Band composition of electrophoretic spectra of storage proteins in interspecific pea hybrids

Бобков, С В; Лазарева, Т. Н.

doi:10.1134/s1022795411110068

Cited by 13 publications

(5 citation statements)

References 8 publications

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“…), vitelline envelope (Aagaard et al . ), seeds storage proteins (Bobkov & Lazareva ), eyes tear film (Green‐Church et al . ), liver and brain extracts (Gonzalez et al .…”

Section: Discussionmentioning

confidence: 99%

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Statistical analysis of the individual variability of 1D protein profiles as a tool in ecology: an application to parasitoid venom

Mathe‐Hubert

Gatti

Colinet

et al. 2015

Molecular Ecology Resources

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Understanding the forces that shape eco-evolutionary patterns often requires linking phenotypes to genotypes, allowing characterization of these patterns at the molecular level. DNA-based markers are less informative in this aim compared to markers associated with gene expression and, more specifically, with protein quantities. The characterization of eco-evolutionary patterns also usually requires the analysis of large sample sizes to accurately estimate interindividual variability. However, the methods used to characterize and compare protein samples are generally expensive and time-consuming, which constrains the size of the produced data sets to few individuals. We present here a method that estimates the interindividual variability of protein quantities based on a global, semi-automatic analysis of 1D electrophoretic profiles, opening the way to rapid analysis and comparison of hundreds of individuals. The main original features of the method are the in silico normalization of sample protein quantities using pictures of electrophoresis gels at different staining levels, as well as a new method of analysis of electrophoretic profiles based on a median profile. We demonstrate that this method can accurately discriminate between species and between geographically distant or close populations, based on interindividual variation in venom protein profiles from three endoparasitoid wasps of two different genera (Psyttalia concolor, Psyttalia lounsburyi and Leptopilina boulardi). Finally, we discuss the experimental designs that would benefit from the use of this method.

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“…), vitelline envelope (Aagaard et al . ), seeds storage proteins (Bobkov & Lazareva ), eyes tear film (Green‐Church et al . ), liver and brain extracts (Gonzalez et al .…”

Section: Discussionmentioning

confidence: 99%

“…1D electrophoresis, a simple and low‐cost method, has rarely been used to estimate interindividual variability in eco‐evolutionary studies (see Bobkov & Lazareva ; Krishnan et al . ).…”

Section: Introductionmentioning

confidence: 99%

Statistical analysis of the individual variability of 1D protein profiles as a tool in ecology: an application to parasitoid venom

Mathe‐Hubert

Gatti

Colinet

et al. 2015

Molecular Ecology Resources

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“…В качестве стандарта использован электрофоретический спектр белков семян сои сорта 'Светлая' (к-9960). При описании компонентов электрофоретических спектров белков принимали во внимание данные С. В. Бобкова и Т. Н. Лазаревой, полученные при исследовании межвидовых гибридов гороха (Bobkov, Lazareva, 2012).…”

Section: материал и методыunclassified

Identification of duplicate accessions in the pea (Pisum sativum L.) collection at VIR

Семенова

Vasipov

Anisimova

2022

Trudy po prikladnoy botanike, genetike i selektsii

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Background. Identification of duplicates in the collections of genetic resources is the most important problem of seed gene bank management. Duplicate accessions expand the collection size, thus raising the costs of germplasm maintenance without broadening the genetic diversity.Materials and methods. The studied material included 17 pairs of Pisum sativum L. accessions from the VIR collection which presumably had been erroneously registered twice in the VIR catalogue; however, they had identical introductory numbers. The accessions entered the collection in 1922–1996 and to date they have been reproduced 2 to 16 times. After a field assessment, 15 pairs of putative duplicate accessions of various uses were selected for molecular analysis. A RAPD analysis was performed using five primers from the Operon Series. Total seed proteins were analyzed by SDS-polyacrylamide gel electrophoresis.Results. The following criteria were used to ascertain identity of the accessions or their difference: 1) similarity of morphological characters (habitus, and anthocyanin pigmentation of flowers and vegetative organs) and flowering dates; 2) identity or polymorphism of RAPD profiles; and 3) identity or difference in electrophoretic banding patterns of seed storage proteins. Seven pairs of duplicates were identified according to the results of a comparative analysis. Among them, the accessions in the pairs k-81/k-1199, k-8331/k-8645, k-8719/k-8760, and k-8757/k-8825 turned out to be completely identical, while k8464/k-8472, k-8740/k-8873, and k-8689/k-8723 were heterogenic, but had similar RAPD profiles and seed proteins patterns.Conclusions. An integrated assessment involving in-field plant phenotyping and analyzing polymorphism of amplified DNA fragments and components in electrophoretic banding patterns of seed proteins is promising for detecting identical or heterogenic accessions in genebank collections.

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“…На территории России ядрица гречихи является лидером среди всех вырабатываемых в нашей стране круп на протяжении последних 10 лет [4]. Глубокая переработка зерна гречихи используется для получения белковых изолятов, резистентного крахмала и биофлавоноида рутина [1,30]. Для увеличения продолжительности хранения гречневую крупу подвергают термической обработке, однако биохимический состав термически обработанной гречневой крупы уступает непропаренному варианту, который получают путём последовательного шелушения крупы, не подвергая воздействию нагрева и пара [18].…”

Section: Introductionunclassified

Development of grain drink technology based on unsteamed buckwheat

Kerimova,

Fomenko,

Pivchenko

et al. 2023

Novye tehnologii

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Herbal drinks are products that have already become familiar to supporters of a «dairyfree » diet, vegetarians and people with lactase deficiency. There is a wide range of drinks on the market, among them the most popular types are cereals, legumes, nuts. It is difficult to find a worthy replacement for cow milk due to the specific organoleptic indicators of raw materials, the content of certain components that cause allergies or intolerance when they are used. As a substrate for the research, green buckwheat has been chosen, which does not contain gluten, has a rich biochemical composition and is a hypoallergenic product. Unlike heat-treated cereals, unsteamed cereals retain more functional components in their composition. The article proposes a method for obtaining a lactose-free and glutenfree vegetable drink based on unsteamed buckwheat. The aim of the research is to select the parameters for the production of a grain drink. The rational concentration of the introduced substrate and the dosage of enzyme preparations of maltogenic amylase and α-amylase have been determined. Taking into account the features characteristic of the hydrolysable raw materials, the parameters of enzymatic hydrolysis with a minimum temperature effect and holding time have been proposed. In the research amylolytic enzymes from Novozymes (Denmark) and Sibbiopharm (Russia) have been used to reduce the viscosity of a highstarch substrate, which complicates the technological process of producing a vegetable drink, and to create the desired organoleptic indicators. In the finished drink, the final indicators of the content of protein, fat, reducing substances, dry substances have been detected. The finished product is also a source of soluble dietary fiber, which has a positive effect on the human microbiome.

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Band composition of electrophoretic spectra of storage proteins in interspecific pea hybrids

Cited by 13 publications

References 8 publications

Statistical analysis of the individual variability of 1D protein profiles as a tool in ecology: an application to parasitoid venom

Statistical analysis of the individual variability of 1D protein profiles as a tool in ecology: an application to parasitoid venom

Identification of duplicate accessions in the pea (Pisum sativum L.) collection at VIR

Development of grain drink technology based on unsteamed buckwheat

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