Barcode tagging of human oocytes and embryos to prevent mix-ups in assisted reproduction technologies

Novo, Sergi; Nogués, Carme; Penon, Oriol; Barrios, Leonardo; Santaló, Josep; Gómez-Martínez, Rodrigo; Estéve, J.; Errachid, Abdelhamid; Plaza, J.A.; Pérez‐García, Lluïsa; Ibáñez, Elena

doi:10.1093/humrep/det409

Cited by 20 publications

(25 citation statements)

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“…Therefore, a careful approach is required, because no studies with sufficiently long durations have been performed to ensure that there are no effects of electromagnetic waves [15][16][17][18][19]. Recent studies have investigated direct insertions into the zona pellucida and perivitelline space in each embryo using a barcode made of polysilicon with the goal of preventing mix-ups [20][21][22][23][24]. It has been reported that polysilicon barcode tagging can be used to identify individual mouse embryos.…”

Section: Discussionmentioning

confidence: 99%

Development of a security system for assisted reproductive technology (ART)

Hur

Ryu

Park

et al. 2014

J Assist Reprod Genet

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Purpose In the field of assisted reproductive technology (ART), medical accidents can result in serious legal and social consequences. This study was conducted to develop a security system (called IVF-guardian; IG) that could prevent mismatching or mix-ups in ART. Materials and methods A software program was developed in collaboration with outside computer programmers. A quick response (QR) code was used to identify the patients, gametes and embryos in a format that was printed on a label. There was a possibility that embryo development could be affected by volatile organic components (VOC) in the printing material and adhesive material in the label paper. Further, LED light was used as the light source to recognize the QR code. Using mouse embryos, the effects of the label paper and LED light were examined. The stability of IG was assessed when applied in clinical practice after developing the system. A total of 104 cycles formed the study group, and 82 cycles (from patients who did not want to use IG because of safety concerns and lack of confidence in the security system) to which IG was not applied comprised the control group. Results Many of the label paper samples were toxic to mouse embryo development. We selected a particular label paper (P touch label) that did not affect mouse embryo development. The LED lights were non-toxic to the development of the mouse embryos under any experimental conditions. There were no differences in the clinical pregnancy rates between the IG-applied group and the control group (40/104=38.5 % and 30/82=36.6 %, respectively). Conclusions The application of IG in clinical practice did not affect human embryo development or clinical outcomes. The use of IG reduces the misspelling of patient names. Using IG, there was a disadvantage in that each treatment step became more complicated, but the medical staff improved and became sufficiently confident in ART to offset this disadvantage. Patients who received treatment using the IG system also went through a somewhat tedious process, but there were no complaints. These patients gained further confidence in the practitioners over the course of treatment.

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Section: Discussionmentioning

confidence: 99%

Development of a security system for assisted reproductive technology (ART)

Hur

Ryu

Park

et al. 2014

J Assist Reprod Genet

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“…Another future perspective are silicone barcodes attached to the zona pellucida (Novo et al, 2013(Novo et al, , 2014, or similar embryo-based traceability innovations that, if demonstrated to be harmless, could also suit PGD/PGS cycles. Such technologies can increase safety beyond the level already guaranteed, from a patient-based perspective, by the EWS.…”

Section: Article In Pressmentioning

confidence: 99%

Failure mode and effects analysis of witnessing protocols for ensuring traceability during PGD/PGS cycles

