Banding of Human Chromosomes by Protein Denaturation

Biophysical Studies on the Mechanism of Quinacrine Staining of Chromosomes

“…One of the most remarkable features of chromosome banding techniques is the similarity of bands produced by a variety of methods. Lee et al (1973) have identified four groups of techniques which produce similar patterns; these are (1) quinacrine fluorescence; (2) Giemsa in combination with alkali-heating techniques; (3) Giemsa in combination with any one of several proteolytic enzymes; and (4) Giemsa in combination with protein-denaturing substances such as 5 M urea and anionic and nonionic detergents. Until recently, it was thought that the Giemsa-alkali heating technique (number 2 above) reflected the differential chromosomal localization of classes of repetitious and nonrepetitious DNA.…”

Mechanism of chromosome banding: A phenomenological approach

“…If coefficients a and a' are almost negligible, then ea'(xn-xn-I)e-axn -1, and we have Eq. (19) as c,ck = a sin[p'(x, -x,-1)] cos(px,), (20) which yields a simple harmonic curve.…”

Mammalian chromosomes G-banded in four minutes

“…Sibertstein & Ewing (1972) described a technique which included a culture pretreatment, while Ansede (1972) required slides to be aged for 6-8 days prior to treatment. Lee et al (1973) described the use of detergents but the duration of treatment was longer than in the method described here, while Kato & Moriwaki (1972) required adjustment to pH of detergent.…”