimulation of Net Gluconeogenic Carbon Movement from Applied Malic Acid in Tomato Fruit Pericarp Tissue

Halinska¹,

Frenkél²

1991

Plant Physiol.

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Applied acetaldehyde is known to lead to sugar accumulation in fruit including tomatoes (Lycopersicon esculentum) (O Paz, HW Janes, BA Prevost, C Frenkel [1982] presumably due to stimulation of gluconeogenesis. This conjecture was examined using tomato fruit pencarp discs as a test system and applied -[U-14C]malic acid as the source for gluconeogenic carbon mobilization. The label from malate was recovered in respiratory C02, in other organic acids, in ethanol insoluble material, and an appreciable amount in the ethanol soluble sugar fraction. In Rutgers tomatoes, the label recovery in the sugar fraction and an attendant label reduction in the organic acids fraction intensified with fruit ripening. In both Rutgers and in the nonripening tomato rin, these processes were markedly stimulated by 4000 ppm acetaldehyde. The onset of label apportioning from malic acids to sugars coincided with decreased levels of fructose-2,6-biphosphate, the gluconeogenesis inhibitor.In acetaldehyde-treated tissues, with enhanced label mobilization, this decline reached one-half to one third of the initial fructose-2,6-biphosphate levels. Application of 30 micromolar fructose-2,6-biphosphate or 2,5-anhydro-d-mannitol in tum led to a precipitous reduction in the label flow to sugars presumably due to inhibition of fructose-1,6-biphosphatase by the compounds. We conclude that malic and perhaps other organic acids are carbon sources for gluconeogenesis occurring normally in ripening tomatoes. The process is stimulated by acetaldehyde apparently by attenuating the fructose-2,6-biphosphate levels. The mode of the acetaldehyde regulation of fructose-2,6-biphosphate metabolism awaits clarification.AA4 is a common volatile in plants that accumulate during physiological disorders (25) and in ripening fruit (7,14).

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Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 89%

mentioning

confidence: 94%

mentioning

confidence: 96%

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Acetaldehyde Stimulation of Net Gluconeogenic Carbon Movement from Applied Malic Acid in Tomato Fruit Pericarp Tissue

Halinska¹,

Frenkél²

1991

Plant Physiol.

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“…Stimulation of carbon flux into the TCA cycle through activation of PK is traditionally depicted by a decrease in PEP and an attendant increase in pyruvate levels (Kobr and Beevers, 1971;Turner and Turner, 1980;Beaudry et al, 1989). In sugarcane, the ratio of pyruvate to PEP remained unchanged, indicating that there is no change in the regulation of PK activity associated with the decrease in respiration.…”

Section: Regulation Of Carbon Flowmentioning

confidence: 99%

Carbon Partitioning during Sucrose Accumulation in Sugarcane Internodal Tissue

1997

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l h e temporal relationship between sucrose (Suc) accumulation and carbon partitioning was investigated in developing sugarcane internodes. Radiolabeling studies on tissue slices, which contained Suc concentrations ranging from 14 to 42% of the dry mass, indicated that maturation coincided with a redirection of carbon from water-insoluble matter, respiration, amino acids, organic acids, and phosphorylated intermediates into SUC. It is evident that a cycle of Suc synthesis and degradation exists in all of the internodes. l h e decreased allocation of carbon to respiration coincides with a decreased flux from the hexose pool. 60th the glucose and fructose (Fru) concentrations significantly decrease during maturation. The phosphoenolpyruvate, Fru-6-phosphate (Fru-6-P), and Fru-2,6-bisphosphate (Fru-2, 6-P,) concentrations decrease between the young and older internodal tissue, whereas the inorganic phosphate concentration increases. lhe calculated mass-action ratios indicate that the ATP-dependent phosphofructokinase, pyruvate kinase, and Fru-l,6-bisphosphatase reactions are tightly regulated in all of the internodes, and no evidence was found that major changes in the regulation of any of these enzymes occur. l h e pyrophosphatedependent phosphofructokinase reaction is in apparent equilibrium in ali the internodes. Substrate availability might be one of the prime factors contributing to the observed decrease in respiration.

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Biochemistry of Fruits

Paliyath

Murr²

2006

Food Biochemistry and Food Processing

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Banana Ripening: Implications of Changes in Glycolytic Intermediate Concentrations, Glycolytic and Gluconeogenic Carbon Flux, and Fructose 2,6-Bisphosphate Concentration

Cited by 84 publications

References 25 publications

Acetaldehyde Stimulation of Net Gluconeogenic Carbon Movement from Applied Malic Acid in Tomato Fruit Pericarp Tissue

Acetaldehyde Stimulation of Net Gluconeogenic Carbon Movement from Applied Malic Acid in Tomato Fruit Pericarp Tissue

Carbon Partitioning during Sucrose Accumulation in Sugarcane Internodal Tissue

Biochemistry of Fruits

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