Barcoding rotifer biodiversity in Mediterranean ponds using diapausing egg banks

Moreno, Emilio; Conde‐Porcuna, José M.; Gómez, África

doi:10.1002/ece3.2986

Cited by 18 publications

(11 citation statements)

References 109 publications

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“…Thus increasing the number of putative species in B. quadridentatus. Only five sequences from the Iberian Peninsula reported by Moreno et al (2017) fell within mitochondrial lineage G of this study (see Fig. S5).…”

Section: Discussionmentioning

confidence: 75%

Cryptic molecular diversity in the morphologically variable rotiferan Brachionus quadridentatus (Rotifera: Monogononta)

García-Morales

Domínguez‐Domínguez

2019

RBT

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Brachionus quadridentatus is a morphologically variable species distributed worldwide. Its taxonomy is confusing due the numerous infrasubspecific variants described in the taxon. Here we explore genetic diversity of certain B. quadridentatus populations, using sequences of COI mtDNA and 18S rDNA genes. With traditional morphology (taxonomic keys), the specimens identified fell into three recognized variants: B. quadridentatus quadridentatus, B. quadridentatus f. brevispinus and B. quadridentatus f. cluniorbicularis. The coalescent species delimitation analysis highly supports the presence of at least three putative species within B. quadridentatus complex in agreement with the phylogenetic result and GMYC analysis with the 18S gene. Nevertheless, the morphological and mitochondrial information show that the variation within each of the three putative species is much more extensive, finding seven monophyletic and highly divergent genetic groups (>10%) that have congruence with the morphotypes found in this study and ABGD analysis, which display clear morphological differences mainly in the diagnostic characters.

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Section: Discussionmentioning

confidence: 75%

Cryptic molecular diversity in the morphologically variable rotiferan Brachionus quadridentatus (Rotifera: Monogononta)

García-Morales

Domínguez‐Domínguez

2019

RBT

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“…Such high genetic distances within the same morphospecies is known in rotifers for cases of cryptic or pseudocryptic species (e.g. Mills et al, 2017;Moreno et al, 2017). Indeed, the use of a statistical approach to identify taxonomic units from DNA sequence data, such as the ABGD method we applied, revealed that several cryptic taxa could be potentially present in our dataset.…”

Section: Abgdmentioning

confidence: 62%

We are ready for faunistic surveys of bdelloid rotifers through DNA barcoding: the example of Sphagnum bogs of the Swiss Jura Mountains

Fontaneto¹,

Eckert²,

Anicic³

et al. 2019

Limnetica

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We are ready for faunistic surveys of bdelloid rotifers through DNA barcoding: the example of Sphagnum bogs of the Swiss Jura MountainsThe identification of biological diversity through DNA barcoding and metabarcoding of the organisms living in the field has the potential to revolutionise the way biological surveys and monitoring are performed. Yet, we still do not know if the current representativeness of the reference database of DNA sequence data is sufficient to allow such approaches. Here, we show that, at least for bdelloid rotifers (Metazoa; Rotifera; Bdelloidea) in Europe, current knowledge is ripe to perform such surveys. We show the results of an exercise performed on bdelloid rotifers in Sphagnum bogs of the Swiss Jura Mountain. The results of DNA-based identifications were rather consistent with the morphology-based identifications, and the few cases of mismatch could be used as a cautionary tale to avoid potential misinterpretations of results. The mismatches were due to cases of the closest match not being genetically very close, and to the occurrence of cryptic species.

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“…They found that resting stages of several zooplankton species hatched from these cores, including P. similis which was one of most abundant zooplankters at certain times of year. Moreno et al reported that Proales sp. hatched from DEs collected from sediments of Tinaja Lake located in Ruidera Natural Park in Central Spain.…”

Section: Discussionmentioning

confidence: 99%

Using Proales similis (Rotifera) for toxicity assessment in marine waters

Snell

Johnston

Matthews

et al. 2019

Environmental Toxicology

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There is a need to develop more animal species for assessing toxicity in marine environments. Cyst‐based toxicity tests using invertebrates are especially fast, technically simple, cost‐effective, and sensitive to a variety of toxicants. Over the past 30 years, a variety of toxicity endpoints have been measured using the marine rotifer Brachionus plicatilis hatched from cysts, including mortality, reproduction, ingestion, swimming, enzyme activity, and gene expression. A consensus has developed that the most ecologically relevant toxicity measurements should be made using more than one species. Furthermore, it has been noted that the rotifer species toxicant sensitivity distribution is much broader than which endpoint is measured. This implies that toxicity should be measured with the simplest, fastest, least expensive test available on as many species as feasible. If a battery of test species is to be used to estimate toxicity, diapause egg‐based toxicity tests that do not require culturing of test animals will be key. In this paper, we describe how diapause eggs of a new marine rotifer, Proales similis, can be produced, stored and hatched under controlled conditions to produce animals for toxicity tests. Methods are described for quantifying the toxicity of copper, mercury and cadmium based on mortality, ingestion, reproduction, and diapause egg hatching endpoints. We found that reproduction and ingestion endpoints were generally more sensitive to the metals than mortality or diapause egg hatching. When the copper sensitivity of P. similis was compared to Brachionus manjavacas and B. plicatilis using an ingestion test, similar EC50s were observed. In contrast, the B. rotundiformis ingestion EC50 for copper was about 4× more sensitive. Although diapause egg hatching was not the most sensitive endpoint, it is the most ecologically relevant for assessing sediment toxicity. Our discovery of diapausing eggs in the P. similis life cycle has created a conundrum. We have not observed males or sex in P. similis populations, which is a direct contradiction to the orthodox view of the monogonont rotifer life cycle. Work is needed to clarify how diapause egg production is accomplished by P. similis and whether sexual reproduction is involved.

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Barcoding rotifer biodiversity in Mediterranean ponds using diapausing egg banks

Cited by 18 publications

References 109 publications

Cryptic molecular diversity in the morphologically variable rotiferan Brachionus quadridentatus (Rotifera: Monogononta)

Cryptic molecular diversity in the morphologically variable rotiferan Brachionus quadridentatus (Rotifera: Monogononta)

We are ready for faunistic surveys of bdelloid rotifers through DNA barcoding: the example of Sphagnum bogs of the Swiss Jura Mountains

Using Proales similis (Rotifera) for toxicity assessment in marine waters

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