Band Keratopathy in MRL/1 and MRL/n Mice

Hoffman, Robert W.; Yang, Hua; Waggie, Kim; Durham, J.; Burge, Johanna; Walker, Sara E.

doi:10.1002/art.1780260511

Cited by 27 publications

(8 citation statements)

References 14 publications

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“…In both humans and animals, band keratopathy has been associated with a variety of ocular and systemic conditions that, through a variety of mechanisms, leave Bowman’s membrane prone to calcification in areas of greatest environmental exposure. [ 24 , 25 ] In the context of anterior uveitis, band keratopathy is more frequently observed in children than adults. [ 26 , 27 ] Current hypotheses of pathophysiology center on events such as tear evaporation and changes in pH that may promote calcium precipitation, but much remains unknown concerning the precise mechanism(s).…”

Section: Discussionmentioning

confidence: 99%

Ketamine/Xylazine-Induced Corneal Damage in Mice

et al. 2015

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PurposeWe have observed that the commonly used ketamine/xylazine anesthesia mix can induce a focally severe and permanent corneal opacity. The purpose of this study was to establish the clinical and histological features of this deleterious side effect, its sensitivity with respect to age and anesthesia protocol, and approaches for avoiding it.MethodsYoung C57BL/6J, C57BLKS/J, and SJL/J mice were treated with permutations of anesthesia protocols and compared using slit-lamp exams, optical coherence tomography, histologic analyses, and telemetric measurements of body temperature.ResultsKetamine/xylazine induces corneal damage in mice with a variable frequency. Among 12 experimental cohorts, corneal damage associated with ketamine/xylazine was observed in 9 of them. Despite various treatments to avoid corneal dehydration during anesthesia, the frequency of corneas experiencing damage among responding cohorts was 42% (26% inclusive of all cohorts), which is significantly greater than the natural prevalence (5%). The damage was consistent with band keratopathy. It appeared as a white or gray horizontal band located proximal to the pupil and was positive for subepithelial calcium deposition with von Kossa stain.ConclusionsThe sum of our clinical and histological observations is consistent with ketamine/xylazine-induced band keratopathy in mice. This finding is relevant for mouse studies involving the eye and/or vision-dependent behavioral assays, which would both be prone to artifact without appreciation of the damage caused by ketamine/xylazine anesthesia. Use of yohimbine is suggested as a practical means of avoiding this complication.

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Section: Discussionmentioning

confidence: 99%

Ketamine/Xylazine-Induced Corneal Damage in Mice

et al. 2015

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“…Twenty to 100 serial lop sections were stained with hematoxylin and eosin. These tissue sections were in part the basis of an earlier study of band keratopathy and posterior uveitis in autoimmune and normal mice (13). The left lacrimal gland was removed separately and the parotid, submandibular, and sublingual salivary glands were taken en bloc.…”

Section: Methodsmentioning

confidence: 99%

Sjögren's syndrome in MRL/l and MRL/n mice

Hoffman

Alspaugh

Waggie

et al. 1984

Arthritis & Rheumatism

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139

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Six autoimmune murine models (MRLh, MRL/n, NZB, NZB/NZW, PN, C57BL/6J-lpr/lpr) were compared with normal control C57BL/6J and DBM2 mice to determine if spontaneous autoimmune disease was associated with evidence of Sjogren's syndrome. Schirmer tests documented dry eyes in NZB/NZW and PN mice; other autoimmune strains and controls had normal tear formation. All autoimmune mice had conjunctivitis, but this abnormality was most severe in the PN strain. Ninety-eight percent of MRL/I and MRL/n mice had mononuclear cell infiltrates in lacrimal glands, and salivary glands were involved to a lesser degree. New Zealand mice and PN mice had smaller gland lesions. The extensive gland destruction in MRL/I and MRLln mice suggested that these substrains merit further studies as animal models of Sjogren's syndrome. _ _ _ _From the Veterans Administration Medical Center, Columbia, Missouri and the University of MissouriXolumbia.

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“…It also follows localized damage or desiccation of the cornea. 299,300 Similar mineral deposits are reported to form in rats as a result of corneal desiccation which follows administration of morphine and in rabbits in association with local trauma and vitamin D excess. 294 Chelation of the calcium by use of ethylenediamine-tetraacetic acid (EDTA) is an effective remedy.…”

Section: Corneal Dystrophy: Mineralization Band Keratopathymentioning

confidence: 64%

Nervous System and Special Sense Organs

Greaves¹

2012

Histopathology of Preclinical Toxicity Studies

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Band Keratopathy in MRL/1 and MRL/n Mice

Cited by 27 publications

References 14 publications

Ketamine/Xylazine-Induced Corneal Damage in Mice

Ketamine/Xylazine-Induced Corneal Damage in Mice

Sjögren's syndrome in MRL/l and MRL/n mice

Nervous System and Special Sense Organs

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