Cimadomo¹,

Ubaldi

Capalbo

et al. 2016

Reproductive BioMedicine Online

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Preimplantation genetic diagnosis and aneuploidy testing (PGD/PGS) use is constantly growing in IVF, and embryo/biopsy traceability during the additional laboratory procedures needed is pivotal. An electronic witnessing system (EWS), which showed a significant value in decreasing mismatch occurrence and increasing detection possibilities during standard care IVF, still does not guarantee the same level of efficiency during PGD/PGS cycles. Specifically, EWS cannot follow single embryos throughout the procedure. This is however critical when an unambiguous diagnosis corresponds to each embryo. Failure Mode and Effects Analysis (FMEA) is a proactive method generally adopted to define tools ensuring safety along a procedure. Due to the implementation of a large quantitative PCR (qPCR)-based blastocyst stage PGD/PGS programme in our centre, and to evaluate the potential procedural risks, a FMEA was performed in September 2014. Forty-four failure modes were identified, among which six were given a moderate risk priority number (>15) (RPN; product of estimated occurrence, severity and detection). Specific corrective measures were then introduced and implemented, and a second evaluation performed six months later. The meticulous and careful application of such measures allowed the risks to be decreased along the whole protocol, by reducing their estimated occurrence and/or increasing detection possibilities.

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“…20 Thus, in this case we wanted to examine if the silicon nanowires integrated into the microbarcodes follow the same tendency. 20 Thus, in this case we wanted to examine if the silicon nanowires integrated into the microbarcodes follow the same tendency.…”

Section: The Evaluation Of the Embryo In Vitro Developmentmentioning

confidence: 99%

“…Embryos tagged with a single barcode were inspected every 24 h to assess the number of embryos that retained a barcode (retention rate). [16][17][18][19][20] For instance, just by adding 3 barcodes per embryo we can increase the retention rate up to 80% (in the case of the pushpin method). After the initial stage, the retention rate decreased following embryo development (Fig.…”

Section: The Barcode Retention On the Zpmentioning

confidence: 99%

“…6,7 Therefore, single embryo labelling and tracking, is a must in such applications. 18 We also tested this method with bovine 19 and human embryos, 20 where the studied embryos developed until the blastocyst stage at normal rates and high retention and identification rates were observed. 1,[8][9][10][11][12] However, many of them show complex fabrication technologies, can be harmful to living cells, or are designed to discriminate subpopulations of cells mixed in the same culture but not for individual cell labelling.…”

Section: Introductionmentioning

confidence: 99%

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Silicon-nanowire based attachment of silicon chips for mouse embryo labelling

et al. 2015

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The adhesion of small silicon chips to cells has many potential applications as direct interconnection of the cells to the external world can be accomplished. Hence, although some typical applications of silicon nanowires integrated into microsystems are focused on achieving a cell-on-a-chip strategy, we are interested in obtaining chip-on-a-cell systems. This paper reports the design, technological development and characterization of polysilicon barcodes featuring silicon nanowires as nanoscale attachment to identify and track living mouse embryos during their in vitro development. The chips are attached to the outer surface of the Zona Pellucida, the cover that surrounds oocytes and embryos, to avoid the direct contact between the chip and the embryo cell membrane. Two attachment methodologies, rolling and pushpin, which allow two entirely different levels of applied forces to attach the chips to living embryos, are evaluated. The former consists of rolling the mouse embryos over one barcode with the silicon nanowires facing upwards, while in the latter, the barcode is pushed against the embryo with a micropipette. The effect on in vitro embryo development and the retention rate related to the calculated applied forces are stated. Field emission scanning electron microscopy inspection, which allowed high-resolution imaging, also confirms the physical attachment of the nanowires with some of them piercing or wrapped by the Zona Pellucida and revealed extraordinary bent silicon nanowires.

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Barcode tagging of human oocytes and embryos to prevent mix-ups in assisted reproduction technologies

Abstract: This study was supported by the Sociedad Española de Fertilidad, the Spanish Ministry of Education and Science (TEC2011-29140-C03) and the Generalitat de Catalunya (2009SGR-00282 and 2009SGR-00158). The authors do not have any competing interests.

Cited by 20 publications

References 20 publications

Development of a security system for assisted reproductive technology (ART)

Development of a security system for assisted reproductive technology (ART)

Failure mode and effects analysis of witnessing protocols for ensuring traceability during PGD/PGS cycles

Silicon-nanowire based attachment of silicon chips for mouse embryo labelling

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