Barley ADH-1 modulates susceptibility to Bgh and is involved in chitin-induced systemic resistance

Käsbauer, Christoph; Pathuri, Indira Priyadarshini; Hensel, Goetz; Kumlehn, Jochen; Hückelhoven, Ralph; Proels, Reinhard K.

doi:10.1016/j.plaphy.2017.12.029

Cited by 18 publications

(13 citation statements)

References 44 publications

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“…hordei induces early upregulation of alcohol dehydrogenase 1 (ADH-1) activity in leaf epidermal tissue. Chitin-treatment induced systemic downregulation of ADH-1 activity and resistance to PM, while overexpression of ADH-1 inhibited the chitin-induced resistance to PM (Käsbauer et al 2018). …”

Section: Fungal Potential Against Host Immune Systemmentioning

confidence: 99%

Chitin and chitin-related compounds in plant–fungal interactions

Pusztahelyi

2018

Mycology

153

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Chitin is the second abundant polysaccharide in the world after cellulose. It is a vital structural component of the fungal cell wall but not for plants. In plants, fungi are recognised through the perception of conserved microbe-associated molecular patterns (MAMPs) to induce MAMP-triggered immunity (MTI). Chitin polymers and their modified form, chitosan, induce host defence responses in both monocotyledons and dicotyledons. The plants’ response to chitin, chitosan, and derived oligosaccharides depends on the acetylation degree of these compounds which indicates possible biocontrol regulation of plant immune system. There has also been a considerable amount of recent research aimed at elucidating the roles of chitin hydrolases in fungi and plants as chitinase production in plants is not considered solely as an antifungal resistance mechanism. We discuss the importance of chitin forms and chitinases in the plant–fungal interactions and their role in persistent and possible biocontrol.Abbreviations ET, ethylene; GAP, GTPase-activating protein; GEF, GDP/GTP exchange factor; JA, jasmonic acid; LysM, lysin motif; MAMP, microbe-associated molecular pattern; MTI, MAMP-triggered immunity; NBS, nucleotide-binding site; NBS-LRR, nucleotide-binding site leucine-rich repeats; PM, powdery mildew; PR, pathogenesis-related; RBOH, respiratory burst oxidase homolog; RLK, receptor-like kinase; RLP, receptor-like protein; SA, salicylic acid; TF, transcription factor.

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Section: Fungal Potential Against Host Immune Systemmentioning

confidence: 99%

Chitin and chitin-related compounds in plant–fungal interactions

Pusztahelyi

2018

Mycology

153

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“…With the development of sequencing technology, the genomes of many species can be analyzed, which promotes the identification of the plant ADH gene family at the genome-wide level. ADH family genes from tomato ( Moummou et al, 2012 ), rice ( Kitaoka et al, 2016 ), barley ( Kasbauer et al, 2018 ), Cucumis melon L. ( Jin et al, 2016 ) and Pyrus bretschneideri ( Qin et al, 2017 ) have been detected. Considering the disclosure of the wheat genome, it is possible to systematically identify the TaADH family members.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of alcohol dehydrogenase (ADH) gene family under waterlogging stress in wheat (Triticum aestivum)

Shen

Yuan

et al. 2021

PeerJ

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Background Alcohol dehydrogenase (ADH) plays an important role in plant survival under anaerobic conditions. Although some research about ADH in many plants have been carried out, the bioinformatics analysis of the ADH gene family from Triticum aestivum and their response to abiotic stress is unclear. Methods A total of 22 ADH genes were identified from the wheat genome, and these genes could be divided into two subfamilies (subfamily I and subfamily II). All TaADH genes belonged to the Medium-chain ADH subfamily. Sequence alignment analysis showed that all TaADH proteins contained a conservative GroES-like domain and Zinc-binding domain. A total of 64 duplicated gene pairs were found, and the Ka/Ks value of these gene pairs was less than 1, which indicated that these genes were relatively conservative and did not change greatly in the process of duplication. Results The organizational analysis showed that nine TaADH genes were highly expressed in all organs, and the rest of TaADH genes had tissue specificity. Cis-acting element analysis showed that almost all of the TaADH genes contained an anaerobic response element. The expression levels of ADH gene in waterlogging tolerant and waterlogging sensitive wheat seeds were analyzed by quantitative real-time PCR (qRT-PCR). This showed that some key ADH genes were significantly responsive to waterlogging stress at the seed germination stage, and the response of waterlogging tolerant and waterlogging sensitive wheat seeds to waterlogging stress was regulated by different ADH genes. The results may be helpful to further study the function of TaADH genes and to determine the candidate gene for wheat stress resistance breeding.

