Barcoding for diatoms in the Yangtze River from the morphological observation and 18S rDNA polymorphic analysis

Li, Zheng; Liu, Xiaorong; Yu, Yizhen; Huang, Huijie; Li, Xingling; Ji, Qiang; Li, Kai; Yu, Youjia; Ding, Li; Mao, Zhengsheng; Pu, Yan; Chen, Peng

doi:10.1016/j.forsciint.2019.01.028

Cited by 13 publications

(3 citation statements)

References 7 publications

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“…DNA barcoding is a classification method that uses specific genetic makers to identify particular species and is becoming a new method for diatom species identification. A study by Li et al [21] first established a barcode for diatom morphology and diatom DNA sequences in the Yangtze River in Nanjing, China. The abundance and distribution frequency of each diatom species vary considerably among different water areas, which can support the determination of drowning sites.…”

Section: Diatom Species Classificationmentioning

confidence: 99%

Research advances in forensic diatom testing

Zhou

Cao

Huang

et al. 2020

Forensic Sciences Research

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In forensic practice, it is difficult to determine whether a dead body in the water resulted from drowning or from disposal after death. Diatom testing is currently an important supporting technique for the determination of death by drowning and of drowning sites, even though it is a time-consuming and laborious task. This article reviews the development of diatom testing over the decades and discusses a new method for the potential application of deep learning in diatom testing.

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Section: Diatom Species Classificationmentioning

confidence: 99%

Research advances in forensic diatom testing

Zhou

Cao

Huang

et al. 2020

Forensic Sciences Research

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“…Identifying diatoms using morphological methods beyond the genus level is difficult and requires expert knowledge. During the last years, a new concept of DNA barcode is becoming a promising approach to identify the tax of diatoms (Li et al, 2019). In this study, the authors performed the diatomic morphological analysis of diatoms to the level of genus using an optical and electron microscope.…”

Section: Discussionmentioning

confidence: 99%

Current state and prospects of forensic-medical diagnosis of drowning: A review of the literature

Voloshynovych

Kozan

Voloshynovych

et al. 2022

Problems of Forensic Sciences

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The article describes the current possibilities of forensic medicine in the diagnosis of death by drowning, as well as reflects the different views of the authors and the evolution of approaches to this issue. Despite the constant interest of scientists in the topic of drowning and extensive research on this issue, there are still many white spots. Researchers are trying to identify more specific changes characteristic of drowning and possible methods to detect them. The main evidence based method of diagnosing drowning at present is the detection of diatomic plankton in the tissues of isolated kidneys by light optical microscopy. However, microscopic examination and identification of diatomic plankton require a lot of time and accurate taxonomic examination. Also, a disadvantage of this method is that the methods of processing the material sent for research, as a result of the use of concentrated acids for the destruction of organs, greatly complicates and sometimes eliminates the possibility of detection of diatomic plankton. In some cases, the presence of diatoms during drowning is so small that it is impossible to detect them by microscopy. In such cases, the presence of phytoplankton DNA detected by PCR in tissues during drowning is almost the only method for diagnosing drowning. Nowadays there has been a lack of research in the field of forensic medicine related to the use of evidence-based medicine, especially in the field of drowning.

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“…More and more researches have concentrated on using these technologies to identify drowning. Previous studies had focused on the detection of the chlorophyllrelated genes of Euglena gracilis and Skeletonema costatum [13], 16S rRNA gene of Cyanobacterium [14], 18S rRNA, ITS, COI, rbcl, and universal plastid amplicon (UPA) genes of diatoms [15][16][17][18], as well as other specific genes of bacteria species [11,19,20]. However, in practical cases, the water collected from the discovery sites varied extremely in the types and quantities of planktons, especially for those cases of drowning in a bath or swimming pool with low diatom density.…”

Section: Introductionmentioning

confidence: 99%

Development and application of a multiplex PCR system for drowning diagnosis

Cheng

Xu²,

et al. 2021

Electrophoresis

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In recent years, the DNA detection of drowning‐related diatoms, cyanobacteria, and aeromonas has gradually attracted interest from forensic scientists. In this study, we described the validation and application of a novel multiplex PCR system. This system integrated 12 fluorescently labelled primers designed to amplify specific genes of diatoms, cyanobacteria, and aeromonas. The specificity studies demonstrated that this multiplex PCR system could detect nine species of diatom, seven species of cyanobacteria, and five species of aeromonas, all of which were drowning‐related and widely distributed in various water circumstance of southern China. The sensitivity studies indicated that the limit concentration of template DNA was 0.0125 ng. Besides, this multiplex PCR system had good performance in sizing precision and stability, but it is not suitable for degraded DNA samples. The application into forensic casework showed that all the tissue samples from ten nondrowning cases showed negative results, and the positive rates of lung, liver, kidney, and water samples from 30 drowning bodies were 100, 86.7, 90, and 100%, respectively. Combined with results of diatom tests of MD‐VF‐Auto SEM method, this multiplex PCR system could help rule out nondrowning bodies and provide extra evidences to support drowning diagnosis, especially for those cases with few diatoms observed. It is expected that this multiplex PCR system has great potential for forensic drowning diagnosis.

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Barcoding for diatoms in the Yangtze River from the morphological observation and 18S rDNA polymorphic analysis

Cited by 13 publications

References 7 publications

Research advances in forensic diatom testing

Research advances in forensic diatom testing

Current state and prospects of forensic-medical diagnosis of drowning: A review of the literature

Development and application of a multiplex PCR system for drowning diagnosis

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