Barbituric Acid Sensor Based on Molecularly Imprinted Polymer‐ Modified Hanging Mercury Drop Electrode

Abstract

A barbituric acid sensor based on molecularly imprinted polymer-modified hanging mercury drop electrode is reported for sensitive and selective analysis in aqueous and blood plasma samples. It was developed by coating directly the barbituric acid-imprinted polymer, which is prepared from melamine and chloranil, onto the surface of a hanging mercury drop electrode under charge-transfer interaction at þ 0.3 V (vs. Ag/AgCl) in Model 303A electrode system connected with a polarographic analyzer/stripping voltammeter (PAR Model 264A). The binding event of barbituric acid was detected in the imprinted polymer layer through differential pulse, cathodic stripping voltammetric (DPCSV) signal at optimized operational conditions [accumulation potential þ 0.3 V (vs. Ag/AgCl), accumulation time 120 s, pH 7.0 (without supporting electrolyte), scan rate 10 mV s À1, pulse amplitude 25 mV]. The limit of detection for barbituric acid was found to be 0.54 ppm (RSD ¼ 3.7%, S/N ¼ 3). The detection was feasible without any problem of matrix effect or cross reactivity in the linearity range of concentration varying from 0.50 to 125.00 ppm of barbituric acid.

Analysis of Glucose by Potentiometry Using Electrode Carbon Paste/Molecularly Imprinted Polymer (Mip) With Metacrylic Acid as Monomer

“…10 The formed bond between monomers and glucose is hydrogen bonding which is easily broken so that glucose is easily extracted from the polymer chain. 11 The estimation of formed mold on the MIP can be seen in Figure - …”

Development of Uric Acid Sensor Based on Molecularly Imprinted Polymer-Modified Hanging Mercury Drop Electrode

“…The use of supporting electrolytes (KCl, NaNO 3 , borax, acetate, disodium hydrogen phosphate) was deliberately avoided to check anionic oxidation at positive potential. It is further known that borate and chlorate buffers, which are reportedly reactive particularly toward chloranil > C ¼ O of P(UA) polymer, cannot be used as a supporting electrolyte [18]. However, one may note that the physiological amount of such salts, if any, in the blood samples is insignificant to hamper our investigation.…”

“…The DPCSV resulted in superior peaks with baseline better than the linear sweep voltammetry. This entails an effective preconcentration step via facilitated multiple hydrogen-bonding in water than that in non-aqueous porogen (DMF) medium [18,20]. The filmencapsulated UA molecules are instantaneously oxidized at positive potential prior to DPCSV scan.…”

Detection of Oxidative Stress Biomarkers Using Novel Nanostructured Biosensors

“…The templated polymers show recognition properties resembling those found in biological receptors but they are more stable and considerably less expensive than biological systems (Malitesta et al, 2006). A range of molecularly-imprinted polymer-based sensors have been investigated using different transduction techniques, including: acoustic wave , Kugimiya et al, 1999, Liang et al, 2000, Matsuguchi et al, 2006, Percival et al, 2001, Yilmaz et al, 1999, potentiometry (Javanbakht et al, 2008), capacitance , conductometry (Kriz et al, 1996, Sergeyeva et al, 1999, voltammetry (Prasad et al, 2005), colorimetry (Stephenson et al, 2007), surface plasmon spectroscopy (Tokareva et al, www.intechopen.com 2006), and fluorescence (Chen et al, 2004, Jenkins et al, 2001, MorenoBondi et al, 2003 detection. Moreover, the molecularly-imprinted polymers can also be utilized for selective solid-phase separation techniques (Mahony et al, 2005, Masque et al, 2001, including electrophoresis and chromatography.…”