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“…Rice RAC1 functions in chitin-triggered immunity and is activated via the chitin-signalling receptor kinase CERK1 and RAC-GEF1, a member of a plant-specific RHO-GEF family [29]. Chitin is a potent elicitor of early defense reactions in barley and can induce systemic resistance to Bgh infection [10, 30]. However, it is unclear to what extent chitin elicitation contributes to basal resistance of barley in the authentic interaction with Bgh .…”

Section: Discussionmentioning

confidence: 99%

Barley ROP-INTERACTIVE PARTNER-a organizes into RAC1- and MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING PROTEIN 1-dependent membrane domains

Hoefle

McCollum

Hückelhoven

2019

Preprint

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8Small ROP (also called RAC) GTPases are key factors in polar cell development and in 9 interaction with the environment. ROP-Interactive Partner (RIP) proteins are predicted scaffold 10 or ROP-effector proteins, which function downstream of activated GTP-loaded ROP proteins 11 in establishing membrane heterogeneity and cellular organization. Grass ROP proteins 12 function in cell polarity, resistance and susceptibility to fungal pathogens but grass RIP proteins 13 are little understood. 14 We found that the barley (Hordeum vulgare L.) RIPa protein can interact with barley ROPs in 15 yeast. Fluorescent-tagged RIPa, when co-expressed with the constitutively activated ROP 16 protein CA RAC1, accumulates at the cell periphery or plasma membrane. Additionally, RIPa, 17 locates into membrane domains, which are laterally restricted by microtubules, when co-18 expressed with RAC1 and MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING 19 PROTEIN 1. Both structural integrity of MICROTUBULE-ASSOCIATED ROP-GTPASE 20 ACTIVATING PROTEIN 1 and microtubule stability are key to maintenance of RIPa-labeled 21 membrane domains. In this context, RIPa also accumulates at the interface of barley and 22 invading hyphae of the powdery mildew fungus Blumeria graminis f.sp. hordei. 23 Data suggest that barley RIPa interacts with barley ROPs and specifies RAC1 activity-24 associated membrane domains with potential signaling capacity. Lateral diffusion of this RAC1 25 signaling capacity is restricted the resulting membrane heterogeneity requires intact 26 microtubules and MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING PROTEIN 1. 27 Focal accumulation of RIPa at sites of fungal attack may indicate locally restricted ROP activity 28 at sites of fungal invasion. 29 30 Keywords: Arabidopsis thaliana, Hordeum vulgare, interactor of constitutive active ROPs, 31 membrane asymmetry, microtubule, RAC GTPase, ROP GTPase, susceptibility, resistance 32 In plants, ROP (RHO of plants) small GTPases are the only members of the RHO protein 35 family, which consists of several subfamilies (RHO, RAC, CDC42, Rnd und RhoBTB) in 36 mammals [1, 2]. ROPs organize a bunch of cellular processes as signaling GTPase. Among 37 the most prominent ROP-regulated events are the subcellular organization of the cytoskeleton 38 and vesicular traffic [3]. ROP-regulated cellular organization is crucial for normal plant 39 development e.g. in polar cell growth or asymmetric cell division but also in interaction with the 40 environment e.g. in regulation of stomata aperture or in interaction with pathogens. ROP 41 activity is tightly regulated via proteins that facilitate hydrolysis and exchange of ROP-bound 42 nucleotides. ROP-GDP is the signaling-inactive form of ROP and can be further controlled by 43 ROP-GDIs (ROP-guanine nucleotide dissociation inhibitors) that bind to ROP-GDP. ROP-44GDIs support cytosolic localization of ROPs most likely by direct binding of isoprenyl-residues 45 at the C-terminus of type I ROPs, which carry a CAAX-box prenylation motif. ROP-GDP further 46 can in...

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Barley ADH-1 modulates susceptibility to Bgh and is involved in chitin-induced systemic resistance

Cited by 18 publications

References 44 publications

Chitin and chitin-related compounds in plant–fungal interactions

Chitin and chitin-related compounds in plant–fungal interactions

Genome-wide identification of alcohol dehydrogenase (ADH) gene family under waterlogging stress in wheat (Triticum aestivum)

Barley ROP-INTERACTIVE PARTNER-a organizes into RAC1- and MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING PROTEIN 1-dependent membrane domains

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