Barcoding of ToLCV Resistant Tomato Germplasm in Bangladesh

Jannat, Mahbubah; Eva, Umma Homaira; Sarker, RH; Islam, Maksuda

doi:10.3329/ptcb.v30i1.47796

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…1). Additionally, previous research (Jannat et al 2020) reported favorable winter season production for Local Khustia 1, RHS1, and RHS2. These findings indicate variations in fruit yield among different tomato varieties under varying environmental conditions.…”

Section: Resultsmentioning

confidence: 72%

Genetic variability analysis of tomato varieties using rapd markers under heat stress

Anwar,

Roy,

Islam

2024

Bangladesh J. Bot.

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Heat stress threatens agricultural and food security by reducing yield and causing plant death. The use of intra-genetic diversity in heat stress responses is a potential avenue for harnessing tolerance to climate change circumstances. This study investigated the genetic diversity of twelve tomato genotypes in Bangladesh, focusing on their suitability for summer cultivation using seven Random Amplified Polymorphic DNA (RAPD) markers. The banding patterns obtained from the PCR results were used to identify genetic differences between individuals. The RAPD markers were found to show their effectiveness in discriminating the studied lines through the computation of average Polymorphism Information Content (PIC) values, particularly focusing on dominant markers with PIC values ranging from 0 to 0.5, emphasizing the informative nature of the employed primers. Cluster analysis grouped several genotypes with possible common ancestry. Overall, BARI Hybrid Tomato 4, BARI Hybrid Tomato 8, A12, D13, and RHS1 emerged as promising candidates for further genetic research and breeding programs, particularly in the context of heat-stress conditions during summer tomato cultivation in Bangladesh. Bangladesh J. Bot. 53(1): 91-100, 2024 (March)

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Section: Resultsmentioning

confidence: 72%

Genetic variability analysis of tomato varieties using rapd markers under heat stress

Anwar,

Roy,

Islam

2024

Bangladesh J. Bot.

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“…Dang et al (2021) also constructed a dendrogram using rbcL, matK and trnH-psbA and found two major clusters. Jannat et al (2020) used all the six Barcode primers and found similar type five main clades. Sharma et al (2012) worked with matK, rpoC1 and rbcL and constructed a bootstrap consensus phylogenetic tree based on the sequence obtained with the loci tested for.…”

Section: Resultsmentioning

confidence: 93%

Morphological and Genotypic Characterization Of Different Lotus (Nelumbo Nucifera Gaertn.) Samples Available in Bangladesh

Roy

Jannat

Tabassum

et al. 2022

Bangladesh J. Plant Taxon

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Asian lotus (Nelumbo nucifera Gaertn.), commonly known as sacred lotus is a basal eudicot. It has been grown and cultivated as food, medicine and for cultural, and religious activities. In the current study, samples were collected from six different locations to evaluate the variation among different lotus germplasm based on external morphological characteristics, as well as, to study the genetic variation and the molecular characterization. Analysis of variance showed a higher level of variations among the germplasm for all the morphological features. Based on the morphological features, a dendrogram was constructed to assess the linkage among the germplasm. The yellow lotus of Cumilla was considered superior among the germplasm studied. To assess the genetic diversity and the correct identification of lotus germplasm, molecular method “Barcoding” was performed. To achieve the goal, two plastidial regions: rpoB and rpoC1 were employed. The germplasm showing successful PCR were subjected to sequence analysis of their barcode genes. All the selected barcode genes showed successful identification of all the germplasm as N. nucifera in multilocus identification based on their sequences except for the germplasm of Rajshahi and also confirmed the yellowish lotus of Cumilla considered as a new cultivar N. nucifera ‘Gomoti’, newly found in Bangladesh. Genetic sequences obtained in the context of DNA barcoding had also been used to create a phylogenetic tree in which the germplasm were clustered into five main clades. The current study was successful in establishing an efficient protocol for the correct identification of lotus germplasm and was capable of establishing an elite gene source. Moreover, future studies are warranted to see the identifying capability and diverging power of the barcodes. Bangladesh J. Plant Taxon. 29(1): 85-95, 2022 (June)

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Barcoding of ToLCV Resistant Tomato Germplasm in Bangladesh

Cited by 2 publications

References 14 publications

Genetic variability analysis of tomato varieties using rapd markers under heat stress

Genetic variability analysis of tomato varieties using rapd markers under heat stress

Morphological and Genotypic Characterization Of Different Lotus (Nelumbo Nucifera Gaertn.) Samples Available in Bangladesh

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