Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina

Babu, Swathy; Takeuchi, Yuki; Masai, Ichiro

doi:10.7554/elife.74611

Cited by 9 publications

(7 citation statements)

References 98 publications

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“…Antibody concentrations used were 1:300 chicken anti-GFP (Abcam, ab13970, RRID: AB_300798), rabbit anti-RFP (Antibodies Online, ABIN129578, RRID: AB_10781500) or phospho-histone H3 (ref. 75) (pH3-ser10) mouse monoclonal antibody (Cell Signaling, #9706, RRID:AB_331748) primary antibodies and 1:400 Alexa Fluor 488 goat anti-chicken IgG165 (Thermo Fisher Scientific, cat. no.…”

Section: Immunofluorescencementioning

confidence: 99%

Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium

et al. 2023

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Definitive haematopoietic stem and progenitor cells (HSPCs) generate erythroid, lymphoid and myeloid lineages. HSPCs are produced in the embryo via transdifferentiation of haemogenic endothelial cells in the aorta–gonad–mesonephros (AGM). HSPCs in the AGM are heterogeneous in differentiation and proliferative output, but how these intrinsic differences are acquired remains unanswered. Here we discovered that loss of microRNA (miR)-128 in zebrafish leads to an expansion of HSPCs in the AGM with different cell cycle states and a skew towards erythroid and lymphoid progenitors. Manipulating miR-128 in differentiating haemogenic endothelial cells, before their transition to HSPCs, recapitulated the lineage skewing in both zebrafish and human pluripotent stem cells. miR-128 promotes Wnt and Notch signalling in the AGM via post-transcriptional repression of the Wnt inhibitor csnk1a1 and the Notch ligand jag1b. De-repression of cskn1a1 resulted in replicative and erythroid-biased HSPCs, whereas de-repression of jag1b resulted in G2/M and lymphoid-biased HSPCs with long-term consequence on the respective blood lineages. We propose that HSPC heterogeneity arises in the AGM endothelium and is programmed in part by Wnt and Notch signalling.

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Section: Immunofluorescencementioning

confidence: 99%

Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium

et al. 2023

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“…At the molecular level, BANP binds transcription factors at CpG islands and regulates metabolic genes in pluripotent stem cells [34]. In embryonic development, BANP is essential for the DNA damage response and chromosome segregation during mitosis [35]. In oncological diseases, BANP overexpression inhibits tumor progression [36,37].…”

Section: Discussionmentioning

confidence: 99%

BANP Participates in the Chronic Intermittent Hypoxia-Induced Senescence of Vascular Endothelial Cells by Promoting P53 Phosphorylation and Nuclear Retention

Li,

Zhao,

Liu

et al. 2024

Gerontology

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Introduction The objective of this study is to examine the potential induction of senescence in vascular endothelial cells (VECs) by chronic intermittent hypoxia (CIH), a defining characteristic of obstructive sleep apnea (OSA). This investigation seeks to elucidate the underlying mechanisms that contribute to the development of cardiovascular diseases in patients with OSA, with a particular focus on CIH-induced vascular aging. Methods The BioSpherix-OxyCycler system was used to establish models of CIH in both rats and human umbilical vein endothelial cells (HUVECs). To assess VECs senescence, various methods were employed including EdU incorporation assay, cell cycle analysis, senescence-associated β-galactosidase (SA-β-gal) staining, and senescence protein testing. Vascular aging was evaluated through measurements of carotid-femoral pulse wave velocity, intima-media thickness, and Ki67 immunohistochemical staining. In order to identify the molecular mechanisms associated with CIH-induced senescence in VECs, a bioinformatics study was conducted utilizing the Gene Expression Omnibus database. Results Under conditions of CIH, HUVECs exhibited inhibited proliferation, arrested cell cycle, increased activity of SA-β-gal, and elevated expression levels of p53 and p21 compared to HUVECs under normoxic conditions. Similarly, rats exposed to CIH displayed increased carotid-femoral pulse wave velocity, intima-media thickness, vascular permeability, and SA-β-gal activity in VECs, along with decreased expression of arterial Ki67. BTG3-associated protein (BANP) was found to be highly expressed in CIH-induced VECs. Furthermore, the overexpression of BANP resulted in the senescence of VECs, along with elevated levels of p53 phosphorylation and nuclear localization. Conclusions These findings demonstrate that CIH can induce VECs senescence and contribute to vascular aging. Additionally, BANP can induce VECs senescence by promoting p53 phosphorylation and nuclear retention. These discoveries offer novel insights into the increased cardiovascular risk associated with OSA, thereby presenting new possibilities for therapeutic intervention.

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“…The BANP gene was also mapped in CNVR 37. This gene encodes the BANP protein, which activates and regulates the transcription of genes involved in metabolism, DNA damage response, chromatin opening, and chromosomal segregation during mitosis [ 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of copy number variations in the genome of Dairy Gir cattle

Braga¹,

Chud

Watanabe³

et al. 2023

PLoS ONE

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Studying structural variants that can control complex traits is relevant for dairy cattle production, especially for animals that are tolerant to breeding conditions in the tropics, such as the Dairy Gir cattle. This study identified and characterized high confidence copy number variation regions (CNVR) in the Gir breed genome. A total of 38 animals were whole-genome sequenced, and 566 individuals were genotyped with a high-density SNP panel, among which 36 animals had both sequencing and SNP genotyping data available. Two sets of high confidence CNVR were established: one based on common CNV identified in the studied population (CNVR_POP), and another with CNV identified in sires with both sequence and SNP genotyping data available (CNVR_ANI). We found 10 CNVR_POP and 45 CNVR_ANI, which covered 1.05 Mb and 4.4 Mb of the bovine genome, respectively. Merging these CNV sets for functional analysis resulted in 48 unique high confidence CNVR. The overlapping genes were previously related to embryonic mortality, environmental adaptation, evolutionary process, immune response, longevity, mammary gland, resistance to gastrointestinal parasites, and stimuli recognition, among others. Our results contribute to a better understanding of the Gir breed genome. Moreover, the CNV identified in this study can potentially affect genes related to complex traits, such as production, health, and reproduction.

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Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina

Cited by 9 publications

References 98 publications

Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium

Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium

BANP Participates in the Chronic Intermittent Hypoxia-Induced Senescence of Vascular Endothelial Cells by Promoting P53 Phosphorylation and Nuclear Retention

Identification of copy number variations in the genome of Dairy Gir cattle

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